Immunofluorescence analysis was carried out based on previously described [33 (link)]. A total of 1× 104 cells were plated on coverslips in 48-well plates and infected with shNC or shSPAG5. The cells were then incubated at 37°C for 24 hours and collected, and fixed with 4% paraformaldehyde for 30 min. Then, the cells were incubated with anti-E-cadherin antibody (cat no. ab11512, Abcam) at 4°C overnight. For Snail staining, the cells were penetrated with Trion-X 100 (Sigma-Aldrich, USA) and then incubated with anti-Snail antibody (cat no. ab167609). After that, the cells were probed with DAPI solution for 5 min. The cells were photographed using an immunofluorescence microscope (Olympus, USA).
Anti snail antibody
Manufactured by Merck Group
Sourced in United States
The Anti-Snail antibody is a laboratory reagent used in research applications. It is a specific antibody that binds to the Snail protein, which is a transcription factor involved in the epithelial-mesenchymal transition process. The antibody can be used in various techniques, such as Western blotting, immunohistochemistry, and immunofluorescence, to detect and analyze the expression of the Snail protein in biological samples.
Automatically generated - may contain errors
2 protocols using anti snail antibody
1
E-cadherin and Snail Immunofluorescence
2
Immunoblotting and Immunofluorescence Antibody Protocols
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