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2 protocols using lithium phenyl 2 4 6 trimethylbenzoylphosphinate lap 95

1

Tartrazine Pigment Cytotoxicity Evaluation

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Tartrazine pigment was purchased from GreenTech (Daejeon, Gyeonggi, South Korea). A live/dead cell viability kit was purchased from Invitrogen (Carlsbad, CA, USA). Dulbecco's modified Eagle's medium (DMEM), penicillin streptomycin (p/s), fetal bovine serum (FBS), l‐glutamine, and phosphate‐buffered saline (PBS, pH 7.4) were purchased from WelGene (Daegu, Gyeongbuk, South Korea). Beef (tenderloin) was purchased from Local market (Emart, Gwamgjin‐Gu, Seoul). Gelatin (Type A, 300 bloom from porcine skin), methacrylic anhydride (MA), lithium phenyl‐2,4,6‐trimethylbenzoylphosphinate (LAP, ≥ 95%), doxycycline, insulin, oleic acid, Oil Red O staining solution, bovine serum albumin, and Triton X‐100 were purchased from Sigma‐Aldrich (St. Louis, MO, USA). All other chemical agents used in this study were of analytical grade.
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2

Fabrication of Biomimetic Cell Scaffolds

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Sodium alginate was purchased from Junsei Chemical Co. (Tokyo, Japan). A live/dead cell viability kit was purchased from Invitrogen (Carlsbad, CA, USA). Tartrazine was purchased from GreenTech (Daejeon, South Korea). Horse serum and fungizone were purchased from Gibco (Grand Island, NY, USA). Dulbecco’s Modified Eagle’s medium (DMEM), penicillin-streptomycin (P/S), fetal bovine serum (FBS), L-glutamine, and phosphate-buffered saline (PBS; pH 7.4) were purchased from WelGene (Daegu, Gyeongbuk, South Korea). Zein powder, calcium chloride dihydrate, AESO, PEGDA (molecular weight: 575 g/mol), lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, ≥95%), collagenase I, entactin-collagen-laminin (ECL), insulin, linoleic acid, Nile red, bovine serum albumin, and Triton X-100 were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals used in this study were of analytical grade.
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