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Hyclone fetal bovine serum

Manufactured by GE Healthcare
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HyClone fetal bovine serum is a high-quality, sterile-filtered serum derived from the blood of fetal bovine calves. It is commonly used as a supplement in cell culture media to support the growth and proliferation of various cell lines.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (14 mentions) Glutamine, by Thermo Fisher Scientific (6 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (5 mentions) Gentamicin, by Thermo Fisher Scientific (5 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) Mda mb 231, by American Type Culture Collection (3 mentions) Mda mb 468, by American Type Culture Collection (3 mentions) Glutamax, by Thermo Fisher Scientific (3 mentions) E myco plus kit, by iNtRON Biotechnology (3 mentions) Rpmi media, by Thermo Fisher Scientific (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Bt474, by American Type Culture Collection (2 mentions) Mcf 7, by American Type Culture Collection (2 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions) M csf, by Thermo Fisher Scientific (2 mentions) Horse serum, by Thermo Fisher Scientific (2 mentions) Ham s f 10 nutrient mix, by Thermo Fisher Scientific (2 mentions) Crl 4001, by American Type Culture Collection (2 mentions) Mycoscope kit, by Genlantis (2 mentions) Fetal bovine serum (fbs), by Welgene (1 mentions)

Close spelling variants of this product

HyClone (1208 citations) HyClone fetal bovine serum (FBS, (28 citations) Bovine serum (4 citations) HyClone defined fetal bovine serum (4 citations) HyClone characterized fetal bovine serum (FBS) (4 citations) HyClone 5% fetal bovine serum (3 citations) HyClone 10% fetal bovine serum (3 citations) HyClone fetal bovine serum (HI-FBS; (2 citations) HyClone Research Grade Fetal Bovine Serum (2 citations) Hyclone™ 10% fetal bovine serum (FBS; (2 citations)

53 protocols using hyclone fetal bovine serum

1

Cell Culture Protocols for HEK293T and SUPT1 Cells

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HEK293T cells (ATCC, CRL-3216) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco; catalog no. 11965092) with 10% HyClone fetal bovine serum (GE Healthcare; catalog no. SH30910.03) and 1% penicillin-streptomycin (Gibco; catalog no. 15140122) at 37°C in a humidified CO2 incubator. SUPT1 cells, acquired from ATCC (CRL-1942), were maintained similarly but in RPMI medium (Gibco; catalog no. 11875093), 10% HyClone fetal bovine serum (GE Healthcare; catalog no. SH30910.03), 1% penicillin-streptomycin (Gibco; catalog no. 15140122), and 10 mM HEPES. HEK293T cells were plated in 6-well dishes for transfections at a density of 1.5 × 105 cells/ml.
McDonnell M.M., Crawford K.H., Dingens A.S., Bloom J.D, & Emerman M. (2020). APOBEC3C Tandem Domain Proteins Create Super Restriction Factors against HIV-1. mBio, 11(2), e00737-20.
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2

Vascular Calcification Protocol in RVSMCs

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Primary RVSMCs were extracted from 6-week-old Sprague-Dawley rats and cultured in DMEM (high glucose; WELGENE) with 10% HyClone fetal bovine serum (GE Healthcare Life Sciences). Cells were cultured from the third to the seventh passage and divided into four 100-mm dishes (Eppendorf). To induce VC, 2 mM Pi (pH 7.4) (Sigma) was used to treat RVSMCs for 6 h, 3 days, or 6 days in four different dishes, as published previously.36 (link) The RNAs of all samples were isolated simultaneously. A cell line of RVSMCs, A10 cells (CRL-1476, ATCC), were cultured using DMEM, high glucose, with 10% fetal bovine serum (WELGENE) for transfection and calcium assay. The HCT-116 cells were cultured using McCoy’s 5A media (WELGENE) with 10% HyClone fetal bovine serum (GE Healthcare Life Sciences) to perform luciferase reporter assay. Human coronary artery smooth muscle cells (Thermo Fisher Scientific) were cultured up to 4–8 passages using Medium 231 supplemented with Smooth Muscle Growth Supplement or Smooth Muscle Differentiation Supplement.
Ryu J., Kwon D.H., Choe N., Shin S., Jeong G., Lim Y.H., Kim J., Park W.J., Kook H, & Kim Y.K. (2019). Characterization of Circular RNAs in Vascular Smooth Muscle Cells with Vascular Calcification. Molecular Therapy. Nucleic Acids, 19, 31-41.
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3

Maintenance of HEK293T and THP-1 cells

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HEK293T cells were maintained in DMEM media (Life Technologies) with 1% penicillin-streptomycin (Gibco) and 10% heat-inactivated Hyclone fetal bovine serum (FBS, GE Healthcare). THP-1 human monocytes were maintained in RPMI media (Life Technologies) with 1% penicillin-streptomycin (Gibco) and 10% heat-inactivated Hyclone fetal bovine serum (FBS, GE Healthcare).
Amatullah H., Fraschilla I., Digumarthi S., Huang J., Adiliaghdam F., Bonilla G., Wong L.P., Rivard M.E., Beauchamp C., Mercier V., Goyette P., Sadreyev R.I., Anthony R.M., Rioux J, & Jeffrey K.L. (2022). Epigenetic reader SP140 loss of function drives Crohn’s disease due to uncontrolled macrophage topoisomerases. Cell, 185(17), 3232-3247.e18.
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4

Cell Culture and Transfection Protocol

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HEK293T cells (ATCC, CRL-3216) were cultured in DMEM (Gibco, #11965092) with 10% HyClone Fetal Bovine Serum (GE Healthcare, #SH30910.03) and 1% penicillin-streptomycin (Gibco; #15140122) at 37°C in a humidified CO2 incubator. SUPT1 cells, acquired from ATCC (CRL-1942), were maintained similarly but in RPMI medium (Gibco, #11875093), 10% HyClone Fetal Bovine Serum (GE Healthcare, #SH30910.03), 1% penicillin-streptomycin (Gibco, #15140122), and 10mM HEPES. HEK293T cells were plated in 6-well dishes for transfections at a density of 1.5x10 5 cells per 1mL. Transfections were performed with the TransIT-LT1 transfection reagent (Mirus, #MIR2304) at a reagent:plasmid DNA ratio of 3:1.
McDonnell M.M., Crawford K.H., Dingens A.S., Bloom J.D., & Emerman M. (2020). APOBEC3C tandem domain proteins create super restriction factors against HIV-1.
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5

Isolation and Characterization of Minipig PDLSCs

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The minipig PDLSCs were isolated and cultured under the same conditions employed in our previous study for human PDLSCs26 (link). Periodontal ligament tissue was scraped from the middle third of the root surface of the incisors in minipigs and was digested with collagenase type I (3 mg/mL; Worthington Biochemical, USA) and dispase II (neutral protease, 4 mg/mL; Roche Diagnostics, USA) for 2 h at 37 °C to obtain a single-cell suspension. After isolation, the cells were cultured in alpha minimum essential medium (Gibco, Thermo Fisher Scientific, Waltham, MA) with 20% fetal bovine serum (Hyclone; GE Healthcare Life Sciences, Logan, UT), 100 U/mL penicillin/streptomycin, 2 mM glutamine, 55 mM 2-ME (Gibco), and 0.1 mM L-ascorbic acid phosphate (Wako Chemicals, Richmond, VA) at 37 °C in 5% CO2. The 3rd passage of PDLSCs was used in the following experiments. The multipotency of the PDLSCs was confirmed by examining osteogenicity using Alizarin red S staining and adipogenicity using Oil red O staining (Fig. S1).
Zhang C., Yan B., Cui Z., Cui S., Zhang T., Wang X., Liu D., Yang R., Jiang N., Zhou Y, & Liu Y. (2017). Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells. Scientific Reports, 7, 10519.
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6

PANC-1 Cell Response to Grape Seed Procyanidins

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The human PC cell line PANC-1 was obtained from Procell (Wuhan, China) (http://www.procell.com.cn/) and cultured in monolayers in Dulbecco's modified Eagle's medium (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) in a humidified incubator at 37°C and 5% CO2. The GSP extract, obtained from JF-NATURAL (Tianjin, China; catalog no. J011003), contained monomeric (9.5%), dimeric (12.8%), trimeric (76.7%) and oligomeric (1%) procyanidins. The 100 µg GSPs were dissolved in 100 µl dimethylsulfoxide (DMSO; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) for 10 min at room temperature prior to addition to cell culture media. The maximum concentration of DMSO in the media did not exceed 0.1%. PANC-1 cells were treated with 20 µg/ml GSP for 3, 12 and 24 h at 37°C, and the treated cells were used to prepare for SS3, SS12 and S24 samples, respectively. Additionally, control cell samples were treated with DMSO for 3, 12 and 24 h at 37°C, and then SC3, SC12 and SC24 samples were prepared accordingly.
Wang W., Zhan L., Guo D., Xiang Y., Tian M., Zhang Y., Wu H., Wei Y., Ma G, & Han Z. (2019). Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression. Oncology Letters, 17(3), 2777-2787.
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7

Culturing Normal and DMD Myoblasts

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Cryopreserved normal human myoblasts were purchased from Lonza Inc (Mapleton, IL, USA), while DMD-affected myoblast were purchased from Axol Bioscience Ltd. (Little Chesterford, UK). Myoblasts (MB) were cultured in standard conditions in specific Skeletal Muscle Cell Growth Medium-2 (SkGM-2 bulletkit, Lonza Inc, Mapleton, IL, USA) supplemented with human Epidermal Growth Factor (hEGF), 0.5 ml; Fetuin, 5.0 ml; Bovine Serum Albumin (BSA), 5.0 ml; Dexamethasone, 0.5 ml; Insulin, 5.0 ml; Gentamicin/Amphotericin B (GA), 0.5 ml. Culture medium was changed twice a week and upon reaching 60–70% confluence, myoblasts were harvested and passaged using mechanical and enzymatic dissociation methods of 5 min incubation with 0.25% trypsin EDTA (Gibco-ThermoFischer, Waltham, MA USA). Enzymatic activity for cell detachment was stopped with culture media supplemented with 10% FBS (fetal bovine serum, Hyclone, GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Next, the cells were washed twice. Human MBs were harvested between passages 5–7, which is the optimal passage for ex vivo cell fusion procedure.
Siemionow M., Cwykiel J., Heydemann A., Garcia J., Marchese E., Siemionow K, & Szilagyi E. (2018). Dystrophin Expressing Chimeric (DEC) Human Cells Provide a Potential Therapy for Duchenne Muscular Dystrophy. Stem Cell Reviews, 14(3), 370-384.
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8

Culturing Vascular Tissue in Bioreactor

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Both ends of the artery were sutured onto glass arms of the bioreactor, then the arms with the arteries were mounted on an autoclaved bioreactor chamber with a circulating system. The bioreactor system was filled with endothelial cell-smooth muscle cell (EC-SMC) medium consisting of Vasculife and Dulbecco’s Modified Eagle Medium (Gibco) (1:1) supplemented with 2.5% Fetal Bovine Serum (HyClone, GE Healthcare Life Sciences), 1% Gibco Antibiotic-Antimycotic (Life Technologies), and dextran (30 g/L, Sigma). This pseudo-physiological medium had a viscosity similar to that of whole blood. The original pH value of growth medium is 7.8±0.1.
To collect local medium in the vessel for in-situ and real-time monitoring of local pH and ion concentrations, a 22 g × 15 cm-long needle (Cadence Science) with an injection site was inserted through the chamber cap and into the vascular lumen. Three air filters (PURADISC 25 AS Disposable Filter Device, GE Healthcare Life Sciences) were connected to the tubes of the chamber cup. The bioreactor system was moved from the biosafety cabinet to the incubator (37 °C, 5% CO2). Sixty percent of the medium was changed every other day. The entire culture time was 7 days.
Wang J., Kural M.H., Wu J., Leiby K.L., Mishra V., Lysyy T., Li G., Luo J., Greaney A., Tellides G., Qyang Y., Huang N, & Niklason L.E. (2021). An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies. Biomaterials, 275, 120911.
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9

Cryopreservation recovery medium protocol

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Universal cryopreservation recovery medium (81015) was purchased from In Vitro ADMET Laboratories, Inc. (Columbia, MD, USA). Fetal bovine serum Hyclone was purchased from GE Healthcare (Diegem, Belgium). William’s E medium (no phenol red, Gibco A12176) and insulin-transferrin-selenium (41400045) were provided by ThermoFisher Scientific (Merelbeke, Belgium). L-glutamine-penicillin-streptomycin solution (G1146), dexamethasone (D1756), glycochenodeoxycholic acid (G0759), deoxycholic acid (30970), chenodeoxycholic acid (C9377), glycocholic acid (G2878), glycodeoxycholic acid (361311), dimethyl sulfoxide (472301), paraquat (PQ, 36541), tartrazine (TAR, T0388), triclosan (TRI, 72779) were purchased from Sigma-Aldrich (Overijse, Belgium). Cyclosporine-A (CyA, 239835) was purchased from Calbiochem (Darmstadt, Germany).
Vilas-Boas V., Gijbels E., Leroy K., Pieters A., Baze A., Parmentier C, & Vinken M. (2021). Primary Human Hepatocyte Spheroids as Tools to Study the Hepatotoxic Potential of Non-Pharmaceutical Chemicals. International Journal of Molecular Sciences, 22(20), 11005.
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10

Culture and Maintenance of H9C2 Cardiac Myoblasts

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The H9C2 rat cardiac myoblast cell line was acquired from Cobioer Biosciences Co., Ltd. (Nanjing, China). Cells were maintained in complete high-glucose Dulbecco's Modified Eagle Medium (DMEM; Beijing Solarbio Science & Technology Co, Ltd., Beijing, China) supplemented with 10% fetal bovine serum (Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) and 1% penicillin-streptomycin (Beijing Leagene Biotechnology Co., Ltd., Beijing, China) in an incubator containing 95% humidified air and 5% CO2 at 37°C.
Shen P., Chen J, & Pan M. (2018). The protective effects of total paeony glycoside on ischemia/reperfusion injury in H9C2 cells via inhibition of the PI3K/Akt signaling pathway. Molecular Medicine Reports, 18(3), 3332-3340.
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