Solvent purity LC-MS grade as methanol (MeOH), acetonitrile (ACN), 2-propanol (IPA), acetone (ACE), dimethylsulfoxide (DMSO) and standards of mycotoxins T-2 toxin and HT-2 toxin were purchased from Sigma-Aldrich (Prague, Czech Republic). The stock solution standards of mycotoxins (100 µg/mL in acetonitrile) were prepared in 10% methanol/water (v/v) at a concentration of 1 µg/mL and stored at 4 °C in the refrigerator before use. Sodium chloride was purchased from Lachner (Neratovice, Czech Republic). Additives for LC-MS (purity LC-MS) as ammonium acetate, formic acid, acetic acid and citric acid (purity ≥ 99.5%) were purchased from Sigma-Aldrich (Prague, Czech Republic). Check-Sample-Survey, milk thistle powder obtained from Central Institute for Supervising and Testing in Agriculture (ÚKZÚZ Brno, Czech Republic). Ultrapure water was produced by Aqua Osmotic 06 (Tišnov, Czech Republic). Immunoaffinity columns R-Biopharm EASI-EXTRACT® T-2 & HT-2 were obtained from Jemo Trading (Bratislava, Slovak Republic).
Sodium chloride (nacl)
Manufactured by Lach-Ner
Sourced in Czechia
Sodium chloride is a chemical compound composed of sodium and chloride. It is a white, crystalline solid that is commonly known as table salt. Sodium chloride has a variety of laboratory applications, where it is used as a standard reference material, a buffer solution, and a source of sodium and chloride ions.
Automatically generated - may contain errors
Lab products found in correlation
Potassium chloride (kcl), by Lach-Ner (5 mentions)
Na2hpo4, by Merck Group (4 mentions)
Acetonitrile, by Merck Group (3 mentions)
Sodium hydroxide (naoh), by Lach-Ner (3 mentions)
Hydrochloric acid (hcl), by Lach-Ner (3 mentions)
K2s2o8, by Lach-Ner (3 mentions)
Methanol, by Merck Group (2 mentions)
Formic acid, by Merck Group (2 mentions)
Magnesium sulfate anhydrous, by Lach-Ner (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Methanol, by Lach-Ner (2 mentions)
Kh2po4, by Lach-Ner (2 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Merck Group (2 mentions)
Na2co3, by Merck Group (2 mentions)
2 2 azobis 2 methylpropionitrile, by Merck Group (2 mentions)
Milli q gradient a10 system, by Merck Group (2 mentions)
Ethyl acetate, by ITW Reagents (2 mentions)
Sodium dodecyl sulphate (sds), by Thermo Fisher Scientific (2 mentions)
Dinitrophenylhidrazine, by Thermo Fisher Scientific (2 mentions)
16 protocols using sodium chloride (nacl)
1
Quantitative Analysis of T-2 and HT-2 Toxins
2
Oxidative Stress Assay Chemicals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The chemicals used for the oxidative stress assays were thiobarbituric acid (Carlo Erba, Val de Reuil, France, CAS 504-17-6), sodium hydroxide (Lachner, Neratovice, Czech Republic, CAS 1310-73-2), (Ethylenedinitrilo)tetraacetic acid disodium salt (Lachner, Czech Republic, CAS 6381-92-6), sulfanilic acid (Acros Organics, Geel, Belgium, CAS 121-57-3), n-1-naphthyl ethylenediamine dihydrochloride (Fisher Chemicals, Loughborough, UK, CAS 1465-25-4), sodium chloride (Lachner, Czech Republic, CAS 7647-14-5), gelatine (Acros Organics, CAS 9000-70-8), nitrotetrazolium blue chloride (Acros Organics, CAS 298-83-9), horseradish peroxidase (Sigma Aldrich, St. Louis, MO, USA, CAS 9003-99-0), Tris(hydroxymethyl)aminomethane (Acros Organics, CAS 77-86-1), Potassium dihydrogen phosphate (Lachner, Czech Republic, CAS 7778-77-0), glucose (Lachner, Czech Republic, CAS 50-99-7), phenol red (Acros Organics, CAS 143-74-8), metaphosphoric acid (Acros Organics, CAS 37267-86-0), di-sodium hydrogen phosphate (Carlo Erba, France, CAS 7558-79-4), 5,5-dithio-bis-(2-nitrobenzoic acid) (Sigma Aldrich, USA, CAS 69-78-3), trisodium citrate dihydrate (Fisher Chemicals, UK, CAS 6132-04-3), Glutathione reduced (Acros Organics, CAS 70-18-8), and L-Epinephrine (Sigma Aldrich, USA, CAS 51-43-4).
3
Curcumin-loaded Polymeric Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Curcumin, polycaprolactone (average mol wt 45,000), poly(vinyl alcohol) (87–90% hydrolyzed, average mol wt 30,000–70,000), and fluorescein isothiocyanate (FITC) were purchased from Sigma-Aldrich (St Louis, MO, USA). Eudragit® FS 100 was a kind gift from Evonik Nutrition&Care GmbH (Darmstadt, Germany). Dichloromethane, dimethyl sulfoxide, and acetonitrile were purchased from Merck KGaA (Darmstadt, Germany). Ethyl acetate was purchased from Chimopar (Bucharest, Romania). Potassium dihydrogen phosphate and sodium hydroxide were purchased from Chemical Company (Iași, Romania). Sodium chloride was purchased from Lach-Ner (Brno, Czech Republic). All other chemicals were of analytical grade.
4
Quantification of Phenolic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ethyl gallate, 4-hydroxybenzaldehyde, vanillin, tyrosol, protocatechuic aldehyde, syringaldehyde, pyrogallol, 4-hydroxy-3-methoxycinnamaldehyde, 4-methylcatechol, tryptophol, ethyl 3,4-dihydroxycinnamate, 2,6-dimethoxyphenol, ethyl vanillate, homovanillyl alcohol, eugenol, 4-hydroxy-3-methoxyphenylacetone, salicylaldehyde, coniferyl alcohol, epicatechin, catechin, scopoletin, p-coumaric acid, caffeic acid, resveratrol, syringic acid, rutin, gallic acid, salicylic acid, protocatechuic acid, 2-methoxy-4-vinylphenol, 4-vinylphenol, 4-ethylguaiacol were purchased from Sigma-Aldrich (Steinheim, Germany). All chemicals were used in the highest purity available. Acetonitrile and methanol (gradient grade for liquid chromatography) were also purchased from Sigma-Aldrich. Deionized water was produced by Mili-Q® Reference System (Merck KGaA, Darmstadt, Germany). Sodium chloride was purchased by Lach-Ner (Neratovice, Czech Republic). Standard solution of n-alkanes C8-C33 was purchased by CPAchem (Stara Zagora, Bulgaria). Technical gases, i.e., helium 4.6, hydrogen, and nitrogen, were purchased from Linde Gas (Prague, Czech Republic).
5
Trace-level Quantification of Fluoroquinolones
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Citric acid monohydrate (≥ 99%), disodium hydrogen phosphate dodecahydrate (≥ 99%), EDTA (Ethylendiaminetetraacetic acid; p.a.), sodium chloride (p.a.), magnesium sulfate anhydrous (p.a.), Sodium sulfate anhydrous (p.a.) and hydrochloric acid (35%) were purchased from Lach:ner (Czech Republic). Magnesium nitrate hexahydrate (> 99%), ammonium (25%), methanol (LC–MS grade), acetonitrile (LC–MS grade), and water (LC–MS grade) were purchased from VWR (USA). Sodium hydroxide (> 98%) was purchased from Penta. Formic acid (LC–MS grade), enrofloxacin (> 99%), ciprofloxacin (> 98%), enrofloxacin-d5 (> 99%), and ciprofloxacin-d8 (> 99%) were purchased from Sigma Aldrich (USA). OASIS HLB (Hydrophylic-Lipophilic Balanced) cartridges (200 mg; 6 mL; particle diameter 25–35 μm) were purchased from WATERS (USA). Nitrogen (4.7) and argon (5.0) gas were purchased from Siad (Czech Republic). Nylon syringe filters (13 mm, 0.22 μm) were purchased from Chromservis (Czech Republic). Physico-chemical properties of FQs can be found in Table S1 .
6
Lipid Peroxidation Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chloroform, methanol, ammonium thiocyanate, iron(II) sulphate, cumene hydroperoxide, bovine haemoglobin, 2,6-di-tert-butyl-4-methylphenol (BHT), 1,1,3,3-tetraethoxypropane (TEP), phosphoric acid, plate count agar, peptone water (all from Sigma-Aldrich, Merck, St. Louis, MO, USA), sodium chloride, acetone (both from Lach-Ner, Neratovice, Czech Republic), hydrochloric acid, barium chloride dihydrate (both from Penta, Prague, Czech Republic), trichloracetic acid (TCA; Fisher Chemical, Leichestershire, UK), 2-thiobarbituric acid (TBA; Merck, Darmstadt, Germany) were used.
7
Pesticide Analysis in Citrus Fruits
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The pesticide standards were purchased from Restek (Bellefonte, Pennsylvania, USA) and Lab Instruments (Castellana Grotte, Italy). HPLC-MS-grade acetonitrile, methanol, formic acid, and acetic acid were obtained from Lachner (Neratovice, Czech Republic). Sodium chloride, magnesium sulfate anhydrous, disodium hydrogen citrate sesquihydrate (C6H8Na2O8), and trisodium citrate dihydrate (C6H5O7Na3 × 2H2O) were obtained from Lachner (Czech Republic). The graphitized carbon black (GCB), C18 sorbent, and primary and secondary amines (PSA) were supplied from Sigma-Aldrich (St Louis, Missouri, USA).
The selected citrus fruits were purchased from local markets in Belgrade, Serbia, in February 2022, and a total of 76 samples were analyzed (28 oranges, 26 lemons, 17 tangerines, and 5 grapefruits). Each fruit sample was analyzed as the whole fruit following European Union Guidelines Regulation, (EC) No. 396/2005 Annex [20 ]. During the survey, fruits were stored in fridges in labeled plastic bags at 4 °C until analysis.
The selected citrus fruits were purchased from local markets in Belgrade, Serbia, in February 2022, and a total of 76 samples were analyzed (28 oranges, 26 lemons, 17 tangerines, and 5 grapefruits). Each fruit sample was analyzed as the whole fruit following European Union Guidelines Regulation, (EC) No. 396/2005 Annex [20 ]. During the survey, fruits were stored in fridges in labeled plastic bags at 4 °C until analysis.
8
Bacterial Culture Media Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All compounds were commercial products: hydrochloric acid, sodium chloride, and sodium hydroxide (Lach-Ner, Neratovice, Czech Republic), sodium and potassium phosphates, (Penta, Praha, Czech Republic), tryptone (Oxoid, Basingstoke, UK), yeast extract, kanamycin, and tetramethoxysilane (TMOS) (Sigma-Aldrich, St. Louis, MO, USA).
Phosphate buffered saline (PBS) (pH 7.4) was prepared by dilution from a previously prepared concentrated solution (×10) of PBS that contained KH2PO4 (17 mmol/L), Na2HPO4 (52 mmol/L), and NaCl (1.5 mol/L). Luria–Bertani media (LB) contained tryptone (10 g/L), yeast extract (5 g/L), and NaCl (10 g/L), pH 7.2 [28 ]. The LB + kan50 medium was prepared by the addition of a stock kanamycin solution (10 g/L) to a final concentration of 50 mg/L after autoclaving. Minimal salts medium (MSM) contained MgSO4·7H2O (0.1 g/L), NH4NO3 (0.2 g/L), trace elements (0.1 mL), 0.5 M phosphate buffer, pH 7.2 (100 mL), and distilled water (900 mL) [27 ].
Phosphate buffered saline (PBS) (pH 7.4) was prepared by dilution from a previously prepared concentrated solution (×10) of PBS that contained KH2PO4 (17 mmol/L), Na2HPO4 (52 mmol/L), and NaCl (1.5 mol/L). Luria–Bertani media (LB) contained tryptone (10 g/L), yeast extract (5 g/L), and NaCl (10 g/L), pH 7.2 [28 ]. The LB + kan50 medium was prepared by the addition of a stock kanamycin solution (10 g/L) to a final concentration of 50 mg/L after autoclaving. Minimal salts medium (MSM) contained MgSO4·7H2O (0.1 g/L), NH4NO3 (0.2 g/L), trace elements (0.1 mL), 0.5 M phosphate buffer, pH 7.2 (100 mL), and distilled water (900 mL) [27 ].
9
Synthesis and Characterization of 5-FU Nanocarriers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chemicals
used for the synthesis of the different samples synthesized herein
are anhydrous AlCl3 (Sigma-Aldrich, 98%) and MgCl2 (Alfa Aesar, 99%) as metal precursors, NaOH (VWR Chemicals, 99.1%)
as precipitating agent, 5-FU (Sigma, >99%) as a model anticancer
drug,
phosphate buffer (Na2HPO4 Sigma-Aldrich >99%;
NaH2PO4 Honeywell >99%), distillated water
(Thermo
Scientific), EtOH (Honeywell, 99.8%), NaCl (Lachner, 99.97%), KCl
(Lachner, 100%), MgCl2·6H2O (Roth, 99%),
and CaCl2 (Lachner, 96.3%).
used for the synthesis of the different samples synthesized herein
are anhydrous AlCl3 (Sigma-Aldrich, 98%) and MgCl2 (Alfa Aesar, 99%) as metal precursors, NaOH (VWR Chemicals, 99.1%)
as precipitating agent, 5-FU (Sigma, >99%) as a model anticancer
drug,
phosphate buffer (Na2HPO4 Sigma-Aldrich >99%;
NaH2PO4 Honeywell >99%), distillated water
(Thermo
Scientific), EtOH (Honeywell, 99.8%), NaCl (Lachner, 99.97%), KCl
(Lachner, 100%), MgCl2·6H2O (Roth, 99%),
and CaCl2 (Lachner, 96.3%).
10
Antioxidant Capacity Evaluation of Hops
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, 97%) and n-alkane standard solution C7-C30 (1000 μg/mL each component in hexane) were purchased from Sigma-Aldrich Chemie (Taufkirchen, Germany); 2-(3-hydroxy-6-oxo-xanthen-9-yl)benzoic acid, fluorescein (FL) and 2,2′-azobis-2-methyl-propanimidamide dihydrochloride (AAPH) were from Fluka Analytical (Bornem, Belgium); NaCl, KCl, KH2PO4 and K2S2O8 were from Lach-Ner (Brno, Czech Republic); Na2HPO4 was from Merck KGaA (Darmstadt, Germany); carbon dioxide and nitrogen gases (99.9%) were from Gaschema (Jonava, Lithuania). International Calibration Extract 4 (ICE-4), containing α- and β-acids (10.98% of cohumulone; 31.60% of humulone+adhumulone; 13.02% colupulone; 13.52% lupulone+adlupulone), was obtained from Labor Veritas AG (Zürich, Switzerland). Divinylbenzene/Carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fibers and 20 mL SPME vials were purchased from Supelco (Bellefonte, PA, USA). All solvents were of analytical and HPLC-grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!