Blood samples were collected into EDTA and serum tubes and were centrifuged for 15 min at 1000g at 4°C and 21°C, respectively. Aliquots were immediately frozen in liquid nitrogen and subsequently stored at −80°C until analysis. Plasma glucose, FFA, TAG and total cholesterol were analyzed with standard enzymatic methods (ABX Pentra 400 autoanalyzer, Horiba ABX, Montpellier, France). Plasma glycerol was analyzed with an enzymatic assay (Enzytec Glycerol, Roche Biopharm, Basel, Switzerland) automated on a Cobas Fara spectrophotometric autoanalyzer. Plasma insulin, leptin, and adiponectin concentrations were analyzed with commercially available radioimmunoassay (RIA) kits (Human insulin specific RIA, human leptin RIA, human adiponectin RIA, Millipore Corporation, Billerica, MA). Plasma concentrations of IL‐6 (Meso Scale Discovery, Gaithersburg, MD), apelin‐12 (Phoenix pharmaceuticals Inc., Burlingame, CA), RBP4 (R&D systems, Minneapolis, MN) and Vaspin (Adipogen, San Diego, IL) were determined by ELISA. The apelin‐12 ELISA has 100% cross‐reactivity with human apelin‐12, apelin‐13, and apelin‐36. Serum ACE activity was measured with a standard enzymatic assay (Bühlmann, Basel, Switzerland) while serum nesfatin‐1 (Phoenix pharmaceuticals Inc., Burlingame, CA) concentrations were measured by ELISA.
Human insulin specific ria
Manufactured by Merck Group
The Human Insulin specific RIA is a laboratory equipment product that is used for the quantitative determination of human insulin levels in biological samples. It functions as a radioimmunoassay (RIA) for the specific detection and measurement of human insulin.
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Lab products found in correlation
4 protocols using human insulin specific ria
1
Biomarker Analysis in Blood Samples
2
Plasma Metabolite and Cytokine Analysis
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Plasma glucose, NEFA, and TG concentrations were analyzed using enzymatic assays on an automated spectrophotometer (ABX Pentra 400 autoanalyzer, Horiba ANX). Plasma insulin concentrations were determined with RIA kits (Human Insulin specific RIA, Millipore Corporation). Plasma IL-6 concentrations were measured using an ELISA kit (Human ProInflammatory IL-6, Thermofischer, Bender MedSystems GmbG). Plasma amino acid concentrations and enrichments were determined by GC-MS analysis (Agilent 7890A GC/5975C; MSD) as described in detail previously (27 (link)).
3
Biochemical Analysis of Blood Samples
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Biochemical analysis of blood samples was performed as described previously (13 (link)). In short, blood samples were collected into EDTA and serum tubes and were centrifuged for 15 minutes at 1000g at 4 °C and 21 °C, respectively. Aliquots were immediately frozen in liquid nitrogen and subsequently stored at –80 °C until analysis. Plasma glucose, FFAs, TGs, and total cholesterol were analyzed with standard enzymatic methods (ABX Pentra 400 autoanalyzer, Horiba ABX). Plasma insulin concentrations were analyzed with commercially available radioimmunoassay (RIA) kits (Human insulin–specific RIA, Millipore Corp, Billerica, Millipore catalog No. EZHI-14K, RRID: AB_2800327 ). Plasma concentrations of IL-6 (Meso Scale Discovery, catalog No. K151B3S, RRID: AB_2909464 ), RBP4 (R&D Systems, catalog No. DRB400, RRID: AB_2813876 ) were determined by enzyme-linked immunosorbent assay. Serum ACE activity was measured with a standard enzymatic assay (Bühlmann, catalog No. KK-ACK).
4
Plasma Metabolic Profile Determination
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Plasma insulin concentrations were determined by commercially available radioimmunoassay kits (Human Insulin specific RIA, Millipore Corporation, MA). Plasma glucose, free fatty acids (FFAs), total cholesterol and high-density lipoproteins were measured with enzymatic assays on an automated spectrophotometer (ABX Pentra 400 autoanalyzer, Horiba ABX, Montpellier, France). Low-density lipoprotein cholesterol was calculated using the Friedewald formula [Citation18]. All blood analyses were performed at MUMC+.
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