Mouse anti-BCAS1 (Santa Cruz, 1:500, ICC and IHC, RRID: AB_10839529), mouse anti-βIII (BioLegend, 1:1000, ICC, RRID:AB_10063408), rabbit anti-βIII (BioLegend, 1:2000, RRID: AB_2564645), sheep anti-BrdU (Abcam, 1:1000, ICC, RRID:AB_302659), rabbit anti-CC3 (Millipore, 1:500, ICC, RRID: AB_91556), rabbit anti-GFAP (Dako, 1:1000, ICC, RRID: AB_10013382), rat anti-GFAP (Thermo Fisher, 1;1000, RRID:AB_2532994), chicken anti-eGFP (Abcam, 1:1000, IHC, RRID: AB_300798), mouse anti-Ki67 (BD Pharmingen, 1:300, ICC, RRID: AB_396287), rat anti-MBP (a. a. 82-87) (Millipore, 1:500, ICC, RRID: AB_94975), goat anti-PDGFRα (R&D Systems, 1:400 for IHC, 1:300 for ICC, RRID: AB_2236897), rabbit anti-PDGFRa (Santa Cruz, 1:300 for IHC, RRID: AB_631064), rabbit anti-PLP (Abcam, 1:500, ICC, RRID: AB_776593), rabbit anti-SOX2 (Cell Signalling, 1:2000, ICC, RRID: AB_2194037), goat anti-SOX2 (R&D Systems, 1:1000, IHC, RRID: AB_355110). Fluorescently labeled highly cross-absorbed secondary antibodies were purchased from Jackson ImmunoResearch and used at 1:1000 dilution. If MOM kit was used, Cy3-, DTAF-, or Cy5 conjugated streptavidin (Jackson ImmunoResearch) were used at 1:1000 dilution.
Rabbit anti pdgfra
Manufactured by Santa Cruz Biotechnology
Rabbit anti-PDGFRa is a primary antibody that recognizes the platelet-derived growth factor receptor alpha (PDGFRa) protein. It is a tool used in research applications to detect and study the expression and localization of PDGFRa in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Rat anti gfap, by Thermo Fisher Scientific (1 mentions)
Rat anti mbp, by Merck Group (1 mentions)
Goat anti sox2, by R&D Systems (1 mentions)
Mouse anti ki67, by BD (1 mentions)
Rabbit anti gfap, by Agilent Technologies (1 mentions)
Goat anti pdgfrα, by R&D Systems (1 mentions)
Chicken anti egfp, by Abcam (1 mentions)
Rabbit anti sox2, by Cell Signaling Technology (1 mentions)
Cy5 conjugated streptavidin, by Jackson ImmunoResearch (1 mentions)
Sheep anti brdu, by Abcam (1 mentions)
Rabbit anti plp, by Abcam (1 mentions)
Goat anti sox10, by R&D Systems (1 mentions)
2 protocols using rabbit anti pdgfra
1
Multiplex Immunostaining Protocol for Neural Lineage Analysis
2
Immunocytochemical Characterization of OPCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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OPCs were cultured on poly-L-lysine coated coverslips. Cells were fixed for 10 min in 4 % paraformaldehyde and processed for Immunocytochemistry (ICC) following a standard protocol. Coverslips were rinsed 5 x 6 min in PBS and blocked with 3 % BSA, 0.1 % Triton and 10 % horse serum in PBS for 1 hour. They were then incubated O/N at 4 °C in blocking solution with the following primary antibodies: Mouse anti-APC (Millipore OP80-100UG), Goat anti-β-galactosidase (CAPPEL 56028), Rabbit anti-PDGFRa (Santa Cruz sc-338), Mouse anti-Sox10 (Sigma SAB1402361), Goat anti-Sox10 (R&D systems AF2864). Slides were washed in PBS for 30 min and incubated with the appropriate fluorescence labelled secondary antibodies 1hour at RT. DAPI was used to stain the nuclei.
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