Monomers, methacrylic acid (MAA), 2-(dimethylamino)ethyl methacrylate (DMAEMA), oligo(ethylene glycol) methacrylate (OEGMA) (average Mn = 475 g/mol), benzyl methacrylate (BzMA) and cross-linking agent ethylene glycol dimethacrylate (EGDMA, difunctional monomer) were purified by passing them through a column packed with inhibitor-removing resins 311,340 and 311,332 from Sigma Aldrich (Athens, Greece). 2,2-azobis(isobutyronitrile) (AIBN) was recrystallized from methanol and used as the radical initiator. 1,4-dioxane (99.8% pure) was chosen as the solvent of the reaction and dried using molecular sieves. 4-cyano-4-(phenylcarbonothioylthio) pentanoic acid (CPAD), i.e., the chain transfer agent of the reaction, pyrene and deuterated dimethyl sulfoxide-d6 (dDMSO) were used as received (all from Sigma-Aldrich, Athens, Greece). Dialysis tubing membranes (MEMBRA-CELL) from regenerated cellulose of MWCO 3500 with a diameter of 22 mm were obtained from SERVA (Heidelberg, Germany).
4 cyano 4 phenylcarbonothioylthio pentanoic acid cpad
Manufactured by Merck Group
Sourced in Greece
4-cyano-4-(phenylcarbonothioylthio) pentanoic acid (CPAD) is a chemical compound used in laboratory settings. It is a white crystalline solid with a specific chemical structure and formula. CPAD is commonly used as a reagent or intermediate in various chemical reactions and analyses, but no further details on its intended use or applications can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
3 protocols using 4 cyano 4 phenylcarbonothioylthio pentanoic acid cpad
1
Synthesis of Functional Polymer Nanoparticles
2
Thiol-PNA probe for SARS-CoV-2, H1N1, and MERS-CoV
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The thiol-terminated PNA probe and the short gene fragments with the following sequences were obtained from biomers.net (Germany):
Thiol-terminated PNA probe: (5′-thiotic acid–8-Amino-3,6-dioxaoctanoic acid GCATCTCCTGATGAGGTTCCACCTG-3′), SARS-CoV-2 RdRP: 5′-CAGGT GGAAC CTCATCAGGA GATGC-3′ [13 (link)],
H1N1: 5′-CTAGTACTGTGTCTACACAGTGTC-3′ [16 ],
MERS-CoV: 5′-CGATTATGTGAAGAG-3′ [6 (link)].
Potassium chloride (KCl), calcium chloride, sodium chloride (NaCl), sodium nitrate (NaNO3), magnesium chloride (MgCl2), and sodium dihydrogen phosphate (NaH2PO4) were purchased from Cymit Química (Spain). 1-Hexanethiol (HT), graphite powder, zirconium dichloride oxide octahydrate (ZrOCl2), ferrocenylmethyl methacrylate (FcMMA), 4-cyano-4-(phenylcarbonothioylthio)-pentanoic acid (CPAD), 2,2′-azobis [2-(2-imidazolin- 2-yl)propane] dihydrochloride (AIPH), ethanol (EtOH), N,N-dimethylformamide (DMF), sodium tetrachloroaurate (III) dehydrate (NaAuCl4), potassium permanganate (KMnO4), hydrochloric acid (HCl), potassium hydroxide (KOH), hydrogen peroxide (H2O2), (30%), potassium ferricyanide (K3 [Fe(CN)6]), potassium ferrocyanide (K4 [Fe(CN)6]), from Sigma-Merck (USA). Disposable well cells and carbon screen printed electrodes (CSPE, Ref 110) were purchased from Metrohm-DropSens (Spain).
Thiol-terminated PNA probe: (5′-thiotic acid–8-Amino-3,6-dioxaoctanoic acid GCATCTCCTGATGAGGTTCCACCTG-3′), SARS-CoV-2 RdRP: 5′-CAGGT GGAAC CTCATCAGGA GATGC-3′ [13 (link)],
H1N1: 5′-CTAGTACTGTGTCTACACAGTGTC-3′ [16 ],
MERS-CoV: 5′-CGATTATGTGAAGAG-3′ [6 (link)].
Potassium chloride (KCl), calcium chloride, sodium chloride (NaCl), sodium nitrate (NaNO3), magnesium chloride (MgCl2), and sodium dihydrogen phosphate (NaH2PO4) were purchased from Cymit Química (Spain). 1-Hexanethiol (HT), graphite powder, zirconium dichloride oxide octahydrate (ZrOCl2), ferrocenylmethyl methacrylate (FcMMA), 4-cyano-4-(phenylcarbonothioylthio)-pentanoic acid (CPAD), 2,2′-azobis [2-(2-imidazolin- 2-yl)propane] dihydrochloride (AIPH), ethanol (EtOH), N,N-dimethylformamide (DMF), sodium tetrachloroaurate (III) dehydrate (NaAuCl4), potassium permanganate (KMnO4), hydrochloric acid (HCl), potassium hydroxide (KOH), hydrogen peroxide (H2O2), (30%), potassium ferricyanide (K3 [Fe(CN)6]), potassium ferrocyanide (K4 [Fe(CN)6]), from Sigma-Merck (USA). Disposable well cells and carbon screen printed electrodes (CSPE, Ref 110) were purchased from Metrohm-DropSens (Spain).
3
Anionic Polymerization of Methacrylate Monomers
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Monomers, di(ethylene glycol) methyl ether methacrylate (DEGMA), 2-(dimethylamino)ethyl methacrylate (DMAEMA), and oligo(ethylene glycol) methacrylate (OEGMA) (average Mn = 475 g/mol) were purified, passing through a column packed with inhibitor removing resins 311,340 and 311,332 from Sigma-Aldrich (Athens, Greece). 2,2-azobis(isobutyronitrile) (AIBN) was recrystallized from methanol and was used as the radical initiator. 1,4-dioxane (99.8% pure) was chosen as the solvent of the reaction and was dried using molecular sieves. 4-cyano-4-(phenylcarbonothioylthio) pentanoic acid (CPAD) was the chain transfer agent of the reaction, and n-hexane, tetrahydrofuran (THF), sodium sulfate (Na2SO4), pyrene, and deuterated chloroform (CDCl3) were used as received (all from Sigma-Aldrich).
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