Sa00001 3

Manufactured by Proteintech

Sourced in China, United States

SA00001-3 is a laboratory equipment product. It serves as a core function without further interpretation or extrapolation on its intended use.

Automatically generated - may contain errors

Lab products found in correlation

Bs13278, by Bioworld Technology (2 mentions) Sa00001 1, by Proteintech (2 mentions) Proteinase inhibitor, by Roche (2 mentions) Phosphatase inhibitor, by Roche (2 mentions) Ab6658, by Abcam (1 mentions) Ab125096, by Abcam (1 mentions) Sa00001 8, by Proteintech (1 mentions) Supersignal west femto, by Thermo Fisher Scientific (1 mentions) P38 mapk, by Affinity Biosciences (1 mentions) P p38 mapk, by Affinity Biosciences (1 mentions) Gapdh 60004 1 ig, by Proteintech (1 mentions) Af5395, by Affinity Biosciences (1 mentions) Af5450, by Affinity Biosciences (1 mentions) Af4001, by Affinity Biosciences (1 mentions) Nb100 2828, by Novus Biologicals (1 mentions) Af6456, by Affinity Biosciences (1 mentions) Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions) Af360, by R&D Systems (1 mentions) Goat anti rabbit igg, by Santa Cruz Biotechnology (1 mentions) Sc 17781, by Santa Cruz Biotechnology (1 mentions)

4 protocols using sa00001 3

Western Blot Analysis of GFP and mCherry

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Use 6% SDS-PAGE gel, and the separated proteins were transferred by electro blotting to NC membranes. The membranes were blocked with 5% non-fat dry milk in TBST and incubated with the primary antibody including anti-GFP antibody (Abcam, ab6658) and anti-mCherry antibody (Abcam, ab125096) overnight at 4 °C. Then washing three times, the second antibody including anti-Goat HRP (Proteintech, SA00001-3) and anti-mouse HRP (Proteintech, SA00001-8) for about 2 h Immunolabelling was detected using SuperSignal West Femto (Thermo Fisher Scientific, 34096).

Zhao S., Mo G., Wang Q., Xu J., Yu S., Huang Z., Liu W, & Zhang W. (2024). Role of RB1 in neurodegenerative diseases: inhibition of post-mitotic neuronal apoptosis via Kmt5b. Cell Death Discovery, 10, 182.

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Immunoblotting Analysis of Adipogenic Protein Signaling

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Total proteins were extracted using RIPA-lysis buffer containing proteinase inhibitors (No. 04693159001; Roche) and phosphatase inhibitors (No. 04906845001; Roche), separated by SDS-PAGE, transferred to PVDF membranes, and analyzed by immunoblotting. Primary antibodies against the following proteins were used: TH (66334-1-Ig; Proteintech), PKA (AF5450; Affinity), p-HSL (AF8026; Affinity), HSL (AF6403; Affinity), p-p38 MAPK (AF4001; Affinity), p38 MAPK (AF6456; Affinity), PGC1α (AF5395; Affinity), UCP1 (NB100-2828; Novus Biologicals), and GAPDH (60004-1-Ig; Proteintech). The antibodies were diluted 1:1000 with PBS. The membranes were then incubated with a peroxidase-conjugated secondary antibody (BS13278 [Bioworld], SA00001-1 [Proteintech], SA00001-3 [Proteintech]), and antibody-specific signals were detected by enhanced chemiluminescence and quantified using an automatic chemiluminescence imaging system (Complex 2000; Nanjing PuoAoXin Biotechnology).

Zhou H., Wang Z., Chen C., Hu H., Jiang B., Yin Y., Zhang K., Shen M, & Wu S. (2023). Effect of High-Intensity Interval Training on Fatty Infiltration After Delayed Rotator Cuff Repair in a Mouse Model. Orthopaedic Journal of Sports Medicine, 11(5), 23259671231170192.

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Protein Expression Analysis by Western Blot

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Total proteins were extracted using radioimmunoprecipitation assay lysis buffer containing proteinase inhibitors (No. 04693159001, Roche Diagnostics, Switzerland), and phosphatase inhibitors (No. 04906845001, Roche), separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene difluoride (PVDF) membranes, and analyzed by immunoblotting. Primary antibodies against the following proteins were used: TH (66334-1-Ig, Proteintech), protein kinase A (PKA) (AF5450, Affinity (China)), p-HSL (AF8026, Affinity), hormone-sensitive lipase (HSL) (AF6403, Affinity), p-p38 mitogen-activated protein kinase (MAPK) (AF4001, Affinity), p38 MAPK (AF6456, Affinity), PGC1α (AF5395, Affinity), UCP1 (NB100-2828, Novus Biologicals), and GAPDH (60004-1-Ig, Proteintech). The antibodies were diluted 1:1,000 with PBS. The membranes were then incubated with a peroxidase-conjugated secondary antibody (BS13278, Bioworld (China); SA00001-1, Proteintech; SA00001-3, Proteintech), and antibody-specific signals were detected by enhanced chemiluminescence and quantified using the Automatic Chemiluminescence Imaging System (Complex 2000; Nanjing PuoAoXin Biotechnology, China).

Zhou H., Chen C., Hu H., Jiang B., Yin Y., Zhang K., Shen M., Wu S, & Wang Z. (2023). High-intensity interval training improves fatty infiltration in the rotator cuff through the β3 adrenergic receptor in mice. Bone & Joint Research, 12(8), 455-466.

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Western Blot Analysis of ABCB1 and Related Signaling

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For sample preparation, when the cell culture reached 80% confluence, the cells were collected and split for 40 minutes on ice, the supernatant was denatured in 5x loading buffer containing SDS in boiling water for 10 minutes. We suggest not to boil the sample after lysis when preparing protein samples for ABCB1 determination by western blot. Next, 5% bovine serum albumin (BSA) dissolved in TBS containing 0.1% Tween-20 (TBST) was used for blocking and antibody dilution. The following antibodies and dilutions were used: actin (1:1,000, HC201, TransGen), CCL20 (1:1,000, AF360, R&D), p65 (1:1,000, 8242S), p-p65 (1:1,000, 3033S), p38 (1:1,000, 9212), p-p38 (1:1,000, 4631), p-PKCζ (1:1,000, 9378), ABCB1 (1:1,000, 13342S) (all from Cell Signaling Technology, USA), PKCζ (1:100, sc-17781, Santa Cruz), goat anti-mouse IgG-HRP (1:5,000, sc-2005, Santa Cruz), goat anti-rabbit IgG (1:5,000, sc-2004, Santa Cruz), donkey anti-goat IgG-HRP (1:2,000, SA00001-3, Proteintech). Western HRP Substrate (WBLUF0500, Millipore) was used to detect horseradish peroxidase–conjugated secondary antibodies.

Chen W., Qin Y., Wang D., Zhou L., Liu Y., Chen S., Yin L., Xiao Y., Yao X.H., Yang X., Ma W., Chen W., He X., Zhang L., Yang Q., Bian X., Shao Z.M, & Liu S. (2018). CCL20 triggered by chemotherapy hinders the therapeutic efficacy of breast cancer. PLoS Biology, 16(7), e2005869.

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