Integrin α2

Antibodies against Vimentin, and αSMA were purchased from Sigma-Aldrich. N-cadherin, p44/42 MAPK, Phospho-p44/42 MAPK, EGFR antibodies were from Cell Signaling Technology (Danvers, MA); α-tubulin and ROBO1 from Abcam (Cambridge, MA). Fibronectin, Integrin αV, Integrin α2, Integrin α5, Integrin β1, Integrin β4 (BD Biosciences); MMP14 (Epitomics, Burlingam, CA); Ki67 Vector Laboratories (Burlingame, CA).

Cells were lysed in radioimmune precipitation assay (RIPA) buffer as described before [5 (link)]. Protein was subjected to either 8% Tris-glycine or 4–12% Bis/Tris (Invitrogen) SDS-PAGE. Quantitation of band intensity was performed with ImageJ software v1.47f (http://rsb.info.nih.gov/ij/). The following antibodies were used in this study: RAD9, integrin β1, integrin α2, integrin α5, PARP-1 monoclonal antibodies (BD Biosciences), β-actin, and α-tubulin monoclonal antibodies (Sigma).
To isolate the nuclear fraction, cells from three 100-mm culture dishes were fractionated using a NE-PER nuclear and cytoplasmic extraction kit (Thermo Scientific), following the manufacturer’s instructions. PARP-1 and α-tubulin were used as nuclear and cytosolic markers respectively.