The cDNA fragment of KLF9 3′-UTR that contained the wild-type or mutant miR-600 binding site was inserted into the miRNA reporter vector (Promega, Madison, WI). Briefly, miR-600 knockdown ovarian cancer cells and control cells were transfected with pMIR-reporter luciferase vector containing a specific sequence of wild-type or mutant KLF9 fragment, using siRNA transfection (Invitrogen, NY, USA). Cells were collected and lysed for luciferase detection 48 h after transfection. The relative luciferase activity was normalized against to the Renilla luciferase activity [19 ].
Mirna reporter vector
Manufactured by Promega
The MiRNA reporter vector is a tool designed for the study of microRNA (miRNA) regulation. It contains a reporter gene that is under the control of a miRNA response element, allowing for the assessment of miRNA activity in a cell-based system. The core function of this product is to provide a platform for investigating miRNA-mediated gene expression.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mirna reporter vector
1
Investigating miR-600 Regulation of KLF9 3'UTR
2
Luciferase Assay for miR-552 Targeting
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The cDNA fragment of MUC15 3'-UTR that contained the wild-type or mutant miR-552 binding site was inserted into the miRNA reporter vector (Promega, Madison, WI). Briefly, cervical cancer cells were co-transfected with miR-552 sponge, miR-552 mimic or miR-control and pMIR-reporter luciferase vector containing a specific sequence of wild-type or mutant MUC15 fragment, using siRNA transfection (Invitrogen, NY, USA). Cells were collected and lysed for luciferase detection 48 h after transfection. The relative luciferase activity was normalized against to the Renilla luciferase activity 19 (link).
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