The concentrations of fructose, glucose, maltose, and sucrose were measured by high-performance anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD) with internal standardization, as described before (De Roos et al., 2018 (link)). Briefly, an ICS3000 chromatograph (Dionex, Sunnyvale, CA, United States) equipped with a CarbopacTM PA10 column (Dionex) and coupled to a pulsed amperometric detector (Dionex) was used. The same mobile phase and eluent gradient were applied. All samples were deproteinized, vortexed, centrifuged (21,912 × g, 15 min, 4°C), and filtered (0.2-μm pore-size Whatman filters; GE Healthcare Life Sciences, Bucks, United Kingdom) before injection (10 μl) into the column.
Ics3000 chromatograph
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ICS3000 is a chromatograph system designed for high-performance ion chromatography. It provides precise and reliable separation and detection of ionic compounds.
Automatically generated - may contain errors
3 protocols using ics3000 chromatograph
1
HPAEC-PAD Carbohydrate Analysis
2
HPAEC-PAD Analysis of Maltooligosaccharides
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The concentrations of maltotriose, maltotetraose, maltopentaose, maltohexaose, and maltoheptaose were measured by HPAEC-PAD with internal standardization, as described before for maltotriose (De Roos et al., 2018 (link)). Briefly, an ICS3000 chromatograph (Dionex) equipped with a CarbopacTM PA100 column (Dionex) and coupled to a pulsed amperometric detector (Dionex) was used. The same mobile phase and eluent gradient were applied. All samples and standards were deproteinized, vortexed, centrifuged, and filtered, as described above, before injection (10 μl) into the column.
3
Glycan Fingerprinting by HPAEC-PAD
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ProDigest (Ghent, Belgium) by high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using a ICS-3000 chromatograph (Dionex, Sunnyvale, CA, USA) equipped with a CarboPacPA20 column (Dionex), as described previously 50 . Sample preparation involved initial dilution of the sample with ultrapure water followed by deproteinization with acetonitrile (1:1), centrifugation (21,380 × g, 10 min) and filtration (0.2 μm PTFE, 13 mm syringe filter, VWR International) prior to injection (5 μL) into the column. Qualitative glycan fingerprints were obtained by plotting the detected signal (nC) against the elution time.
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