Cytarabine ara c

The AML cell lines U937, MOLM-14, MV4-11, HL60, HEL, THP-1, NOMO-1, OCI-AML2, OCI-AML3 and NB4 were provided by collaborators or were purchased from ATCC or DSMZ (Braunschweig, Germany). All cell lines were cultured in RPMI-1640 medium containing 10% FBS (GIBCO, Life Technologies, Carlsbad, CA, USA), 2 μM l−1 glutamine, 100 U mL−1 penicillin, and 100 μg ml−1 streptomycin (GIBCO, Life Technologies, Carlsbad, CA, USA). The 293T cell line was purchased from ATCC and cultured in DMEM containing 10% FBS (GIBCO, Life Technologies, Carlsbad, CA, USA), 2 μM l−1 glutamine, 100 U mL−1 penicillin, and 100 μg mL−1 streptomycin (GIBCO, Life Technologies, Carlsbad, CA, USA). Daunorubicin (DNR) and cytarabine (ARA-C) were purchased from Selleck Chemicals LLC (Houston, TX, USA) and Sigma-Aldrich (St. Louis, MO, USA), respectively; SIRT6 chemical inhibitor [2,4-dioxo-N-(4-(pyridin-3-yloxyphenyl)-1,2,3,4-tetrahydroquinazoline-6-sulfonamide, henceforth named compound 1] was obtained from MolPort (Riga, Latvia).

Gemcitabine (purity > 98%), cycloheximide (purity > 93%) and MTT (purity > 98%) were purchased from Sigma-Aldrich (St. Louis, USA). LY294002 (purity > 98%) was purchased from Beyotime Institute of Biotechnology (Shanghai, China). Digoxin (purity > 97%), etoposide (purity > 99%), paclitaxel (purity > 99%), cisplatin (purity > 99%), 5-Fluorouracil (5-FU, purity > 99%), cytarabine (ara-C, purity > 99%), doxorubicin (purity > 99%) and MG132 (purity > 97%) were purchased from Selleck Chemicals (Houston, USA). Actinomycin D (purity > 95%) was purchased from KeyGen (Nanjing, China). Anti-NQO1, anti-HO-1 and anti-GCLC antibodies were obtained from Santa Cruz Biotechnology (Texas, USA). Anti-Keap1, anti-Nrf2, anti-p-Akt, anti-Akt, anti-p-P38 and anti-P38, anti-p-ERK1/2, anti-ERK1/2, anti-p-JNK and anti-JNK antibodies were purchased from Cell Signaling Technology (Danvers, USA). Anti-ABCC1 and anti-ABCC5 antibodies were purchased from ABclonal (Wuhan, China). Anti-β-actin and anti-ubiquitin antibodies were obtained from Bioworld (Minnesota, USA). Anti-lamin A antibody was obtained from Sigma-Aldrich (St. Louis, USA).