All images were obtained at 72 hpf when the live embryos were washed with 1×E3 and mounted in 3% methylcellulose. Whole body images were obtained at 10× on Nikon 80i compound microscope (Nikon Instruments). High magnification images of somites 11-13 were obtained at 20× using confocal microscope (Nikon A1R Si Confocal Eclipse Ti series). All confocal and software settings were kept unchanged during image acquisition. Images were processed with an NIS-Elements advanced research image acquisition and analysis system (Nikon Instruments), using the maximum intensity projection feature applied to z-stacks for greater depth of clarity. Image files were exported as TIFF files. Figures were composed with Adobe Photoshop CS6 (Adobe, USA).
A1r si confocal eclipse ti series
Manufactured by Nikon
The A1R Si Confocal Eclipse Ti series is a high-performance confocal microscope system designed for advanced imaging applications. It features a high-resolution, high-speed confocal scanner and a high-sensitivity detector that enables efficient fluorescence imaging.
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3 protocols using a1r si confocal eclipse ti series
1
Zebrafish Whole-Body Imaging at 72 hpf
2
Imaging Wildtype and Talin Mutant Larvae
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WT; mylz2:mCherry and Tln1-/-; mylz2:mCherry embryos at 4 dpf were imaged at 10× on the confocal microscope (Nikon A1R Si Confocal Eclipse Ti series).
3
Imaging Embryonic Tissue Development
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Embryos were mounted in 95% glycerol in 1× PBS with Tween 20 and imaged at 10× on Nikon 80i compound microscope (Nikon Instruments, Melville, NY) for WISH and cartilage staining experiments. The palate and mandible were dissected and flat mounted before imaging them at 40×. Embryos were mounted in 3% methylcellulose and imaged at 20× on the confocal microscope (Nikon A1R Si Confocal Eclipse Ti series) for cell proliferation, migration, and apoptosis assays. All images were processed with NIS-Elements advanced image acquisition and analysis system (Nikon Instruments). Figures were composed with Adobe Photoshop CS6 and Adobe Illustrator CS6 (Adobe, San Jose, CA). All measurements were performed using ImageJ (NIH).
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