L arginine monohydrochloride

Manufactured by Merck Group

Sourced in United States

L-arginine monohydrochloride is a chemical compound that is commonly used in laboratory settings. It is a salt of the amino acid L-arginine and hydrochloric acid. This compound is a white, crystalline powder that is soluble in water. It is a commonly used reagent in various biochemical and biological applications.

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L-arginine (442 citations)

22 protocols using l arginine monohydrochloride

SILAC-based Proteome Analysis of Torin1 Treatment

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car2::NAT lys1-131 arg3-d4 cells were inoculated in YES medium overnight and then washed into EMM-G containing 75 mg/l of either light [L-arginine monohydrochloride (Sigma) and L-lysine monohydrochloride (Sigma)] or medium [lysine-L, 2HCl 4.4.5.5-D4 (Cat code DLM-2640, Eurisotop), arginine-L, HCl, U-13C6 99%13C (cat. no. CLM-2265, Eurisotop)] amino acids. Cells were cultured in log phase for 48 h to ensure complete incorporation of labelled amino acids into the proteome. Light-labelled cultures were treated with DMSO and medium-labelled cultures were treated with a final concentration of 25 µM Torin1 at a density of 2.04×10⁶ cells/ml. Approximately 4.8×10⁹ cells were harvested for each sample. After 30 min cultures were harvested by centrifugation (3000 g for 5 min), washed in 20 ml of STOP buffer (10 mM EDTA, 1 mM sodium azide, 50 mM sodium fluoride, 0.9% NaCl), followed by washing with 10 ml of ice cold ddH₂O. The final pellets were then resuspended in an appropriate volume of ice cold ddH₂O and dropped directly into liquid nitrogen to produce frozen cell droplets.

Baker K., Kirkham S., Halova L., Atkin J., Franz-Wachtel M., Cobley D., Krug K., Maček B., Mulvihill D.P, & Petersen J. (2016). TOR complex 2 localises to the cytokinetic actomyosin ring and controls the fidelity of cytokinesis. Journal of Cell Science, 129(13), 2613-2624.

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Isolation and Characterization of Propolis Compounds

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Silver nitrate (≥99%), sodium hydroxide (NaOH), bovine serum albumin (BSA), HPLC grade m ethanol, ethanol, formic acid, fetal bovine serum, Mueller–Hinton, Luria–Bertani, and Dulbecco’s Modified Eagle media, as well as L-asparagine (98%), L-arginine monohydrochloride (≥98%), L-glutamine solution (200 mM), sodium pyruvate solution (100 mM), and penicillin-streptomycin solution (1000 U/1 U per mL) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Pinocembrin (≥95%), pinobanksin-3-O-acetate (≥95%) (Pb-3-O-Ac), chrysin (≥95%), galangin (≥95%), and quercetin (≥95%) were purified from propolis collected in Ures, Sonora, Mexico (N 2927.1810, W 110 23.398) by chromatographic isolation procedures over silica gel 60 (0.015–0.040 mm; Merck KGaA), using progressive proportions of ethyl acetate in hexane as the mobile phase (Tedia Company, Fairfield, OH, USA). Caffeic acid phenethyl ester (CAPE) was synthesized according to the esterification of caffeic acid and phenethyl alcohol, based on the procedure described by Grunberger et al. [16 (link)].

Mendez-Pfeiffer P., Ballesteros-Monrreal M.G., Gaona-Ochoa J., Juarez J., Gastelum-Cabrera M., Montaño-Leyva B., Arenas-Hernández M., Caporal-Hernandez L., Ortega-García J., Barrios-Villa E., Velazquez C, & Valencia D. (2022). Biosynthesis of Silver Nanoparticles Using Seasonal Samples of Sonoran Desert Propolis: Evaluation of Its Antibacterial Activity against Clinical Isolates of Multi-Drug Resistant Bacteria. Pharmaceutics, 14(9), 1853.

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Bovine Serum Albumin Characterization

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BSA (catalogue no. A7638, 99+% of purity), L-arginine monohydrochloride (Arg), L-arginine amide hydrochloride (ArgEE) and L-arginine ethyl ester hydrochloride (ArgEE) were purchased from Sigma–Aldrich and used without further purification. All solutions for the experiments were prepared using deionized water obtained with Easy-Pure II RF system (Barnstead, USA). BSA samples were prepared by dissolving solid BSA in 0.1 M phosphate buffer solutions at pH 7.0. All the experiments were performed with freshly prepared solutions of BSA. BSA concentration was determined spectrophotometrically at 280 nm using the absorption coefficient

A_{cm}^{1 %}

of 6.58^{61 (link)}.

Borzova V.A., Markossian K.A., Kleymenov S.Y, & Kurganov B.I. (2017). A change in the aggregation pathway of bovine serum albumin in the presence of arginine and its derivatives. Scientific Reports, 7, 3984.

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Caerulein and L-Arginine Induced Pancreatitis

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AP was induced to WT and miR-29a/b1–KO adult mice (6–8 weeks of age) by (a) 8 hourly i.p. injections of sterile caerulein (Sigma-Aldrich) for 2 consecutive days (50 μg/kg body weight solubilized in PBS) or by (b) 2 i.p. injections of sterile L-arginine monohydrochloride (Sigma Aldrich) prepared in PBS (8%), pH adjusted to 7.0, at a dose of 4 g/kg body weight.
Control (WT and KO) mice received an equal amount of sterile saline (PBS). caerulein/PBS-injected mice were sacrificed at different time points following the final dose: 6-hour (acute phase), 2-day or 4-day (early recovery phase), and 7-day or 10-day (late recovery phase) for serum and tissue collection. Mice injected with L-arginine/PBS were sacrificed at 72 hours after the first injection. Each group of mice consisted of 5–7 randomly selected littermates per time point/genotype/group.
Pancreatic specimens for mice fed with ethanol-rich diet and corresponding normal diet were provided by Aurelia Lugea, Cedar-Sinai Medical Center.

Dey S., Udari L.M., RiveraHernandez P., Kwon J.J., Willis B., Easler J.J., Fogel E.L., Pandol S, & Kota J. (2021). Loss of miR-29a/b1 promotes inflammation and fibrosis in acute pancreatitis. JCI Insight, 6(19), e149539.

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Auxin-Induced Proteome Profiling

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HCT116 (Ki-67-AID) were cultured in SILAC medium (McCoy’s 5A Media for SILAC, Thermo Fisher Scientific): light (L-lysine monohydrochloride (Sigma-Aldrich) l-arginine monohydrochloride (Sigma-Aldrich) (auxin) and heavy (L-lysine-13C6,15N2 hydrochloride (Sigma-Aldrich) (control) respectively for at least 6 passages. The cells were treated with doxycycline (2 μg/ml) and 2 mM thymidine, 24 and 18 h before the addition of auxin 1000 μM, respectively. After the 4 h treatment with auxin, the cells were counted and mixed 1:1 (control/auxin). Nuclear isolation was performed as described in [66 ]. Cell lysate was sent for mass spectrometry analysis.

Stamatiou K., Huguet F., Serapinas L.V., Spanos C., Rappsilber J, & Vagnarelli P. (2024). Ki-67 is necessary during DNA replication for fork protection and genome stability. Genome Biology, 25, 105.

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Comprehensive TMAO Metabolism Analysis

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TMAO (Sigma, Cat. 317594), D9-TMAO(Cambridge Isotopes,Cat. DML-4779-1), [6]-gingerol (Sigma, Cat. 345868), CDN1163 (Sigma, Cat. SML1682), MCC950 sodium (MCE, Cat. HY-12815A), Glucose (Sinopharm Chemical Reagent Co., Ltd. Cat. 10010592), GLP-1 (7-36) (MCE, Cat. HY-P0054), L-Arginine monohydrochloride (Sigma, Cat. A5131), Fatty acid free bovine serum albumin (Equitech-Bio, Inc.Cat. BAH66), Collagenase from Clostridium histolytium (Sigma, Cat. C9263), Human recombinant insulin (Lilly Cat. HI0219), Fura-2, AM (Invitrogen, Cat. F1221), mitoSOX Red, Molecular Probes (Invitrogen, M36008), phosphoenolpyruvate (Sigma, Cat. P7127), ATP disodium salt (Sigma, Cat. A3377), pyruvate kinase (Sigma, P1903-1KU), lactate dehydrogenase (Sigma, Cat. 59747), NADH (Sigma, Cat. N8129), digitonin (Sigma, Cat. D141), protease inhibitor cocktail (Sigma, Cat. P8340), PMSF (Roche, Cat. 10837091001), 4-Bromo A23187 (MCE, Cat. HY-N6694), Anti-Biotin MicroBeads (Miltenyi Biotec, Cat. 130-090-485).

Kong L., Zhao Q., Jiang X., Hu J., Jiang Q., Sheng L., Peng X., Wang S., Chen Y., Wan Y., Hou S., Liu X., Ma C., Li Y., Quan L., Chen L., Cui B, & Li P. (2024). Trimethylamine N-oxide impairs β-cell function and glucose tolerance. Nature Communications, 15, 2526.

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Isolation of Phb from Rabbit Muscles

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Hepes and L-arginine monohydrochloride were purchased from “Sigma-Aldrich” (St. Louis, MO, USA), 1,4-dithiothreitol (DTT) was purchased from PanReac (Barcelona, Spain), NaCl was purchased from “Reakhim” (Moscow, Russia). The procedure of isolation of Phb from rabbit skeletal muscles was described in [54 (link)].

Mikhaylova V.V., Eronina T.B., Chebotareva N.A., Shubin V.V., Kalacheva D.I, & Kurganov B.I. (2020). Effect of Arginine on Chaperone-Like Activity of HspB6 and Monomeric 14-3-3ζ. International Journal of Molecular Sciences, 21(6), 2039.

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Arginine Compound Procurement Protocol

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In this study, L-Arginine monohydrochloride, N-nitro-L-arginine methyl ester monohydrochloride, D-Arginine monohydrochloride were procured from Sigma whereas gentamicin sulphate was procured from Schering-Plough.

Başhan İ., Başhan P., Seçilmiş M.A, & Şingirik E. (2014). Protective effect of L-arginine on gentamicin-induced nephrotoxicity in rats. Indian Journal of Pharmacology, 46(6), 608-612.

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Measurement of MDSC-mediated T cell suppression

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CD66+ MDSC were separated from PBMC as per kit instructions using magnetic bead isolation (EasySep™) of PE-conjugated anti-CD66 (Miltenyi Biotec). CD66− PBMC were cultured at 37°C, 5% CO₂, overnight either alone or with CD66+ MDSC added back at a 1:1 ratio in custom arginine-low media: SILAC media (Sigma Aldrich) plus filter-sterilized L-arginine monohydrochloride (0.115mM, Sigma Aldrich) and filter-sterilized L-lysine hydrochloride (0.2186mM, Sigma Aldrich), 15% human serum (Sigma Aldrich), Penicillin-Streptomycin-Glutamine (0.5mg/ml, Thermo Fisher). Cells were stimulated with 1ug/ml 15mer SIVmac239 Gag peptide pool (NIH AIDS Reagent Program) or 0.4ug/ml superantigen SEB⁷ from Staphylococcus aureus (Sigma Aldrich) for 4 days. Duplicate control un-stimulated wells for each cell mixture (PBMC alone, PBMC with CD66+MDSC) were included for background proliferation subtraction. Proliferation was measured by ICS on day 4 post-stimulation using CD8 clone SK1 PECy7 (Biolegend), Ki67 clone B56 Alexa Flour 488 (Becton Dickinson), CD3 clone SP34-2 APC (Becton Dickinson). CD66+ MDSC-mediated suppression was measured by comparing the proliferation stimulated in the absence of CD66+ cells with the proliferation stimulated in the presence of CD66+ cells. Replicates were included for all PBMC wells, and PBMC + MDSC when MDSC yield allowed, and averaged.

Dross S.E., Munson P.V., Kim S.E., Bratt D.L., Tunggal H.C., Gervassi A.L., Fuller D.H, & Horton H. (2016). Kinetics of myeloid derived suppressor cell frequency and function during SIV infection, combination antiretroviral therapy and treatment interruption. Journal of immunology (Baltimore, Md. : 1950), 198(2), 757-766.

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Vasoactive Compound Comparison Study

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Rasilez^¯ (Aliskiren; Novartis, Italy), l-phenylephrine hydrochloride,
L-NAME, apocynin, SOD, acetylcholine chloride, sodium pentobarbital, losartan,
superoxide dismutase, sodium nitroprusside and L-arginine monohydrochloride were
purchased from Sigma-Aldrich (USA). The salts and reagents used were of analytical
grade and purchased from Sigma-Aldrich and Merck (Germany).

Santuzzi C.H., Tiradentes R.V., Mengal V., Claudio E.R., Mauad H., Gouvea S.A, & Abreu G.R. (2014). Combined aliskiren and L-arginine treatment has antihypertensive effects and prevents vascular endothelial dysfunction in a model of renovascular hypertension. Brazilian Journal of Medical and Biological Research, 48(1), 65-76.

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