Mmp 3

Manufactured by R&D Systems

Sourced in United States, United Kingdom

MMP-3 is a matrix metalloproteinase enzyme that is involved in the breakdown of extracellular matrix components. It plays a role in various physiological and pathological processes.

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Lab products found in correlation

Mmp 1, by R&D Systems (8 mentions) Mmp 9, by R&D Systems (7 mentions) Interleukin 6 (il 6), by R&D Systems (6 mentions) Timp 1, by R&D Systems (4 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (4 mentions) Mmp 13, by R&D Systems (3 mentions) Mmp 2, by R&D Systems (3 mentions) Gm csf, by R&D Systems (3 mentions) Mcp 1, by BioLegend (3 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (2 mentions) Nobiletin, by Cayman Chemical (2 mentions) Vascular endothelial growth factor (vegf), by R&D Systems (2 mentions) Ccl25, by R&D Systems (2 mentions) Igfbp2, by R&D Systems (2 mentions) Cd40l, by R&D Systems (2 mentions) Recombinant human il 1β, by R&D Systems (2 mentions) Tnf α, by Boster Bio (1 mentions) Interleukin 6 (il 6), by Boster Bio (1 mentions) Horseradish peroxidase conjugated goat anti human igg, by Santa Cruz Biotechnology (1 mentions) Recombinant human mmp 10, by R&D Systems (1 mentions)

Close spelling variants of this product

MMP-3 ELISA kits (4 citations) Human MMP-3 Quantikine ELISA kit (2 citations) Quantikine Human MMP-3 (2 citations) Human Total MMP-3 ELISA kits (2 citations) Anti MMP-3 antibody (2 citations)

42 protocols using mmp 3

AOPP-HSA Modulates RA-FLS Inflammatory Response

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RA-FLSs were stimulated with the indicated concentration of AOPP-HSA (50, 100, or 200 µg/ml) for 48 hours or treated with 100 µg/ml AOPP-HSA for the indicated time (6, 12, 24, or 48 hours). Then, cell supernatants were collected and centrifuged at 12,000 g for 15 minutes. Supernatants were stored at -80°C until use. The levels of IL-6, TNFα (Boster, China), MMP-3, and MMP-13 (R&D Systems, USA) in the supernatant were quantified using enzyme-linked immunoabsorbent assay (ELISA) kits according to the manufacturer’s protocol. The optical density (OD) was measured at 450 nm with a spectrophotometric plate reader.
In a blocking test, RA-FLSs were pretreated with or without a neutralizing anti-RAGE antibody (10 µg/ml) for one hour, followed by treatment with AOPP-HSA (100 µg/ml) for 48 hours. The levels of IL-6, TNFα, MMP-3, and MMP-13 were detected. All experiments were performed in triplicate.

Lou A., Wang L., Lai W., Zhu D., Wu W., Wang Z., Cai Z, & Yang M. (2021). Advanced oxidation protein products induce inflammatory responses and invasive behaviour in fibroblast-like synoviocytes via the RAGE-NF-κB pathway. Bone & Joint Research, 10(4), 259-268.

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Enzyme-Linked Immunosorbent Assay for MMP-10 Antibodies

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Recombinant human MMP-10 (R&D Systems) was diluted (0.1 μg/ml) in carbonate coating buffer, added to Immulon 1B ELISA plates (Thermo Scientific), and incubated overnight at 4 °C. All remaining incubations were conducted on a platform shaker set at 200 revolutions per minute at room temperature. The plates were incubated with a 3% bovine serum albumin (BSA; Equitech-Bio, Inc.) in PBS–0.05% Tween 20 (PBST) blocking buffer. Plates were incubated with patient serum or joint fluid samples (1:200) or the positive control anti-MMP-10 MAb910 (1:200; R&D Systems). Following washes with PBST, horseradish peroxidase–conjugated goat anti-human IgG (Santa Cruz Biotechnology) or horseradish peroxidase–conjugated donkey anti-mouse IgG (Santa Cruz Biotechnology) was added, followed by TMB substrate (BD Biosciences). For interplate standardization, the positive control MAb910 was included on each plate. A positive antibody response was defined as >3 SD above the mean in 58 healthy subjects; this mean + 3 SD value characteristically corresponded to >0.51 OD₄₅₀.
To examine specificity of the antibody response to MMP-10, serum samples were also tested with MMP-3 (R&D Systems), using the same methods detailed for MMP-10. Seropositivity responses to the B. burgdorferi were analyzed by ELISA and Western blot as previously described [49 (link)–51 (link)].

Crowley J.T., Strle K., Drouin E.E., Pianta A., Arvikar S.L., Wang Q., Costello C.E, & Steere A.C. (2016). Matrix metalloproteinase-10 is a target of T and B cell responses that correlate with synovial pathology in patients with antibiotic-refractory Lyme arthritis. Journal of autoimmunity, 69, 24-37.

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Nobiletin Modulates Inflammatory Signaling

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Nobiletin was purchased from Cayman Chemical (Ann Arbor, MI, USA). Recombinant human IL-1β was obtained from Peprotech (Rocky Hill, NJ, USA). Antibodies against intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 were purchased from BioLegend (San Diago, CA, USA). Antibodies against phospho-p38 MAPK, phospho-extracellular signal-regulated kinase (ERK), phospho-c-Jun N-terminal kinase (JNK), phospho-IkappaB kinase (IKK)-α/β, phospho-NF-κB p65, phospho-Akt, p38 MAPK, ERK, JNK, IKK-α, NF-κB p65, Akt, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Cell Signaling Technology (Danvers, MA, USA). Enzyme-linked immunosorbent assay (ELISA) kits of IL-6, IL-8, CXCL10, CCL2, CCL20, MMP-1, MMP-3, and TIMP-1 were purchased from R&D Systems (Minneapolis, MN, USA).

Hosokawa Y., Hosokawa I., Ozaki K, & Matsuo T. (2021). Nobiletin Inhibits Inflammatory Reaction in Interleukin-1β-Stimulated Human Periodontal Ligament Cells. Pharmaceutics, 13(5), 667.

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ELISA Assay for MMP-1, MMP-3, and IL-6

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ELISA Kits for MMP-1, MMP-3, and IL-6 were purchased from R&D Systems, Inc. (Minneapolis, MN). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and penicillin–streptomycin were purchased from Gibco BRL (Grand Island, NY). Unless otherwise mentioned, solvents were purchased from Samchun Chemicals (Seoul, Korea), and inorganic salts from Sigma-Aldrich (St. Louis, MO).

Zhang M., Hwang E., Lin P., Gao W., Ngo H.T, & Yi T.H. (2018). Prunella vulgaris L. Exerts a Protective Effect Against Extrinsic Aging Through NF-κB, MAPKs, AP-1, and TGF-β/Smad Signaling Pathways in UVB-Aged Normal Human Dermal Fibroblasts. Rejuvenation Research, 21(4), 313-322.

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Cytokine and MMP Profiling in Culture

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Concentration of IL-17A, IL-17F, IL-22, IFNγ, GM-CSF, IL-6, IL-8 (all eBioscience), MMP1, and MMP3 (both R&D systems) were measured in culture supernatant using ELISA following manufacturer’s protocols.

Dankers W., den Braanker H., Paulissen S.M., van Hamburg J.P., Davelaar N., Colin E.M, & Lubberts E. (2021). The heterogeneous human memory CCR6+ T helper-17 populations differ in T-bet and cytokine expression but all activate synovial fibroblasts in an IFNγ-independent manner. Arthritis Research & Therapy, 23, 157.

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Serum Biomarker Profiling Protocol

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Serum samples were obtained preoperatively or before white light cystoscopy. Venous blood (10 ml) was collected and centrifuged at 900 rpm for 10 minutes. The serum was then separated, aliquoted in 2 ml fractions and stored at −80°C until further use. Protein serum levels were assessed by the multiplexed particle-based flow cytometric cytokine assay (Vignali, 2000). Kits for VEGF-A, VEGF-C and VEGF-D were purchased from Millipore (Zug, Switzerland), for MMP-2, MMP-3 and MMP-7 from R&D Systems (Oxon, UK) and for VEGF-R2 and VEGF-R3 from eBioscience (Vienna, Austria). The procedures closely followed the manufacturer’s instructions. The analysis was conducted using a conventional flow cytometer (Guava EasyCyte Plus, Millipore, Zug, Switzerland) [22 (link)].

Benoit T., Keller E.X., Wolfsgruber P., Hermanns T., Günthart M., Banzola I., Sulser T., Provenzano M, & Poyet C. (2015). High VEGF-D and Low MMP-2 Serum Levels Predict Nodal-Positive Disease in Invasive Bladder Cancer. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 21, 2266-2274.

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Quantifying Secretome Composition

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Supernatants from MenSCs/EECs co-cultures were enzyme-linked immunosorbent assay (ELISA)-tested using commercially available EGF, PDGF, FGF, MMP3, VEGF, and TGF-β1 ELISA kits (R&D systems, USA). The optical density of the wells was measured at 450 nm using spectrophotometric methods with microplate readers.

Zhao M., Chi F., Zhang T., Teng X, & Li K. (2021). Human menstrual blood-derived mesenchymal stem cells regulation of the EGF/Ras p21 pathway as a potential therapeutic target for thin endometrium. Annals of Translational Medicine, 9(18), 1476.

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Measuring Serum MMP-3 Levels

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Blood samples were taken to determine expression levels of matrix‐degrading MMP‐3 (1:20 serum dilution; R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocol.

Hayer S., Zeilinger M., Weiss V., Dumanic M., Seibt M., Niederreiter B., Shvets T., Pichler F., Wadsak W., Podesser B.K., Helbich T.H., Hacker M., Smolen J.S., Redlich K, & Mitterhauser M. (2019). Multimodal [18F]FDG PET/CT Is a Direct Readout for Inflammatory Bone Repair: A Longitudinal Study in TNFα Transgenic Mice. Journal of Bone and Mineral Research, 34(9), 1632-1645.

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ELISA Analysis of Inflammatory Mediators

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Concentrations of IL-6, MMP-1, MMP-3, MMP-13 and PGE₂ in medium samples were determined by enzyme-linked immunosorbent assay (ELISA) with commercial reagents (PGE₂: Cayman Chemical Co., Ann Arbor, MI, USA; human IL-6: eBioscience Inc. San Diego, CA, USA; MMP-1, MMP-3, MMP-13 and mouse IL-6: R&D Systems Europe Ltd).

Nummenmaa E., Hämäläinen M., Moilanen L.J., Paukkeri E.L., Nieminen R.M., Moilanen T., Vuolteenaho K, & Moilanen E. (2016). Transient receptor potential ankyrin 1 (TRPA1) is functionally expressed in primary human osteoarthritic chondrocytes. Arthritis Research & Therapy, 18, 185.

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Measuring Inflammatory Markers in RAFLS

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Ninety-six multi-well plate was used for seeding 10,000 RAFLS for performing ELISA. For each condition, 3 replicate wells were established. The inhibitors were added under high glucose (25 mM) or glucose-free conditions 1 h prior to TNF alpha stimulation (5 ng/ml) and incubated for 24 h at 37 °C. The next day, the supernatant was aspired and IL-6, MMP3, and CCL2 (R&D systems) were measured as per the manufacturer’s instructions.

Ahmed S., Mahony C.B., Torres A., Murillo-Saich J., Kemble S., Cedeno M., John P., Bhatti A., Croft A.P, & Guma M. (2023). Dual inhibition of glycolysis and glutaminolysis for synergistic therapy of rheumatoid arthritis. Arthritis Research & Therapy, 25, 176.

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