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Hgastrin 1

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HGastrin I is a lab equipment product developed by Bio-Techne. It is used to detect and measure the presence of gastrin, a peptide hormone produced in the stomach and upper small intestine. The core function of HGastrin I is to provide a reliable and accurate method for researchers and clinicians to quantify gastrin levels in biological samples.

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Lab products found in correlation

Glutamax, by Thermo Fisher Scientific (4 mentions) B27 supplement, by Thermo Fisher Scientific (4 mentions) L wrn, by American Type Culture Collection (2 mentions) Human epidermal growth factor (hegf), by Thermo Fisher Scientific (2 mentions) Hfgf10, by Thermo Fisher Scientific (2 mentions) Hepes, by Thermo Fisher Scientific (2 mentions) Advanced dmem f12, by Thermo Fisher Scientific (2 mentions) A83 01, by Bio-Techne (2 mentions) Y 27632, by Selleck Chemicals (2 mentions) N acetylcysteine, by Merck Group (2 mentions) Nicotinamide, by Merck Group (2 mentions) Growth factor reduced matrigel, by Corning (2 mentions)

2 protocols using hgastrin 1

1

Culturing Patient-Derived Pancreatic Cancer Organoids

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The human pancreatic cancer organoids were provided by Dr. David Tuveson (Cold Spring Harbor Laboratory). The patient derived PDAC organoids were cultured at 37°C in 5% CO2. Cells were initially seeded in growth factor reduced Matrigel (Corning) domes and after reseeding were fed with complete human feeding medium: advanced DMEM/F12 (Thermo Fisher Scientific) based WRN conditioned medium (L-WRN (ATCC CRL-3276)), 1x B27 supplement (Thermo Fisher Scientific), 10 mM HEPES (Thermo Fisher Scientific), 0.01 μM GlutaMAX (Thermo Fisher Scientific), 10 mM nicotinamide (Sigma-Aldrich), 1.25 mM N-acetylcysteine (Sigma-Aldrich), 50 ng/mL hEGF (Peprotech), 100 ng/mL hFGF10 (Peprotech), 0.01 μM hGastrin I (TOCRIS), 500 nM A83-01 (TOCRIS), and 10.5 μM Y27632 (Selleckchem) (Boj et al., 2015 (link)). Organoids were tested negative for mycoplasma.
Waters A.M., Khatib T.O., Papke B., Goodwin C.M., Hobbs G.A., Diehl J.N., Yang R., Edwards A.C., Walsh K.H., Sulahian R., McFarland J.M., Kapner K.S., Gilbert T.S., Stalnecker C.A., Javaid S., Barkovskaya A., Grover K.R., Hibshman P.S., Blake D.R., Schaefer A., Nowak K.M., Klomp J.E., Hayes T.K., Kassner M., Tang N., Tanaseichuk O., Chen K., Zhou Y., Kalkat M., Herring L.E., Graves L.M., Penn L.Z., Yin H.H., Aguirre A.J., Hahn W.C., Cox A.D, & Der C.J. (2021). Targeting p130Cas- and microtubule-dependent MYC regulation sensitizes pancreatic cancer to ERK MAPK inhibition. Cell reports, 35(13), 109291.
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2

Culturing Patient-Derived Pancreatic Cancer Organoids

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The human pancreatic cancer organoids were provided by Dr. David Tuveson (Cold Spring Harbor Laboratory). The patient derived PDAC organoids were cultured at 37°C in 5% CO2. Cells were initially seeded in growth factor reduced Matrigel (Corning) domes and after reseeding were fed with complete human feeding medium: advanced DMEM/F12 (Thermo Fisher Scientific) based WRN conditioned medium (L-WRN (ATCC CRL-3276)), 1x B27 supplement (Thermo Fisher Scientific), 10 mM HEPES (Thermo Fisher Scientific), 0.01 μM GlutaMAX (Thermo Fisher Scientific), 10 mM nicotinamide (Sigma-Aldrich), 1.25 mM N-acetylcysteine (Sigma-Aldrich), 50 ng/mL hEGF (Peprotech), 100 ng/mL hFGF10 (Peprotech), 0.01 μM hGastrin I (TOCRIS), 500 nM A83-01 (TOCRIS), and 10.5 μM Y27632 (Selleckchem) (Boj et al., 2015 (link)). Organoids were tested negative for mycoplasma.
Waters A.M., Khatib T.O., Papke B., Goodwin C.M., Hobbs G.A., Diehl J.N., Yang R., Edwards A.C., Walsh K.H., Sulahian R., McFarland J.M., Kapner K.S., Gilbert T.S., Stalnecker C.A., Javaid S., Barkovskaya A., Grover K.R., Hibshman P.S., Blake D.R., Schaefer A., Nowak K.M., Klomp J.E., Hayes T.K., Kassner M., Tang N., Tanaseichuk O., Chen K., Zhou Y., Kalkat M., Herring L.E., Graves L.M., Penn L.Z., Yin H.H., Aguirre A.J., Hahn W.C., Cox A.D, & Der C.J. (2021). Targeting p130Cas- and microtubule-dependent MYC regulation sensitizes pancreatic cancer to ERK MAPK inhibition. Cell reports, 35(13), 109291.
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