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Rabbit anti β catenin

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Rabbit anti-β-catenin is a primary antibody that specifically recognizes the β-catenin protein. β-catenin is a key component of the Wnt signaling pathway and is involved in cell-cell adhesion and gene transcription regulation. This antibody can be used for various applications, such as western blotting, immunohistochemistry, and immunofluorescence, to detect and analyze the expression and localization of β-catenin in biological samples.

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Lab products found in correlation

Mouse anti flag, by Merck Group (3 mentions) Rabbit anti e cadherin, by Cell Signaling Technology (2 mentions) Rabbit anti gapdh, by Merck Group (2 mentions) Mouse anti β tubulin, by Merck Group (2 mentions) Mouse anti lamp1, by BD (2 mentions) Rabbit anti plk4, by Cell Signaling Technology (2 mentions) Rat anti zo 1, by Santa Cruz Biotechnology (2 mentions) Rabbit anti prp6, by Santa Cruz Biotechnology (2 mentions) Mouse anti prpf8, by Santa Cruz Biotechnology (2 mentions) Rabbit anti prpf8, by Santa Cruz Biotechnology (2 mentions) Goat anti prpf31, by Abnova (2 mentions) Pibf1 cep90, by Novus Biologicals (2 mentions) Rabbit anti gpr20 polyclonal, by Thermo Fisher Scientific (2 mentions) Rabbit anti sreb3, by Novus Biologicals (2 mentions) Rabbit anti mas1, by Novus Biologicals (2 mentions) Rabbit anti htr1b, by Novus Biologicals (2 mentions) Rabbit anti p2ry14, by Novus Biologicals (2 mentions) Alx 804 885 c100, by Merck Group (2 mentions) Lsm 800, by Zeiss (1 mentions) Mouse anti e cadherin, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

β-catenin (387 citations) Anti-β-catenin (221 citations) Rabbit anti-β-catenin monoclonal (2 citations)

7 protocols using rabbit anti β catenin

1

Immunofluorescence Characterization of Stem Cells

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Immunofluorescence was performed as described.33 (link) The following primary antibodies and dilutions were used: rat anti-CD31 1 : 1000 (BD, Franklin Lakes, NJ, USA), mouse anti-Tuj-1 1 : 1000 (Covance, Princeton, NJ, USA), mouse anti-AFP 1 : 1000 (Inmunostep, Salamanca, Spain), rabbit anti-Nanog 1:1000 (Chemicon, Billerica, MA, USA), mouse anti-SSEA-1 (MC-480) 1 : 100 (Pierce, Waltham, MA, USA), rabbit anti-E-cadherin 1 : 60 (Cell Signaling, MA, USA), mouse anti-E-cadherin 1:200 (Cell Signaling, Danvers, MA, USA), rabbit anti-β-catenin 1 : 200 (BD). Secondary antibodies were: Alexa 647 goat anti-IgG rabbit (Molecular Probes, Eugene, OR, USA), Alexa 488 goat anti-IgG mouse (Molecular Probes), Alexa 568 donkey anti-IgG rat (Molecular Probes), Cy3 donkey anti-IgG rabbit (Jackson Immunoresearch, West Grove, PA, USA) and FITC donkey anti-IgG mouse (Jackson Immunoresearch). Images were obtained with NIKON EclipseTE2000 and ZEISS LSM 800 confocal microscope.
Martin-Lopez M., Maeso-Alonso L., Fuertes-Alvarez S., Balboa D., Rodríguez-Cortez V., Weltner J., Diez-Prieto I., Davis A., Wu Y., Otonkoski T., Flores E.R., Menéndez P., Marques M.M, & Marin M.C. (2017). p73 is required for appropriate BMP-induced mesenchymal-to-epithelial transition during somatic cell reprogramming. Cell Death & Disease, 8(9), e3034-.
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2

Antibody Panel for Cell Signaling Analysis

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The following primary antibodies (at the dilution indicated) were used for either immunofluorescence or Western blotting: mouse anti-p21 (1:100, cod. B1313; Santa Cruz, Biotechnology, Dallas, TX, USA); rabbit anti-Ki67 (1:100, cod. HPA001164; Sigma-Aldrich, St. Louis, MO, USA); rabbit anti-phospho-ERK1/2 (Thr202/Tyr204, Thr185/Tyr187) (1:500, cod. 05–797R; Millipore, Burlington, MA, USA); mouse anti-ERK1/2 (1:500, cod. 05–1152; Millipore, Burlington, MA, USA); rabbit anti-phospho-S6 (Ser235/236) (1:500, cod. 4856; Cell Signaling, Danvers, MA, USA); rabbit anti-S6 (1:500, cod. 2217; Cell Signaling, Danvers, MA, USA); mouse anti-N-Cadherin (1:50, cod. 610920; BD Biosciences, Franklin Lakes, NJ); mouse anti-E-Cadherin (1:50, cod. 610404; BD Biosciences, Franklin Lakes, NJ); rabbit anti-LC3 (1:1000, cod. L7543; Sigma-Aldrich, St. Louis, MO, USA); mouse anti-LAMP1 (1:1000, cod. 555798; BD, Biosciences, Franklin Lakes, NJ); rabbit anti- β-Catenin (1:500, cod. PA5-77934; Invitrogen, Paisley, UK); mouse anti-β-Actin (1:2000, cod. A5441; Sigma-Aldrich, St. Louis, MO, USA); mouse anti-β-Tubulin (1:1000, cod. T5326; Sigma-Aldrich, St. Louis, MO, USA); rabbit anti-GAPDH (1:1000, cod. G9545; Sigma-Aldrich, St. Louis, MO, USA).
Vallino L., Garavaglia B., Visciglia A., Amoruso A., Pane M., Ferraresi A, & Isidoro C. (2023). Cell-free Lactiplantibacillus plantarum OC01 supernatant suppresses IL-6-induced proliferation and invasion of human colorectal cancer cells: Effect on β-Catenin degradation and induction of autophagy. Journal of Traditional and Complementary Medicine, 13(2), 193-206.
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3

Immunofluorescent Staining Protocol for Various Antibodies

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The following antibodies were used with the indicated titres for immunofluorescent staining: rabbit anti-PRP6 (Santa Cruz sc-48786) 1:100, mouse anti-PRPF8 (Santa Cruz sc-55534) 1:100, rabbit anti-PRPF8 (Santa Cruz sc-030207) 1:100, goat anti-PRPF31 (Abnova PAB7154) 1:100, PIBF1/CEP90 (Novus NBP2-19823) 1:100, rabbit anti-PLK4 (Cell Signalling Technologies 3258) 1:100, rabbit anti-GPR20 polyclonal (Thermo Scientific, PA3-068) 1:100, rabbit anti-SREB3 (Novus Biologicals NBP1-71064) 1:100, rabbit anti-MAS1 (Novus Biologicals NBP1-78444) 1:100, rabbit anti-HTR1B (Novus Biologicals NBP1-01013) 1:100, rabbit anti-P2RY14 (Novus NBP1-70970) 1:100, rabbit anti-CRHR2 (Novus NBP1-00767) 1:100, rabbit anti-OPRL1 (Abcam ab66219) 1:50, rabbit anti-C21orf2 (GeneTex [N1C2], GTX119046) 1:100, mouse anti-acetylated alpha tubulin (Sigma Aldrich clone 6-11 B1 T6793) 1:1000, rat anti-ZO1 (1:400, Santa Cruz), rabbit anti-β-catenin (1:400, BD Bioscience), rabbit anti-gamma tubulin (AbCam ab11317) 1:1000, rabbit anti-ARL13B (Proteintech 17711-1-AP) 1:1000, mouse anti-GT335 monoclonal (Enzo Life Sciences ALX-804-885-C100) 1:1000 and mouse anti-FLAG (Sigma F3165) 1:1000. The mouse monoclonal antibody against centrin-3 has been described previously35 (link). Secondary antibodies were Alexa-Fluor-conjugated goat anti-mouse IgG and goat anti-rabbit IgG (Life Technologies Ltd.)
Wheway G., Schmidts M., Mans D.A., Szymanska K., Nguyen T.M., Racher H., Phelps I.G., Toedt G., Kennedy J., Wunderlich K.A., Sorusch N., Abdelhamed Z.A., Natarajan S., Herridge W., van Reeuwijk J., Horn N., Boldt K., Parry D.A., Letteboer S.J., Roosing S., Adams M., Bell S.M., Bond J., Higgins J., Morrison E.E., Tomlinson D.C., Slaats G.G., van Dam T.J., Huang L., Kessler K., Giessl A., Logan C.V., Boyle E.A., Shendure J., Anazi S., Aldahmesh M., Al Hazzaa S., Hegele R.A., Ober C., Frosk P., Mhanni A.A., Chodirker B.N., Chudley A.E., Lamont R., Bernier F.P., Beaulieu C.L., Gordon P., Pon R.T., Donahue C., Barkovich A.J., Wolf L., Toomes C., Thiel C.T., Boycott K.M., McKibbin M., Inglehearn C.F., Stewart F., Omran H., Huynen M.A., Sergouniotis P.I., Alkuraya F.S., Parboosingh J.S., Innes A.M., Willoughby C.E., Giles R.H., Webster A.R., Ueffing M., Blacque O., Gleeson J.G., Wolfrum U., Beales P.L., Gibson T., Doherty D., Mitchison H.M., Roepman R, & Johnson C.A. (2015). An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes. Nature cell biology, 17(8), 1074-1087.
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4

Immunofluorescent Staining Protocol for Various Antibodies

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The following antibodies were used with the indicated titres for immunofluorescent staining: rabbit anti-PRP6 (Santa Cruz sc-48786) 1:100, mouse anti-PRPF8 (Santa Cruz sc-55534) 1:100, rabbit anti-PRPF8 (Santa Cruz sc-030207) 1:100, goat anti-PRPF31 (Abnova PAB7154) 1:100, PIBF1/CEP90 (Novus NBP2-19823) 1:100, rabbit anti-PLK4 (Cell Signalling Technologies 3258) 1:100, rabbit anti-GPR20 polyclonal (Thermo Scientific, PA3-068) 1:100, rabbit anti-SREB3 (Novus Biologicals NBP1-71064) 1:100, rabbit anti-MAS1 (Novus Biologicals NBP1-78444) 1:100, rabbit anti-HTR1B (Novus Biologicals NBP1-01013) 1:100, rabbit anti-P2RY14 (Novus NBP1-70970) 1:100, rabbit anti-CRHR2 (Novus NBP1-00767) 1:100, rabbit anti-OPRL1 (Abcam ab66219) 1:50, rabbit anti-C21orf2 (GeneTex [N1C2], GTX119046) 1:100, mouse anti-acetylated alpha tubulin (Sigma Aldrich clone 6-11 B1 T6793) 1:1000, rat anti-ZO1 (1:400, Santa Cruz), rabbit anti-β-catenin (1:400, BD Bioscience), rabbit anti-gamma tubulin (AbCam ab11317) 1:1000, rabbit anti-ARL13B (Proteintech 17711-1-AP) 1:1000, mouse anti-GT335 monoclonal (Enzo Life Sciences ALX-804-885-C100) 1:1000 and mouse anti-FLAG (Sigma F3165) 1:1000. The mouse monoclonal antibody against centrin-3 has been described previously35 (link). Secondary antibodies were Alexa-Fluor-conjugated goat anti-mouse IgG and goat anti-rabbit IgG (Life Technologies Ltd.)
Wheway G., Schmidts M., Mans D.A., Szymanska K., Nguyen T.M., Racher H., Phelps I.G., Toedt G., Kennedy J., Wunderlich K.A., Sorusch N., Abdelhamed Z.A., Natarajan S., Herridge W., van Reeuwijk J., Horn N., Boldt K., Parry D.A., Letteboer S.J., Roosing S., Adams M., Bell S.M., Bond J., Higgins J., Morrison E.E., Tomlinson D.C., Slaats G.G., van Dam T.J., Huang L., Kessler K., Giessl A., Logan C.V., Boyle E.A., Shendure J., Anazi S., Aldahmesh M., Al Hazzaa S., Hegele R.A., Ober C., Frosk P., Mhanni A.A., Chodirker B.N., Chudley A.E., Lamont R., Bernier F.P., Beaulieu C.L., Gordon P., Pon R.T., Donahue C., Barkovich A.J., Wolf L., Toomes C., Thiel C.T., Boycott K.M., McKibbin M., Inglehearn C.F., Stewart F., Omran H., Huynen M.A., Sergouniotis P.I., Alkuraya F.S., Parboosingh J.S., Innes A.M., Willoughby C.E., Giles R.H., Webster A.R., Ueffing M., Blacque O., Gleeson J.G., Wolfrum U., Beales P.L., Gibson T., Doherty D., Mitchison H.M., Roepman R, & Johnson C.A. (2015). An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes. Nature cell biology, 17(8), 1074-1087.
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5

Antibody selection and usage in cellular analysis

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The following primary antibodies (at the dilution indicated) were used for either immunofluorescence or Western blotting: rabbit anti-Ki67 (1:100, cod. HPA001164; Sigma-Aldrich, St. Louis, MO, USA); mouse anti-p21 (1:100, cod. B1313; Santa Cruz Biotechnology, Dallas, TX, USA); mouse anti-Histone H3 (1:500, cod. 61475; Active motif, Carlsbad, San Diego, CA, USA); mouse anti-LC3 (1:100, cod. NB600-1384; Novus Biologicals, Milano, Italy); rabbit anti-LC3 (1:1000, cod. L7543; Sigma-Aldrich, St. Louis, MO, USA); mouse anti-LAMP1 (1:1000, cod. 555798; BD, Biosciences, Franklin Lakes, NJ, USA); rabbit anti-β-Catenin (1:500, cod. PA5-77934; Invitrogen, Paisley, UK); rabbit-anti ATG7 (1:500, cod. AB10511; Millipore, Burlington, MA, USA); mouse-anti ATG7 (1:500, cod. SAB4200304; Sigma-Aldrich, St. Louis, MO, USA); mouse anti-β-tubulin (1:1000, cod. T5326; Sigma-Aldrich, St. Louis, MO, USA); rabbit anti-GAPDH (1:1000, cod. G9545; Sigma-Aldrich, St. Louis, MO, USA); mouse anti-β-actin (1:2000, cod. A5441; Sigma-Aldrich, St. Louis, MO, USA).
Garavaglia B., Vallino L., Ferraresi A., Esposito A., Salwa A., Vidoni C., Gentilli S, & Isidoro C. (2022). Butyrate Inhibits Colorectal Cancer Cell Proliferation through Autophagy Degradation of β-Catenin Regardless of APC and β-Catenin Mutational Status. Biomedicines, 10(5), 1131.
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6

Antibody Characterization Protocol

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The anti-NterSOX9/MS9 antibody was obtained using a standard rabbit immunization procedure with the synthetic peptide DTENTRPQENTFPKGC. The antibody was purified from the rabbit serum by affinity with the synthetic peptide. The anti-Cter-SOX9 antibody was previously described [5 (link)]. Anti-c-myc (9E10) and anti-α-tubulin were from home-made hybridomas. The mouse anti-flag was from Sigma-Aldrich, (Lyon, France) and the rabbit anti-β-catenin was from BD Transduction Lab (Le-Pont-de-Claix, France).
Prévostel C., Rammah-Bouazza C., Trauchessec H., Canterel-Thouennon L., Busson M., Ychou M, & Blache P. (2016). SOX9 is an atypical intestinal tumor suppressor controlling the oncogenic Wnt/ß-catenin signaling. Oncotarget, 7(50), 82228-82243.
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7

Immunofluorescence Analysis of Intestinal Epithelial Markers

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For immunofluorescence staining, duodenum, jejunum, ileum and colon tissues from WT and EpCAM−/− mice were fixed overnight with 4% para-formaldehyde in PBS at 4°C before being dehydrated and embedded in optimal cutting temperature compound (OCT) (Sakura Finetek). Then, 7-µm frozen sections were boiled in 10 mM citric acid (Merck) at pH 6.0 for 5 min. After exposure to goat serum blocking buffer (ZSGB-BIO, ZLI-9056) at room temperature for 1 h, the sections were incubated overnight at 4°C with primary antibodies and then with secondary antibodies at room temperature for 1 h. Primary antibodies were used as follows: Rabbit anti-E-cadherin (Cell Signaling Technology, Inc.; cat. no. 14472, 1:200), rabbit anti-p120-catenin (Santa Cruz Biotechnology, Inc.; cat. no. 15D2, 1:200), and rabbit anti-β-catenin (BD Biosciences; cat. no. 610154, 1:200). Immunofluorescence analysis was performed with Alexa Fluor 488-labeled donkey anti-rabbit IgG (H+L) secondary antibodies (Thermo Fisher Scientific, Inc.; cat. no. A21206, 1:1,000), and DAPI (Sigma-Aldrich; Merck KGaA; cat. no. D9564, 1:10,000) was used to stain the nuclei of tissues. Immunofluorescence images were observed using a PerkinElmer Automated Quantitative Pathology System.
Chen G., Yang Y., Liu W., Huang L., Yang L., Lei Y., Wu H., Lei Z, & Guo J. (2020). EpCAM is essential for maintenance of the small intestinal epithelium architecture via regulation of the expression and localization of proteins that compose adherens junctions. International Journal of Molecular Medicine, 47(2), 621-632.
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