Penicillin streptomycin sulfate

Manufactured by Thermo Fisher Scientific

Sourced in United States

Penicillin-streptomycin sulfate is a laboratory reagent that is commonly used as an antibiotic supplement in cell culture media. It contains a combination of the antibiotics penicillin and streptomycin, which inhibit the growth of a wide range of bacteria.

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Penicillin/streptomycin (33948 citations) Streptomycin (24872 citations) Penicillin (24825 citations) Streptomycin sulfate (466 citations) Penicillin G-streptomycin sulfate (14 citations) Penicillin and streptomycin sulfate (6 citations) Penicillin G/streptomycin sulfate solution (4 citations) Penicillin G and streptomycin sulfate (2 citations)

39 protocols using penicillin streptomycin sulfate

Gastric Cancer Stem Cell Isolation

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The human gastric SNU-5 and BGC-823 cell lines were purchased from the Chinese Academy of Sciences Cell Bank. SNU-5 gastric cancer cells were maintained in RPMI-1640 medium (Thermo Fisher Scientific, USA) supplemented with 10% FBS (Thermo Fisher Scientific), 1% L-glutamine (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin sulfate (Thermo Fisher Scientific). BGC-823 gastric cancer cells were maintained in DH medium (Thermo Fisher Scientific) supplemented with 10% FBS (Thermo Fisher Scientific), 1% L-glutamine (Gibco) and 1% penicillin-streptomycin sulfate (Thermo Fisher Scientific).
The serum-free medium (SFM) was composed of DMEM/F12, 20 µl/ml B27 supplement (Life Technologies), 20 ng/ml basic fibroblast growth factor (bFGF, Gibco), 10 ng/ml EGF (Gibco) and LIF (Gibco). GCSCs were isolated from SNU-5 or BGC-823 cell line by using SFM. These cells can form sphere-like cell aggregates in less than 7 days. All cultures were maintained in a 37°C incubator supplemented with 5% CO₂.

Pan Y., Shu X., Sun L., Yu L., Sun L., Yang Z, & Ran Y. (2017). miR-196a-5p modulates gastric cancer stem cell characteristics by targeting Smad4. International Journal of Oncology, 50(6), 1965-1976.

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Cell Culture Conditions for Esophageal and Gastric Cancer Lines

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Human esophageal cancer cells lines OE19, ESO26, OACP4C, and SKGT4 cells were grown in RPMI-1640 medium (Corning, #45000-396). Flo-1 cells were cultured in Dulbecco’s modified Eagle medium (DMEM) (Corning, #45000-304). Both media were supplemented with 10% FBS (Omega Scientific, #FB-02) and 1% penicillin-streptomycin sulfate (Gibco, #10378016). Murine gastric cancer cells, YTN 5 and YTN16 were kindly provided by Dr. Sachiyo Nomura at The University of Tokyo. YTN5 and YTN16 cells were cultured in DMEM (Corning, #45000-304), with 10% heat-inactivated fetal bovine serum (Omega Scientific, #FB-02) and 1% penicillin-streptomycin sulfate (Gibco, #10378016), 1X GlutaMAX (Gibco, #35050-061) and MITO+ serum extender (Corning, #355006). All cultures were maintained in a 37 °C incubator supplemented with 5% CO₂. All the cell lines were tested for mycoplasma and verified by us using short tandem repeat analysis.

Ziman B., Yang Q., Zheng Y., Sheth M., Nam C., Zhao H., Zhang L., Hu B., Bhowmick N.A., Sinha U.K, & Lin D.C. (2024). Epigenomic analyses identify FOXM1 as a key regulator of anti-tumor immune response in esophageal adenocarcinoma. Cell Death & Disease, 15(2), 152.

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Cell Culture of Neural Progenitors and HEK293T

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C17.2 mouse neural progenitor cells were cultured in DMEM (Gibco, 41965-039) supplemented with 10% Fetal Bovine Serum (FBS, Bodinco, 5010), 5% Horse Serum (Invitrogen, 26050-088) and 1% Penicillin-Streptomycin sulfate (Gibco, 15140-122) at 37°C under 5 % CO₂. Human embryonic kidney cells stably transfected with SV40 large T antigen (HEK293T) were cultured in DMEM (Gibco 41965-039) supplemented with 10% Fetal Bovine Serum (Bodinco, 5010) and 1% Penicillin-Streptomycin sulfate (Gibco, 15140-122) at 37°C under 5 % CO₂.

Joshi B.S., Youssef S.A., Bron R., de Bruin A., Kampinga H.H, & Zuhorn I.S. (2021). DNAJB6b-enriched small extracellular vesicles decrease polyglutamine aggregation in in vitro and in vivo models of Huntington disease. iScience, 24(11), 103282.

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Cultivation of Human OSCC Cell Lines

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The human OSCC cell lines Ca9-22 (cat#: JCRB0625) and HSC-2 (cat#: JCRB0622) were obtained from the Japanese Collection of Research Bioresources (JCRB) cell bank (Osaka, Japan). Ca9-22 cells were isolated from a primary gingival carcinoma [47 (link)] and HSC-2 cells were isolated from cervical lymph node metastatic SCC derived from the floor of the oral cavity [48 (link)]. These cells are considered to be highly differentiated [47 (link),48 (link)]. Cells were grown in RPMI-1640 containing 10% fetal bovine serum (FBS; Bio West, Miami, FL, USA) and 1% penicillin/streptomycin sulfate (Thermo Fisher Scientific, Waltham, MA, USA), hereafter designated as complete medium. For passaging, cells were washed with phosphate-buffered saline (PBS; Thermo Fisher Scientific). Single-cell suspensions were obtained using 0.25% trypsin/0.01% EDTA (Thermo Fisher Scientific) and adjusted to the desired cell numbers for experiments.

Ushio R., Hiroi M., Matsumoto A., Mori K., Yamamoto N, & Ohmori Y. (2022). Enhanced Cytotoxic Effects in Human Oral Squamous Cell Carcinoma Cells Treated with Combined Methyltransferase Inhibitors and Histone Deacetylase Inhibitors. Biomedicines, 10(4), 763.

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Gastric Cancer Spheroid Formation

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Gastric cancer cells were plated in each well of ultra-low-attachment 24-well plates (Corning Life Sciences, Corning, NY, USA) at low density (500 cells per each well) with 0.8% methyl cellulose (Sigma, USA) supplemented with 20 µl/ml B27 supplement (Life Technologies), 20 ng/ml basic fibroblast growth factor (bFGF, Gibco), 10 ng/ml EGF (Gibco), LIF (Gibco), 1% L-glutamine (Gibco) and 1% penicillin-streptomycin sulfate (Thermo Fisher Scientific). Every 3 days, each well was examined using light microscopy.

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Maintenance of Gastric Cancer Cell Lines

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MKN45 (KCLB 80103), AGS (ATCC CRL‐1739) and KATO III (KCLB 30103) gastric cancer cells were maintained in RPMI‐1640 medium (Thermo Scientific, Rockford, IL, USA) supplemented with 10% FBS (Thermo Scientific) and 1% penicillin‐streptomycin sulfate (Thermo Scientific). All cultures were maintained in a 37°C incubator supplemented with 5% CO₂.

Lee S.D., Yu D., Lee D.Y., Shin H., Jo J, & Lee Y.C. (2018). Upregulated microRNA‐193a‐3p is responsible for cisplatin resistance in CD44(+) gastric cancer cells. Cancer Science, 110(2), 662-673.

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Immortalized Stromal Cell Culture Protocol

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Stromal fibroblast cell lines HS5-GFP and HS27a-GFP were generously provided by the Torok-Storb laboratory [51 (link), 52 (link)]. These immortalized human marrow stromal lines were cultured in RPMI 1640 medium (Thermo Fisher Scientific) supplemented with l-glutamine (0.4 mg/mL, SAFC Biosciences), sodium pyruvate (1 mM/L), penicillin-streptomycin sulfate (100 μg/mL, Thermo Fisher Scientific), and 10% fetal bovine serum (FBS; Thermo Fisher Scientific). Stromal fibroblasts were cultured to 70% confluence in T-75 flasks and trypsinized prior to embedding in vessels. HS27a conditioned medium was removed after 5 days of culture and centrifuged prior to use in vessels for conditioned media experiments. Marrow MSCs were purchased from Lonza. MSCs were cultured in MSCGM (Lonza) in T-75 flasks and trypsinized prior to use.

Kotha S.S., Hayes B.J., Phong K.T., Redd M.A., Bomsztyk K., Ramakrishnan A., Torok-Storb B, & Zheng Y. (2018). Engineering a multicellular vascular niche to model hematopoietic cell trafficking. Stem Cell Research & Therapy, 9, 77.

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Culturing MG-63 and Saos-2 Osteosarcoma Cells

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MG-63 and Saos-2 cells were provided by the China Center for Type Culture Collection as in our previous study (Shu et al., 2020 (link)). MG-63 and Saos-2 cells were cultured in RPMI‑1640 and McCoy’s 5A medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), respectively, supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 1% L‑glutamine, and 1% penicillin‑streptomycin sulfate (Thermo Fisher Scientific, Inc., Waltham, MA, USA) at 37 °C in a 5% CO₂ humidity-controlled incubator (Chen et al., 2019 (link); Zhou et al., 2016 (link)).

Shu X., Liu W., Liu H., Qi H., Wu C, & Ran Y.L. (2021). Analysis of microRNA expression in CD133 positive cancer stem‑like cells of human osteosarcoma cell line MG-63. PeerJ, 9, e12115.

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3D Tumor Spheroid Formation Assay

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MG‑63 and Saos‑2 cells (500 cells/well) were plated in Ultra‑Low Attachment 24‑well plates (Corning, Inc., Corning, NY, USA) with 0.8% methyl cellulose (Sigma‑Aldrich, St. Louis, MO, USA; Merck KGaA, Darmstadt, Germany) supplemented with 20 μl/ml B27, 20 ng/ml bFGF, 10 ng/ml epidermal growth factor, 1% L‑glutamine and 1% penicillin‑streptomycin sulfate (all were obtained from Thermo Fisher Scientific, Inc., Waltham, MA, USA). Every 3 days, each well was examined under a light microscope (IX71; Olympus Corporation, Tokyo, Japan).

Shu X., Liu W., Liu H., Qi H., Wu C, & Ran Y.L. (2021). Analysis of microRNA expression in CD133 positive cancer stem‑like cells of human osteosarcoma cell line MG-63. PeerJ, 9, e12115.

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Cultivation of Human Esophageal Cancer Cell Lines

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Human esophageal cancer cell lines were kindly provided by the Stephen Meltzer’s laboratory from Johns Hopkins University. Flo-1, SKGT4, JH-EsoAd1 and OE33 were cultured in Dulbecco’s modified Eagle medium (DMEM, Gibco, USA) and TE7, KYSE30, KYSE140, KYSE150, OE19, ESO26, OACp4C and OACM5.1 were grown in RPMI-1640 medium (Gibco, USA). Both media were supplemented with 10% FBS (Omega Scientific, Tarzna, USA) and 1% penicillin-streptomycin sulfate (Thermo Scientific, USA). All cultures were maintained in a 37 °C incubator supplemented with 5% CO₂. All of the cell lines were tested for mycoplasma and verified by us using short tandem repeat analysis.

Ma S., Zhou B., Yang Q., Pan Y., Yang W., Freedland S.J., Ding L.W., Freeman M.R., Breunig J.J., Bhowmick N.A., Pan J., Phillip Koeffler H, & Lin D.C. (2021). A transcriptional regulatory loop of master regulator transcription factors, PPARG, and fatty acid synthesis promotes esophageal adenocarcinoma. Cancer research, 81(5), 1216-1229.

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