Total yap

For immunoblotting, primary antibodies against S6K1 (Thr³⁸⁹, #9234; total #2708), mTOR (Ser²⁴⁴⁸, #2971; total, #2983), 4E‐BP1 (Thr^37/46, #2855; total, #9644), eEF2 (Thr⁵⁶, #2331; total, #2332), JNK (Thr¹⁸³/Tyr¹⁸⁵, #4668), SMAD2 (Ser^245/250/255, #3104; total, #5339), TAZ (Ser⁸⁹, #59971), ACC (Ser⁷⁹, #3661; total, #3676), AMPK (Thr¹⁷², #4188; total, #2532), ULK1 (Ser³¹⁷, #12753; total, #8054), LC3b (#2775), GABARAP (#13733), BNIP3 (#44060) TSC2 (Ser¹³⁸⁷, #5584; total, #3635) PRAS40 (Thr²⁴⁶, #13175; total, #2691) and p38 (Thr¹⁸⁰/Thr¹⁸², #4511; total, #8690), were purchased from CST (Beverly, MA, USA). Primary antibody for total YAP (sc‐101199), total TAZ (sc‐293183), total JNK (sc‐7345), MuRF‐1 (#sc‐398608), MAFbx (#sc‐16806) and UBR5 (#sc‐515494) were purchased from Santa Cruz Biotechnology (Heidelberg, Germany). Primary antibody for YAP (Ser¹²⁷, #PA5‐17481) was purchased from Thermo Scientific. Primary antibody for REDD1 (#63059) was purchased from Abcam (Cambridge, UK). Primary antibody for HIF‐1α (#NB100‐134) was purchased from Novus Biologicals (Abingdon, UK).
All primary antibodies were diluted 1:1000 except for the total eEF2 and total 4E‐BP1, which were diluted 1:2000, and total JNK, total YAP, total TAZ, UBR5, MuRF‐1 and MAFbx which were diluted 1:500. Secondary anti‐rabbit (#7074; 1:10 000) and secondary anti‐mouse (#7076; 1:10 000) were purchased from CST.