Fam labeled sirna fam sirna

CAIX-targeting siRNA (CAIX-siRNA), negative control siRNA (NC-siRNA), and fluorescent carboxyfluorescein (FAM)-labeled siRNA (FAM-siRNA) were purchased from GenePharma Co, Ltd (Suzhou, Jiangsu, People’s Republic of China). The negative control for CAIX-siRNA was obtained with oligonucleotides: forward 5′–UUCUCCGAACGUGUCACGUTT–3 and reverse 5′–ACGUGACACGUUCGGAGAATT–3′. The sequences of CAIX-siRNA used were as follows: CAIX-siRNA1: forward 5′–GGAAGAAAUCGCUGAGGAATT–3′, reverse 5′–UUCCUCAGCGAUUUCUUCCTT–3′; CAIX-siRNA2: forward 5′–GCAACAAUGGCCACAGUGUTT–3′, reverse 5′–ACACUGUGGCCAUUGUUGCTT–3′; CAIX-siRNA3: forward 5′–CCAGUCCAGCUGAAUUCCUTT–3′, reverse 5′–AGGAAUUCAGCUGGACUGGTT–3′. RNase-free diethyl pyrocarbonate-treated Milli-Q water was used for all dilutions.

siRNA (sense, 5′-GCAGACCUCUUCUACUCUUTT-3′ and antisense, 5′-AAGAGUAGAAGAGGUCUGCTT-3′) specific to human MRP-1 and negative control (NC) siRNA (sense, 5′-UUCUCCGAACGUGUCACGUTT-3′ and antisense, 5′-ACGUGACACGUUCGGAGAATT-3′) and FAM-labeled siRNA (FAM-siRNA) were purchased from Gene Pharma Co. Ltd. (Shanghai, China). Fluorescein isothiocyanate (FITC) and DOX was obtained from Aladdin Co. Ltd. (Shanghai, China). PLLA was purchased from Jinan Daigang Biomaterial Co. Ltd. (Jinan, China). CS, sodium tripolyphosphate (TPP), Pluronic F-127 (PF-127), ammonium bicarbonate (AB), and dichloromethane (DCM) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). CO₂ (purity >99.9%, v/v) was supplied by Xiamen Rihong Co., Ltd. (Xiamen, China). Roswell Park Memorial Institute medium (RPMI-1640), fetal bovine serum (FBS), and dim-ethyl sulfoxide (DMSO) were obtained from GIBCO/BRL Life Technologies (Grand Island, NY, USA). 4′6-Diamidino-2-phenylindole (DAPI) and LysoTracker Red were obtained from KeyGen BioTECH Co. Ltd (Jiangsu, China).