Goat anti tnf α

Manufactured by Santa Cruz Biotechnology

Sourced in United Kingdom

Goat anti-TNF-α is a laboratory reagent used for the detection and quantification of tumor necrosis factor alpha (TNF-α) in biological samples. It is a polyclonal antibody raised in goats against recombinant human TNF-α.

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Lab products found in correlation

Rat anti f4 80, by Bio-Rad (1 mentions) Rabbit anti mif, by Santa Cruz Biotechnology (1 mentions) Goat anti cd74, by Santa Cruz Biotechnology (1 mentions) Peroxidase labeled secondary antibody, by Agilent Technologies (1 mentions) Gel loading buffer, by New England Biolabs (1 mentions) Rabbit anti atf3, by Cell Signaling Technology (1 mentions) Rabbit anti stat1, by Abcam (1 mentions) Rabbit anti atf2, by Cell Signaling Technology (1 mentions) Clarity western ecl substrate, by Bio-Rad (1 mentions) Goat anti gapdh, by Santa Cruz Biotechnology (1 mentions) Rabbit anti troponin 1, by Abcam (1 mentions) T per tissue protein extraction buffer, by Thermo Fisher Scientific (1 mentions) M per mammalian protein extraction buffer, by Thermo Fisher Scientific (1 mentions) Goat anti gfap, by Santa Cruz Biotechnology (1 mentions) Biotinylated anti rabbit igg, by Santa Cruz Biotechnology (1 mentions) Rabbit anti caspase 3, by Santa Cruz Biotechnology (1 mentions) Rabbit anti fas, by Santa Cruz Biotechnology (1 mentions) Bx53 microscope, by Olympus (1 mentions) Dp73 camera, by Olympus (1 mentions) Mab377, by Merck Group (1 mentions)

6 protocols using goat anti tnf α

Immunohistochemical Analysis of Kidney Inflammation

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Immunohistochemistry was performed on paraffin embedded slides for renal MIF, CD74 expression, pro‐inflammatory cytokines as MCP‐1 and TNF‐α expression, and for infiltration of neutrophils, F4/80⁺ macrophages and CD3⁺ T cells as described previously.9, 11, 22, 23, 24 The catalogue number of the antibodies for immunohistochemistry as following: rabbit anti‐MIF (Santa Cruz, sc‐20121), goat anti‐CD74 (Santa Cruz, sc‐5438), goat anti‐MCP‐1 (Santa Cruz, sc‐1785), goat anti‐TNF‐α (Santa Cruz, sc‐1351), rabbit anti‐neutrophil (Santa Cruz, sc‐59338), rat anti‐F4/80 (Serotec, MCA497) and rabbit anti‐CD3 (Abcam, ab‐16669).

Li J.H., Tang Y., Lv J., Wang X.H., Yang H., Tang P.M., Huang X.R., He Z.J., Zhou Z.J., Huang Q.Y., Klug J., Meinhardt A., Fingerle‐Rowson G., Xu A.P., Zheng Z.H, & Lan H.Y. (2019). Macrophage migration inhibitory factor promotes renal injury induced by ischemic reperfusion. Journal of Cellular and Molecular Medicine, 23(6), 3867-3877.

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Immunoblotting Analysis of MNV Infection

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Immunoblotting analysis was performed as described in reference 23 (link). Briefly, approximately 2.10⁶ RAW264.7 cells were plated onto 35-mm dishes and infected the next day with MNV at an MOI of 10 as described above. At the indicated times, cells were lysed in 150 μl of 1× gel loading buffer (New England BioLabs), sonicated, and boiled 5 min at 95°C. Cell lysates were separated by SDS-PAGE using 10 μg of total proteins, and the proteins were transferred to polyvinylidene difluoride membranes. These were then probed with the following primary antibodies: mouse anti-phospho-ATF2 T71 (1:1,000; Abcam), rabbit anti-ATF3 (1:500; Cell Signaling), goat anti-TNF-α (1:500; Santa Cruz), rabbit anti-Ddx58 (1:500; Santa Cruz; sc-376845), rabbit anti-Stat1 (1:1,000; Abcam; Ab92506), rabbit anti-ATF2 (1:1,000; Cell Signaling; 35031), mouse anti-VP1 (1:500; I. G. Goodfellow, Cambridge, UK), anti-MNV-3 mouse immune sera (1:1,000; I. G. Goodfellow, Cambridge, UK), rabbit anti-NS7 (1:10,000; I. G. Goodfellow, Cambridge, UK), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Clone 6C5, 1:20,000; Invitrogen), followed by incubation with the appropriate peroxidase-labeled secondary antibodies (Dako) and chemiluminescence development using the Clarity Western ECL Substrate (Bio-Rad). The results were acquired using the Vilber imaging system.

Brocard M., Lu J., Hall B., Borah K., Moller-Levet C., Georgana I., Sorgeloos F., Beste D.J., Goodfellow I.G, & Locker N. (2021). Murine Norovirus Infection Results in Anti-inflammatory Response Downstream of Amino Acid Depletion in Macrophages. Journal of Virology, 95(20), e01134-21.

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Protein Lysate Extraction and Western Blot Analysis

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Heart protein lysates and cellular protein lysates were prepared using T-PER tissue protein extraction buffer and M-PER mammalian protein extraction buffer (Thermo Scientific), respectively. Rabbit anti-phosphor-ERK, goat anti-total ERK, goat anti-TNF-α and goat anti-GAPDH antibodies were purchased from Santa Cruz Biotechnology, whereas rabbit anti-phosphor-AKT and anti-GSK3β antibodies were purchased from Cell Signaling Technology. The rabbit anti-FABP4 from Cayman and the rabbit anti-Troponin I from Abcam were used in Immunofluorescence and Western blots.

Zhang J., Qiao C., Chang L., Guo Y., Fan Y., Villacorta L., Chen Y.E, & Zhang J. (2016). Cardiomyocyte Overexpression of FABP4 Aggravates Pressure Overload-Induced Heart Hypertrophy. PLoS ONE, 11(6), e0157372.

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Immunohistochemical Analysis of Neuroinflammation

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Standard procedures were used, as previously described [31 (link)], on randomly selected sections (6 μm-thick) made on paraffin-embedded brain right hemispheres, in the coronal plane. Primary antibodies used were goat anti-NeuN (mature neuron marker, 1:1000 dilution), goat anti-GFAP (astrocyte marker, 1:100 dilution), goat anti-iba1/CD68 (microglial marker, 1:1000 dilution), goat anti-TNF-α (pro-inflammatory cytokine, 1:500 dilution), rabbit anti-Fas (apoptosis mediator, 1:500 dilution), rabbit anti-Fas ligand (death receptor, 1:500 dilution), and rabbit anti-caspase 3 (marker of apoptosis, 1:500 dilution) (Santa Cruz Biotechnology, CA). Biotinylated anti-goat IgG and biotinylated anti-rabbit IgG (1:200 dilution, Santa Cruz Biotechnology, CA) were the secondary antibodies. The chromogen substrate 3,3′-diaminobenzidine hydrochloride (DAB) was applied and counterstained with hematoxylin. Glass coverslips were placed using DPX mounting medium. Sections labeled were observed with a computerized light microscope including an Olympus BX53 microscope equipped with an Olympus DP73 camera (Olympus, Tokyo, Japan), under 4×, 20×, 40× and 100× objectives.

Seke Etet P.F., Farahna M., Khayr M.A., Omar K.M., Deniz Ö.G., Mustafa H.N., Alatta N.O., Alhayani A., Kaplan S, & Vecchio L. (2017). Evaluation of the safety of conventional lighting replacement by artificial daylight. Journal of Microscopy and Ultrastructure, 5(4), 206-215.

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Antibody Characterization for Neurological Study

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The custom anti-Trpm4 and anti-Sur1 antibodies used for this study have been previously described (15 (link)). The antigenic peptide for Trpm4 was the N-terminal intracellular domain of mouse Trpm4, corresponding to amino acids 1–612 (NP 780339). Anti-Trpm4 antibodies were raised in chicken, and were used at 1:200 dilution. The antigenic peptide for Sur1 was the intra-cellular nucleotide-binding domain 1 of Sur1 (rat Sur1 cDNA amino acids 598 to 965 of NP_037171). Anti-Sur1 antibodies were raised in rabbit and were used at 1:200 dilution. Other primary antibodies included: rabbit anti-cytokeratin 20 (prediluted; Ventana, Tucson, AZ) for colonic epithelial cells; mouse anti-glial fibrillary acidic protein (GFAP) (1:500; CY3 conjugated; C-9205; Sigma, St. Louis, MO) for astrocytes; mouse anti-NeuN (1:100; MAB377; Chemicon, Temecula, CA) for neurons; goat anti-CD31 (PECAM-1) (1:200; sc-1506; Santa Cruz Biotechnology, Santa Cruz, CA) for endothelial cells; goat anti-IgG (1:500; FITC conjugated; NB7477; Novus Biologicals, Littleton, CO) for immunoglobulin G (IgG); goat anti-TNFα (1:100; sc-1350; Santa Cruz Biotechnology, Santa Cruz, CA) for tumor necrosis factor α; rabbit anti-myeloperoxidase (MPO) (1:200, A0398; Dako, Carpinteria, CA) for neutrophils; and mouse anti-rat endothelial cell antigen (Reca-1) (1:100; MA1-81510; Thermo Fisher, Rockford, IL) for rat endothelium.

Mehta R.I., Tosun C., Ivanova S., Tsymbalyuk N., Famakin B.M., Kwon M.S., Castellani R.J., Gerzanich V, & Simard J.M. (2015). Sur1-Trpm4 Cation Channel Expression in Human Cerebral Infarcts. Journal of neuropathology and experimental neurology, 74(8), 835-849.

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Immunohistochemical Analysis of Immune Markers

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Sections were treated with 0.3% H₂O₂ in methanol and then were treated with 10% normal donkey serum for 2 h at 24°C. The sections were incubated with the mouse anti-W3/25 (1 : 200; Harlan Sera-Lab Lid, Loughborough, England), goat anti-IL-4 (1 : 100; BD PharMingen), goat anti-IFN-γ (1 : 100; Santa Cruz Biotechnology), goat anti-ED1 (1 : 500; Santa Cruz Biotechnology), goat anti-TNF-α (1 : 100; Santa Cruz Biotechnology), and goat anti-IL-10 (1 : 100; Santa Cruz Biotechnology) antibodies in a humidified chamber overnight at 4°C. After being washed with cold PBS, the sections were incubated with biotinylated-anti-mouse secondary antibodies (1 : 200; Jackson ImmunoResearch) or biotinylated-anti-goat secondary antibodies (1 : 200; Jackson ImmunoResearch) for 2 h at 24°C and finally with peroxidase conjugated streptavidin (1 : 300; Dako) for 1 h at 24°C. The peroxidase was developed using 3,30-diaminobenzidine (DAB substrate kit; Vector Laboratories), and then the samples were counterstained with Mayer's hematoxylin.

Zhang X., Wu Z., Liu Y., Ni J., Deng C., Zhao B., Nakanishi H., He J, & Yan X. (2017). Boi-ogi-to (TJ-20), a Kampo Formula, Suppresses the Inflammatory Bone Destruction and the Expression of Cytokines in the Synovia of Ankle Joints of Adjuvant Arthritic Rats. Evidence-based Complementary and Alternative Medicine : eCAM, 2017, 3679295.

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