Log In Sign up
The largest database of trusted experimental protocols

Prosense 750

Manufactured by PerkinElmer
Visit Supplier

The ProSense 750 is a versatile benchtop instrument designed for performing various analytical techniques. It features advanced optics and detection capabilities, enabling users to analyze a wide range of samples with precision and accuracy.

Automatically generated - may contain errors

Lab products found in correlation

Neutrophil elastase 680, by PerkinElmer (2 mentions) Recombinant human bmp 2, by R&D Systems (1 mentions) Osteosense 680, by PerkinElmer (1 mentions) Ivis lumina xr, by PerkinElmer (1 mentions) Mmpsense 680, by PerkinElmer (1 mentions) Prosense 680, by PerkinElmer (1 mentions)

Close spelling variants of this product

ProSense 750-fluorescence agents ProSense 750 FAST

6 protocols using prosense 750

1

Fluorescent Imaging Agent Procurement

Check if the same lab product or an alternative is used in the 5 most similar protocols
Near-infrared fluorescent imaging agent ProSense 750 (NEV10001EX) was purchased from PerkinElmer (Waltham, MA). Iron Dextran was obtained from Watson Pharma, Inc. (Morristown, NJ). Dextran was purchased from Sigma-Aldrich (Saint Louis, MO).
Malhotra R., Wunderer F., Barnes H.J., Bagchi A., Buswell M.D., O’Rourke C.D., Slocum C.L., Ledsky C.D., Peneyra K.M., Sigurslid H., Corman B., Johansson K.B., Rhee D.K., Bloch K.D, & Bloch D.B. (2019). Hepcidin Deficiency Protects Against Atherosclerosis. Arteriosclerosis, thrombosis, and vascular biology, 39(2), 178-187.
+ Open protocol
+ Expand
2

Synthesis and Characterization of LDN-193189

Check if the same lab product or an alternative is used in the 5 most similar protocols
LDN-193189 (4-[6-(4-piperazin-1-ylphenyl)pyrazolo[1,5-a]pyrimidin-3-yl]quinoline) was synthesized as previously described [44 (link)], dissolved in water at a concentration of 0.5 mg/mL, and titrated to a pH of 5.5 with NaOH. Recombinant ALK3-Fc was provided by Acceleron Pharma Inc. (Cambridge, MA). OsteoSense-680 and ProSense-750 were obtained from PerkinElmer (Waltham, MA). Recombinant human BMP-2 was purchased from R&D Systems (Minneapolis, MN).
Malhotra R., Burke M.F., Martyn T., Shakartzi H.R., Thayer T.E., O’Rourke C., Li P., Derwall M., Spagnolli E., Kolodziej S.A., Hoeft K., Mayeur C., Jiramongkolchai P., Kumar R., Buys E.S., Yu P.B., Bloch K.D, & Bloch D.B. (2015). Inhibition of Bone Morphogenetic Protein Signal Transduction Prevents the Medial Vascular Calcification Associated with Matrix Gla Protein Deficiency. PLoS ONE, 10(1), e0117098.
+ Open protocol
+ Expand
3

In Vivo Imaging of Molecular Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two activatable-fluorescent agents were used, ProSense 750 and Neutrophil Elastase 680 (PerkinElmer, Waltham, MA). Animals were imaged using an IVIS Spectrum system. Acquired images were analyzed using LivingImage 4.4 (Caliper Life Sciences). See Supplemental Information online for details.
Perez C.J., Mecklenburg L., Jaubert J., Santamaria L.M., Iritani B.M., Espejo A., Napoli E., Song G., del Río M., DiGiovanni J., Giulivi C., Bedford M.T., Dent S.Y., Wood R.D., Kusewitt D.F., Guénet J.L., Conti C.J, & Benavides F. (2015). Increased Susceptibility to Skin Carcinogenesis Associated with a Spontaneous Mouse Mutation in the Palmitoyl Transferase Zdhhc13 gene. The Journal of investigative dermatology, 135(12), 3133-3143.
+ Open protocol
+ Expand
4

Ex vivo Fluorescence Imaging of Organs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ex vivo fluorescence was evaluated by applying the method reported previously [23 (link)]. Briefly, mice were anesthetized with isoflurane/oxygen/nitrogen, and the aorta, liver, and lung were excised and subsequently underwent fluorescent signal analysis via imaging performed using an in vivo imaging system (IVIS Lumina XR; PerkinElmer Inc.). The fluorescent intensity of ProSense 750 (PerkinElmer Inc.) in the selected organ was analyzed.
Kim K.M., Lee J.Y., Jeon B.H., Quan K.T., Na M., Nam K.W, & Chae S. (2021). Extract of Curcuma zedoaria R. prevents atherosclerosis in apolipoprotein E-deficient mice. Nutrition Research and Practice, 15(3), 319-328.
+ Open protocol
+ Expand
5

Enzyme-Activated Fluorescent Imaging Probes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The commercially available enzyme activatable NIRF imaging probes (PerkinElmer) listed in Table 1. were given via retro orbital injection. All probes are optically silent until cleaved and activated by proteases. The fluorophore on 680 series probes is excited by light at 680±10 nm and emits light at 700±10nm. The fluorophore on 750 series probes is excited at 750nm and emits at 770nm. ProSense 680, ProSense 750 and MMPSense 680 were given to animals 24 hours before imaging according to manufacture instructions (PerkinElmer). FAST (Fluorescent Activatable Sensor Technology) imaging agents designed to permit earlier imaging were given 6 hours before imaging as per manufacture instructions (PerkinElmer). Each probe was diluted in 1X phosphate buffered saline (1X PBS) before injection and at doses recommended by the manufacturer. Published reports indicate up-regulation of both cathepsins and MMPs in damaged and acutely inflamed colon of DSS treated mice (12 (link), 13 (link)). The peptide substrate of ProSense 680 is activated by proteases such as cathepsin B, L, S and plasmin with reportedly highest specificity for cathepsin B (21 (link)). MMPSense substrate is cleaved by key MMPs including MMP-2, -3, -9 and -13 with reportedly highest specificity for MMP-9 (according to manufacturer).
Ding S., Blue R.E., Morgan D.R, & Lund P.K. (2014). Comparison of multiple enzyme activatable near infrared fluorescent molecular probes for detection and quantification of inflammation in murine colitis models. Inflammatory bowel diseases, 20(2), 363-377.
+ Open protocol
+ Expand
6

In Vivo Imaging of Molecular Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two activatable-fluorescent agents were used, ProSense 750 and Neutrophil Elastase 680 (PerkinElmer, Waltham, MA). Animals were imaged using an IVIS Spectrum system. Acquired images were analyzed using LivingImage 4.4 (Caliper Life Sciences). See Supplemental Information online for details.
Perez C.J., Mecklenburg L., Jaubert J., Santamaria L.M., Iritani B.M., Espejo A., Napoli E., Song G., del Río M., DiGiovanni J., Giulivi C., Bedford M.T., Dent S.Y., Wood R.D., Kusewitt D.F., Guénet J.L., Conti C.J, & Benavides F. (2015). Increased Susceptibility to Skin Carcinogenesis Associated with a Spontaneous Mouse Mutation in the Palmitoyl Transferase Zdhhc13 gene. The Journal of investigative dermatology, 135(12), 3133-3143.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!