Anti cd40 hm40 3 agonistic antibody

Manufactured by Thermo Fisher Scientific

The Anti-CD40 (HM40-3) agonistic antibody is a laboratory reagent used for cell biology research. It binds to and activates the CD40 receptor, which is expressed on the surface of various cell types. This antibody can be used to study the role of CD40 signaling in cellular processes.

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Lab products found in correlation

Magnisort mouse b cell enrichment kit, by Thermo Fisher Scientific (2 mentions) Anti b220 antibody, by BioLegend (2 mentions) Mil 4, by R&D Systems (2 mentions) Lipopolysaccharides (lps), by Merck Group (2 mentions) Facscalibur, by BD (2 mentions) Cellquest, by BD (1 mentions) Tgf β, by Thermo Fisher Scientific (1 mentions) Retinoic acid, by Merck Group (1 mentions) Cellquest software, by BD (1 mentions)

Close spelling variants of this product

Anti-CD40 (HM40-3; HM40-3 Anti-CD40 antibody Anti-CD40

2 protocols using anti cd40 hm40 3 agonistic antibody

Purification and Stimulation of Murine Splenic B Cells

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Mouse splenic B cells were purified by negative selection (eBioscience Magnisort Mouse B cell enrichment kit). B-cell purity was >95% as measured by FACS analysis (FACSCalibur and CellQuest software; BD Biosciences) using anti-B220 antibody (BioLegend). Purified B cells were seeded at a final concentration of 0.5 or 0.25 × 10⁶ cells/mL. For plasmablast differentiation, B cells were stimulated with 5 µg/mL LPS (Sigma) for 72 h, and for IgG1 CSR, B cells were stimulated 5 µg/mL anti-CD40 (HM40-3) agonistic antibody (eBioscience), or 5 µg/mL LPS (Sigma), together with 5 ng/mL mIL-4 (R&D Systems) for 96 h. All B cells were cultured in RPMI 1640 supplemented with 10% (vol/vol) heat-inactivated FCS, 5 mM Hepes, 2 mM l-glutamine, 100 U/mL penicillin, 100 µg/mL streptomycin, 50 µM 2-mercaptoethanol.

Chiu H., Mallya S., Nguyen P., Mai A., Jackson L.V., Winkler D.G., DiNitto J.P., Brophy E.E., McGovern K., Kutok J.L, & Fruman D.A. (2017). The Selective Phosphoinoside-3-Kinase p110δ Inhibitor IPI-3063 Potently Suppresses B Cell Survival, Proliferation, and Differentiation. Frontiers in Immunology, 8, 747.

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Induction of Immunoglobulin Class Switching in Murine B Cells

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Splenic B cells were purified by negative selection (eBioscience Magnisort Mouse B cell enrichment kit). B cell purity measured by FACS analysis (FACSCalibur and CellQuest software; BD Biosciences) using anti-B220 antibody (Biolegend) yielded >95% B220+. Purified B cells were seeded at a final concentration of 1.5 × 10^6 cells per milliliter. For class switching experiments, B cells were stimulated 5 μg/mL anti-CD40 (HM40–3) agonistic antibody (eBioscience), or 5 μg/mL LPS (Sigma), together with 5 ng/mL mIL-4 (R&D Systems) for 96 h to induce switching to IgG1. 5 μg/mL LPS (Sigma) induced switching to IgG3. 5 μg/mL LPS (Sigma) with 5 ng/mL mIL-4 (R&D), 5 ng/mL mIL-5 (Tonbo Bioscience), 5 nM retinoic acid (Sigma Aldrich) and 5 ng/mL TGF-β (Thermo Fisher Scientific) induced switching to IgA. All cells were cultured with RPMI 1640 supplemented with 10% (vol/vol) heat-inactivated FCS, 5 mM Hepes, 2 mM L-glutamine, 100 units/mL penicillin, 100 μg/mL streptomycin, 50 μM 2-mercaptoethanol.

Chiu H., Jackson L.V., Oh K.I., Mai A., Ronai Z.A., Ruggero D, & Fruman D.A. (2018). The mTORC1/4E-BP/eIF4E Axis Promotes Antibody Class Switching in B Lymphocytes. Journal of immunology (Baltimore, Md. : 1950), 202(2), 579-590.

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