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Caspase 8 cleaved mouse bid c8 bid

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Caspase-8 cleaved mouse BID (C8-BID) is a recombinant protein that represents the cleaved form of the BID protein from mouse origin. BID is a pro-apoptotic member of the Bcl-2 family that is involved in the mitochondrial apoptosis pathway. Caspase-8 cleaves BID, generating the C8-BID fragment which can then translocate to the mitochondria and induce cytochrome c release and apoptosis.

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Lab products found in correlation

Cell culture and transfection reagents, by Thermo Fisher Scientific (2 mentions) Anti bad, by Merck Group (2 mentions) Anti puma, by Merck Group (2 mentions) Anti bcl 2, by BD (2 mentions) Anti bak, by Merck Group (2 mentions) Abt 263 plx 4032 gsk 110212, by Selleck Chemicals (2 mentions) Staurosporine cisplatin dacarbazine vinblastine, by Merck Group (2 mentions) Anti a1 fl 175, by BD (2 mentions) Anti mcl 1, by Rockland Immunochemicals (2 mentions) Anti bim, by Merck Group (2 mentions) Anti smac, by Merck Group (2 mentions) Anti hsp60 b 9, by Merck Group (2 mentions) Anti p44 p42 mapk erk1 2 137f5, by Merck Group (2 mentions) Anti phospho p44 42 mapk erk1 2 197g2, by Merck Group (2 mentions) Human bim bh3 domain peptide, by Abcepta (2 mentions) Hoechst 33342, by AnaSpec (2 mentions) Anti gapdh, by Merck Group (2 mentions) Cytochrome c, by Merck Group (2 mentions) Anti actin, by BD (2 mentions) Anti cd31, by BD (2 mentions)

2 protocols using caspase 8 cleaved mouse bid c8 bid

1

Apoptotic Signaling Pathway Analysis

Cited 1 time
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All cell culture and transfection reagents were from Invitrogen; and standard reagents were from Sigma or Fisher Scientific. Drugs were from: ABT-737 (Abbott Pharmaceuticals), ABT-263/PLX-4032/GSK-110212 (Selleck), zVAD-fmk (Calbiochem), Hoechst 33342 (Anaspec), and staurosporine/cisplatin/dacarbazine/vinblastine (Sigma). Antibodies (clone): anti-actin (C4), anti-A1 (FL-175), anti-BCL-2 (100), anti-CD31 (BD Pharmingen #550274) anti-MCL-1 (Rockland), anti-BAD (C7), anti-BIM (22 (link)–40), anti-PUMA (CT; Sigma), anti-SMAC (H177), anti-BID (C20), anti-cytochrome c (7H8.2C12), anti-BAK (G23), anti-BAX (6A7 for IP; N20 for western blot), anti-BCL-xL (H5 for IP; S18 for western blot), anti-GAPDH (9B3), anti-HSP60 (B-9), anti-p44/p42 MAPK ERK1/2 (137F5), and anti-phospho p44/42 MAPK ERK1/2 (197G2). Caspase-8 cleaved mouse BID (C8-BID) was from R&D Systems. Full-length BAX was made as described (24 (link)). Human BCL-xLΔC, MCL-1ΔC, and PUMAβ were made as described (17 (link)). The human BIM BH3 domain peptide (> 98% purity, Abgent) was resuspended in anhydrous DMSO in a N2 environment, stored at −80°C, and thawed only once. All lipids for the LUVs studies were purchased from Avanti Polar Lipids. Statistical significance was evaluated by two tailed Student t test for p < 0.05.
Serasinghe M.N., Missert D.J., Asciolla J.J., Wieder S.Y., Podgrabinska S., Izadmehr S., Belbin G., Skobe M, & Chipuk J.E. (2014). Anti-apoptotic BCL-2 proteins govern cellular outcome following B-RAFV600E inhibition and can be targeted to reduce resistance. Oncogene, 34(7), 857-867.
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2

Apoptotic Signaling Pathway Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All cell culture and transfection reagents were from Invitrogen; and standard reagents were from Sigma or Fisher Scientific. Drugs were from: ABT-737 (Abbott Pharmaceuticals), ABT-263/PLX-4032/GSK-110212 (Selleck), zVAD-fmk (Calbiochem), Hoechst 33342 (Anaspec), and staurosporine/cisplatin/dacarbazine/vinblastine (Sigma). Antibodies (clone): anti-actin (C4), anti-A1 (FL-175), anti-BCL-2 (100), anti-CD31 (BD Pharmingen #550274) anti-MCL-1 (Rockland), anti-BAD (C7), anti-BIM (22 (link)–40), anti-PUMA (CT; Sigma), anti-SMAC (H177), anti-BID (C20), anti-cytochrome c (7H8.2C12), anti-BAK (G23), anti-BAX (6A7 for IP; N20 for western blot), anti-BCL-xL (H5 for IP; S18 for western blot), anti-GAPDH (9B3), anti-HSP60 (B-9), anti-p44/p42 MAPK ERK1/2 (137F5), and anti-phospho p44/42 MAPK ERK1/2 (197G2). Caspase-8 cleaved mouse BID (C8-BID) was from R&D Systems. Full-length BAX was made as described (24 (link)). Human BCL-xLΔC, MCL-1ΔC, and PUMAβ were made as described (17 (link)). The human BIM BH3 domain peptide (> 98% purity, Abgent) was resuspended in anhydrous DMSO in a N2 environment, stored at −80°C, and thawed only once. All lipids for the LUVs studies were purchased from Avanti Polar Lipids. Statistical significance was evaluated by two tailed Student t test for p < 0.05.
Serasinghe M.N., Missert D.J., Asciolla J.J., Wieder S.Y., Podgrabinska S., Izadmehr S., Belbin G., Skobe M, & Chipuk J.E. (2014). Anti-apoptotic BCL-2 proteins govern cellular outcome following B-RAFV600E inhibition and can be targeted to reduce resistance. Oncogene, 34(7), 857-867.
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