SEC experiments were performed using a NanoAcquity ultra-high pressure LC system (Waters) coupled to a high-resolution maXis II quadrupole time-of-flight mass spectrometer (Bruker Daltonics). 1 μg of total protein was injected onto a PolyHYDROXYETHYL A column (PolyHEA) (PolyLC Inc), 2.1 mm internal diameter, 100 mm length, 5 μm particle size, and 200 Å pore size. Protein samples were separated isocratically with with 200 mM ammonium acetate solution at a flow rate of 28 μL/min for 10 min with a ‘divert to waste’ step programmed at 7 min.
High resolution maxis 2 quadrupole time of flight mass spectrometer
Manufactured by Bruker
The high-resolution maXis II quadrupole time-of-flight mass spectrometer is a laboratory instrument designed for precise mass analysis of chemical compounds. It utilizes a quadrupole mass filter and a time-of-flight mass analyzer to provide high-resolution, accurate mass measurements.
Automatically generated - may contain errors
4 protocols using high resolution maxis 2 quadrupole time of flight mass spectrometer
1
SEC-based Protein Separation by LC-QTOF
2
High-Resolution SEC-MS Protein Separation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SEC experiments were performed using a NanoAcquity ultra-high pressure LC system (Waters) coupled to a high-resolution maXis II quadrupole time-of-flight mass spectrometer (Bruker Daltonics). 1 µg of total protein (n = 3) was injected onto a PolyHYDROXYETHYL A column (PolyHEA) (PolyLC Inc), 2.1 mm internal diameter, 100 mm length, 5 µm particle size, and 200 Å pore size. Protein samples were separated isocratically with 200 mM ammonium acetate solution at a flow rate of 28 µL/min for 10 min with a ‘divert to waste’ step programmed at 7 min.
3
Top-down RPLC-MS/MS Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Top-down RPLC-MS/MS was carried out by either using an Acquity ultra-high pressure LC M-class system (Waters) coupled to a high-resolution maXis II quadrupole time-of-flight (QTOF) mass spectrometer (Bruker Daltonics) or by using a nanoAcquity ultra-high pressure LC system (Waters) coupled to a high-resolution Impact II QTOF mass spectrometer (Bruker Daltonics). 600 ng of total protein was injected onto a home-packed PLRP column (PLRP-S) (Agilent Technologies), 10-μm particle size, 500-μm inner diameter, 1,000 Å pore size using an organic gradient of 20 to 65% mobile phase B (mobile phase A: 0.2% FA in H2O; mobile phase B: 0.2% FA in 50:50 acetonitrile/isopropanol) at a constant flow rate of 12 μL/min.
4
Top-down Proteomics by RPLC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Top-down RPLC-MS/MS was carried out by either using an Acquity ultra-high pressure LC M-class system (Waters) coupled to a high-resolution maXis II quadrupole time-of-flight (QTOF) mass spectrometer (Bruker Daltonics) or by using a nanoAcquity ultra-high pressure LC system (Waters) coupled to a high-resolution Impact II QTOF mass spectrometer (Bruker Daltonics). 600 ng of total protein (n = 3) was injected onto a home-packed PLRP column (PLRP-S) (Agilent Technologies), 10-µm particle size, 500-µm inner diameter, 1,000 Å pore size using an organic gradient of 20 to 65% mobile phase B (mobile phase A: 0.2% FA in H2O; mobile phase B: 0.2% FA in 50:50 acetonitrile/isopropanol) at a constant flow rate of 12 μL/min.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!