We obtained Gad2-IRES-Cre (Taniguchi et al., 2011 (link), Jax stock #010802), Scnn1a-tg3-cre (Madisen et al., 2010 (link), Jax stock #009613), and Ai14 mice (Madisen et al., 2010 (link), Jax stock #007914) from the Jackson Laboratory. These mice were crossed to generate Gad2-Ai14 and Scnn1a-Ai14 mice. Both sexes were used. In Gad2-Ai14 mice, most cortical inhibitory neurons express tdTomato (Taniguchi et al., 2011 (link)). In Scnn1a-Ai14 mice, layer 4 neurons express tdTomato (Madisen et al., 2010 (link)).
Jax stock
Manufactured by Jackson ImmunoResearch
JAX stock is a laboratory product offered by Jackson ImmunoResearch. It is a collection of purified immunoglobulins that can be used as reference standards or controls in immunoassays and other applications. JAX stock is designed to provide consistent and reliable performance in these laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
B6 129p2 pvalbtm1 cre arbr j, by Jackson ImmunoResearch (1 mentions)
Animal environmental control chamber, by Daehan Biolink (1 mentions)
C57bl 6, by Daehan Biolink (1 mentions)
Genomic dna purification kit, by Promega (1 mentions)
Gad2 ires cre, by Jackson ImmunoResearch (1 mentions)
Scnn1a tg3 cre, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Gad2 cre mice, by Jackson ImmunoResearch (1 mentions)
7 protocols using jax stock
1
Genetically Engineered Mice for Neuronal Labeling
2
Generation of Heterozygous PV-Cre Mice
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Male breeders from B6 PV-Cre knock-in homozygous mice line (B6;129P2-Pvalbtm1(cre)Arbr/J, JAX stock #017320, The Jackson Laboratory) were bred with WT C57BL/6J females (JAX stock #000664) to generate an in-house colony of heterozygous PV-Cre C57BL/6J at the University of Cincinnati animal housing facility. The PV-Cre mouse line has been characterized extensively in prior publications (Zhao et al., 2019 (link); Cummings and Clem, 2020 (link); Groisman et al., 2020 (link)). Mice were maintained under standard conditions (12/12 h light/dark cycle, 22 ± 1°C, food and water ad libitum; four mice per cage on arrival) in accordance with the University of Cincinnati Institutional Animal Care and Use Committee, which specifically approved all acute and chronic stress regimens employed in this proposal. Mice were single housed following surgeries and continued to be housed singly throughout the duration of the experiment, to prevent aggression and injury to animals following surgery and during the stress paradigms (Lidster et al., 2019 (link)). Enrichment for housing cages included mouse hut and nestlets. All protocols conformed to the Society’s Policies on the Use of Animals in Neuroscience Research. All experiments were performed on adult male mice (∼7.5 months of age at surgery).
3
PV Interneuron Labeling in Adult Mice
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All experimental procedures were carried out in accordance with institutional animal welfare guidelines, and licensed by the UK Home Office. Experiments were carried out on adult mice (aged >P90), and no systematic randomization was used with respect to region labeled or other conditions. For experiments in which parvalbumin (PV) interneurons were labeled, this was achieved by crossing the B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hom/J and B6;129P2-Pvalbtm1(cre)Arbr/J (Jackson Laboratory, JAX Stock 007914 and 008069, respectively). Mice were housed under normal light conditions (14 h light, 10 h dark) and recordings were made during the light period.
4
Genotyping of WT and TCRδ-/- Mice
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Wild-type (WT) C57BL/6 (Daehan Biolink, Seoul, Korea) and TCRδ−/− mice (9 (link)) (JAX stock #002119, Jackson Lab) were maintained on an 8:16-h light:dark cycle in an animal environmental control chamber (Daehan Biolink). Mice used in this study were 8–20 wk of age. Animal care was performed in accordance with the institutional guidelines set forth by Kangwon National University.
Genomic DNA was isolated using a genomic DNA purification kit according to the manufacturer's protocol (Promega, Madison, WI, USA). To confirm either WT or TCRδ knockout genotype, PCR was performed with 4 primers: TCRδ mutant forward primer, oIMR6916, 5′-CTT GGG TGG AGA GGC TAT TC-3′; reverse primer, oIMR6917, 5′-AGG TGA GAT GAC AGG AGA TC-3′; internal positive control forward primer, oIMR8744, 5′-CAA ATG TTG CTT GTC TGG TG-3′; and reverse primer, oIMR8745, 5′-GTC AGT CGA GTG CAC AGT TT-3′. The WT and TCRδ knockout alleles resulted in a 200 bp and 280 bp product, respectively.
Genomic DNA was isolated using a genomic DNA purification kit according to the manufacturer's protocol (Promega, Madison, WI, USA). To confirm either WT or TCRδ knockout genotype, PCR was performed with 4 primers: TCRδ mutant forward primer, oIMR6916, 5′-CTT GGG TGG AGA GGC TAT TC-3′; reverse primer, oIMR6917, 5′-AGG TGA GAT GAC AGG AGA TC-3′; internal positive control forward primer, oIMR8744, 5′-CAA ATG TTG CTT GTC TGG TG-3′; and reverse primer, oIMR8745, 5′-GTC AGT CGA GTG CAC AGT TT-3′. The WT and TCRδ knockout alleles resulted in a 200 bp and 280 bp product, respectively.
5
Genetic Mouse Models for Taste and Immune Research
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All experiments were conducted according to the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals, and protocols were approved by the University of Miami Institutional Animal Care and Use Committee. Mice of the following strains (Jax stock #) were purchased from The Jackson Laboratory and bred in-house: C57BL/6J (#000664), Mafb-2A-mCherry-2A-Cre (#029664), and Penk-IRES2-Cre (#025112). The Mafb-2A-mCherry-2A-Cre mice express both Cre and mCherry in many circulating and tissue-resident immune cells (X. Wu et al., 2016 (link)) as well as in a subset of neurons in the geniculate ganglion (Dvoryanchikov et al., 2017 (link)). Penk-IRES2-Cre mice express Cre in enkephalinergic neurons of the brain and spinal cord (François et al., 2017 (link); Daigle et al., 2018 (link)) and also in the T3 subset of gustatory neurons of the geniculate ganglion (Dvoryanchikov et al., 2017 (link)). Mice of the Plcb2-GFP strain were produced and bred in-house and express GFP in the Type II cells found within all taste buds (Kim et al., 2006 (link)).
6
Conditional Expression of hAGO2 in Oocytes
7
Choline acetyltransferase and glutamate decarboxylase 2 expressing mouse models
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We used mice expressing Cre recombinase under control of the choline acetyltransferase (ChAT-Cre mice; JAX Stock #006410, The Jackson Laboratory) or glutamate decarboxylase 2 (GAD2-Cre mice, JAX Stock #010802, The Jackson Laboratory) promotor 49, (link)50 (link) . Animals of either sex were used.
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