Anti armenian hamster igg

Manufactured by Jackson ImmunoResearch

Anti-Armenian Hamster IgG is a laboratory reagent used for detection and analysis purposes. It is a purified antibody that specifically binds to immunoglobulin G (IgG) molecules from Armenian hamsters.

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Lab products found in correlation

Hu fcr binding inhibitor, by Thermo Fisher Scientific (1 mentions) Anti cd14 clone t k4, by Miltenyi Biotec (1 mentions) Anti cd11b m1 70, by BioLegend (1 mentions) Anti f4 80 bm8, by Thermo Fisher Scientific (1 mentions) Streptavidin dynabeads, by Thermo Fisher Scientific (1 mentions) Anti cd28, by BioLegend (1 mentions) Anti cd3e, by BD (1 mentions) Recombinant human il 2, by Thermo Fisher Scientific (1 mentions) Anti cd28, by BD (1 mentions)

Spelling variants of this product

Hamster IgG (6 citations) Goat anti–Armenian hamster IgG (5 citations) Goat anti-Armenian hamster IgG antibodies (3 citations) Goat anti-Armenian hamster IgG Alexa Fluor 594 (2 citations) Cy3-conjugated goat anti-Armenian hamster IgG (2 citations) Peroxidase AffiniPure goat anti-Armenian hamster IgG (H+L) (2 citations)

3 protocols using anti armenian hamster igg

Comprehensive Immune Cell Profiling Protocol

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RPMI 1640 was obtained from Corning Technologies. Antibiotics were
purchased from Sigma. FBS purchased from Hyclone. Dead cells were excluded using
Invitrogen Fixable LIVE/DEAD in Near-IR, Yellow, or Violet. The following
antibodies were used for flow cytometry or immunofluorescence staining: from
BioLegend: anti-VISTA (clone MH5A), anti-CD45 (30-F11), anti-CD11b (M1/70),
anti-CD4 (RM4–5), anti-CD8 (53–6.7), anti-Ly6C (HK1.4), anti-Ly6G
(IA8), anti-Gr1 (RB6–8C5), anti-F4/80 (BM8), and Armenian Hamster IgG
Isotype control (HTK888); from eBioscience: anti-CD11c (N418), anti-CD16/CD32
(clone 93), and anti-FoxP3 (FJK-16s); anti- Armenian Hamster IgG (Jackson
ImmunoResearch), and anti-VISTA (clone 13F3, made in-house). Antibodies for flow
cytometry staining of human PBMCs: Hu FcR Binding Inhibitor (eBioscience),
anti-VISTA (GG8, made in-house), anti-CD14 (clone TÜK4, Miltenyi), and
from BioLegend: anti-CD11b (M1/70), anti-CD33 (WM-53), anti-HLA-DR (L243),
anti-CD3 (SK7), anti-CD19 (HIB19), and mouse IgG₁ κ isotype
control (MOPC-21). For blocking experiments, antibodies used were: anti-VISTA
(clone 13F3, made in-house) and Armenian Hamster IgG1 Isotype Control (clone
PIP, BioXCell).

Deng J., Li J., Sarde A., Lines J.L., Lee Y.C., Qian D.C., Pechenick D.A., Manivanh R., LeMercier I., Lowrey C.H., Varn F.S., Cheng C., Leib D.A., Noelle R.J, & Mabaera R. (2019). Hypoxia-induced VISTA promotes the suppressive function of myeloid- derived suppressor cells in the tumor microenvironment. Cancer immunology research, 7(7), 1079-1090.

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Quantification of T and B Cell PIP3 Levels

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CD4 and CD8 cells were isolated from mouse splenocytes using immunomagnetic negative selection by biotinylated cocktail of antibodies and Streptavidin dynabeads (Invitrogen). T cells were stimulated with anti-CD3 (1 mg/mL), (145-2c11, Biolegend) anti-CD28 (2 µg/mL), (35.51, Biolegend) antibodies followed by crosslinking with anti-Armenian hamster IgG (10 µg/mL, Jackson ImmunoResearch labs). Cells were stimulated for 1 min at 37 °C. B cells were similarly isolated from mouse splenocytes and stimulated for 1 min at 37 °C with anti-IgM 2 µg/mL (AffinPure F(ab’)₂ Fragment goat anti-mouse IgM from Jackson Immuno-Research labs).
After stimulation of the cells, we terminated the reactions by addition of 750 µL kill solution (CHCl₃:MeOH:1 M HCL (10:20:1) and immediately froze the samples on dry ice. PIP₃ levels were quantified by mass spectrometry as previously described^{51 (link)}.

Stark A.K., Chandra A., Chakraborty K., Alam R., Carbonaro V., Clark J., Sriskantharajah S., Bradley G., Richter A.G., Banham-Hall E., Clatworthy M.R., Nejentsev S., Hamblin J.N., Hessel E.M., Condliffe A.M, & Okkenhaug K. (2018). PI3Kδ hyper-activation promotes development of B cells that exacerbate Streptococcus pneumoniae infection in an antibody-independent manner. Nature Communications, 9, 3174.

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Stimulating P14 CD8+ T Cells

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24-well plates were coated with anti-Armenian Hamster IgG (Jackson ImmunoResearch
#127-005-099) at 30μg/mL in PBS at 4°C overnight. Purified P14 CD8⁺ T
cells were stimulated at 1x10⁶ cells in 300μL complete RPMI (RPMI
with 10% FBS, Pen/strep, L-glutamine, 50uM β-ME) with 2μg/mL anti-CD3e (BD
#567114), anti-CD28 (BD #567110), and 100U/mL recombinant human IL-2 (PeproTech
#212-02).

McDonald B., Chick B.Y., Hargreaves D.C, & Kaech S.M. (2023). Early Chromatin Remodeling Events in Acutely Stimulated CD8+ T Cells. The Yale Journal of Biology and Medicine, 96(4), 467-473.

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