Ulimate c18 column

One milliliter of each sample was extracted three times with 1 mL diethylether, the ether fractions were combined and then evaporated to dryness under a stream of nitrogen gas and redissolved in 100μL ethanol. The samples were filtered through a 0.4μm filter and stored at 4°C until analysis[10 (link)].
A 20μL sample was separated using a Welch Ulimate C18 column (250 × 4.6 mm, 4μm). The temperature was set to 30°C and the flow rate as maintained at 0.6 mL/min. Elution was isocratic with a mobile phase consisting of methanol: acetonitrile: water (20:30:50). Daidzein and DHD were detected at 260nm.

A 20 μL volume of the sample was injected and separated over a Welch Ulimate C18 column (250 × 4.6 mm, 4 μm). The temperature was set at 30°C, and the flow rate was maintained at 0.6 ml/min. Elution was isocratic with a mobile phase consisting of methanol: acetonitrile: water (20:30:50). Daidzein and DHD were detected at 260 nm. Equol were detected at 205 nm.