Propidium iodide

Manufactured by Vazyme

Sourced in China

Propidium iodide is a fluorescent dye used in flow cytometry and microscopy. It intercalates with DNA and is commonly used to stain and identify dead or dying cells.

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Lab products found in correlation

Annexin 5 fluorescein isothiocyanate fitc, by Vazyme (4 mentions) Annexin 5 fitc, by Vazyme (4 mentions) Annexin 5 fluorescein isothiocyanate, by Vazyme (4 mentions) Facscalibur, by BD (2 mentions) Facscanto 2, by BD (2 mentions) Facscan flow cytometry, by BD (1 mentions) Phosphate buffered saline (pbs), by Solarbio (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Flow cytometry, by BD (1 mentions) Annexin 5 fitc and pi solution, by Vazyme (1 mentions) Annexin 5 fitc and pi, by BD (1 mentions) Pe anti human cd11b, by BioLegend (1 mentions) Cck 8 solution, by Dojindo Laboratories (1 mentions) Crystal violet staining solution, by Beyotime (1 mentions) Novocyte 1300, by Agilent Technologies (1 mentions) Fitc conjugated annexin 5, by Vazyme (1 mentions) Annexin 5, by Vazyme (1 mentions) Annexin 5 fitc pi apoptosis detection kit, by Vazyme (1 mentions) Propidium iodide, by Merck Group (1 mentions) Annexin 5 fitc apoptosis detection kit, by Vazyme (1 mentions)

Spelling variants of this product

Annexin V-FITC/propidium iodide (PI) Apoptosis Detection Kit (17 citations) Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit (5 citations) Annexin V-fluorescein isothiocyanate/propidium iodide apoptosis detection kit (3 citations) Propidium iodide staining solution (2 citations) Annexin V-FITC/propidium iodide (AV/PI) Apoptosis Detection kit (2 citations) Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) kit (2 citations) Annexin V-Fluorescein isothiocyanate (FITC) and propidium iodide (PI) staining kit (2 citations) Annexin V FITC/Propidium Iodide Kit (2 citations) FITC-conjugated Annexin V and propidium iodide (2 citations)

25 protocols using propidium iodide

Cell Apoptosis and Cycle Assay Protocol

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For cell apoptosis determination, the transfected THP-1 and HL-60 cells were collected and washed twice using PBS (Solarbio). Next, the cells were resuspended and then labeled with Annexin V-fluorescein isothiocyanate (FITC; Vazyme, Nanjing, China) and propidium iodide (PI; Vazyme) for 20 min. After washing with PBS (Solarbio), the apoptotic cells were examined by a FACScan^® flow cytometry (BD Bioscience, Franklin Lakes, NJ, USA).
For cell cycle determination, the collected cells were fixed with 70% ethanol overnight at 4°C and then washed using PBS (Solarbio). Then, the cells were suspended and mixed with PI (Vazyme) for 30 min at 37°C. After that, cell cycle was estimated with a FACScan^® flow cytometry (BD Bioscience).

Wang Y., Guo T., Liu Q, & Xie X. (2020). CircRAD18 Accelerates the Progression of Acute Myeloid Leukemia by Modulation of miR-206/PRKACB Axis. Cancer Management and Research, 12, 10887-10896.

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Cell Cycle and Apoptosis Analysis of A549 Cells

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For cell cycle detection, A549 cells were washed with ice-cold PBS three times after being digested by trypsin (Gibco, Waltham, MA, USA). Then, the cells were fixed with 70% ethanol for 8 h and stained with propidium iodide (PI) (Vazyme Biotech Co., Ltd., Nanjing, China) for 10 min in the dark. In cell apoptosis, cells were detached with trypsin-excluded EDTA (Gibco, Waltham, MA, USA) and washed with ice-cold PBS three times. Cells were centrifuged at 300× g at 4 °C for 5 min and then stained with 5 μL of Annexin V-fluorescein isothiocyanate (FITC) and 5 μL of PI (Vazyme Biotech, Nanjing, China, A211-01/02) for 10 min in the dark. Each sample was added to 400 μL of 1 × binding buffer before detection. Cell cycle and cell apoptosis were determined by flow cytometry (BD, Franklin Lakes, NJ, USA).
The cells were collected and then washed with PBS three times. A549 cells were fixed with 4% glutaraldehyde at 4 °C for 72 h, then rinsed again with PBS and refixed with 1% osmium tetroxide for 30 min at room temperature. The following steps proceed based on the procedure described by Winey et al. [27 (link)].

Dai P., Shen J., Shen D., Li X., Win-Shwe T.T, & Li C. (2023). Melatonin Ameliorates Apoptosis of A549 Cells Exposed to Chicken House PM2.5: A Novel Insight in Poultry Production. Toxics, 11(7), 562.

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Polyphyllin I Induces Apoptosis in A375 Cells

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A375 cells were treated with different concentrations of Polyphyllin I for 48 h, and then cells were digested into single cells using EDTA-free trypsin. Lastly, the cells were stained with the Annexin V-FITC and PI solution (Vazyme, Nanjing, China) at 4°C. Cell apoptosis was calculated after 10-15 min by flow cytometry. For detecting cell cycle, each group of A375 cells was harvested by centrifugation, washed, and resuspended with PBS solution. Subsequently, A375 cells were incubated with propidium iodide (PI) (Vazyme, Nanjing, China) for 30 minutes in the dark. At last, cell cycle status was assessed with flow cytometry.

Long J, & Pi X. (2020). Polyphyllin I Promoted Melanoma Cells Autophagy and Apoptosis via PI3K/Akt/mTOR Signaling Pathway. BioMed Research International, 2020, 5149417.

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Apoptosis Evaluation by Flow Cytometry

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Apoptosis was evaluated using flow cytometry with AnnexinV-FITC and propidium iodide (PI) staining (Vazyme Biotech, Nanjing, China). Cells with and without HOXB4 overexpressionwere harvested and washed with ice-cold PBS. The cells were then stained with Annexin V-FITC and PI (BD Biosciences, Franklin Lakes, NY, USA) following the manufacturer's protocol. Flow cytometry utilized an instrument from BD Biosciences.

Zhou G., Liu X., Xiong B, & Sun Y. (2017). Homeobox B4 inhibits breast cancer cell migration by directly binding to StAR-related lipid transfer domain protein 13. Oncology Letters, 14(4), 4625-4632.

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Neutrophil Surface Markers and Apoptosis

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Using the freshly isolated neutrophils, we performed staining to detect neutrophil surface markers and apoptotic cells. The antibody clones (Supplementary Table S1) used for flow cytometric analysis were: Annexin V-APC (Vazyme), propidium iodide (PI; Vazyme), anti-human CD11b-PE (Biolegend), and anti-human CD15-PE-Cy7 (Biolegend). The mixture of these staining antibodies was directly added to suspensions containing 5 × 10⁵ freshly isolated neutrophils and kept for 20 min at room temperature. After this incubation, we washed the cells with PBS and prepared them for analysis.

Wang Q., Ma J., Gong Y., Zhu L., Tang H., Ye X., Su G., Huang F., Tan S., Zuo X., Gao Y, & Yang P. (2024). Sex-specific circulating unconventional neutrophils determine immunological outcome of auto-inflammatory Behçet’s uveitis. Cell Discovery, 10, 47.

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Comprehensive Cell Characterization Assays

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For colony formation assay, stably transfected cells were seeded into six-well plates at a density of 1×10³ cells per well. After 14 days, the colonies were stained with crystal violet staining solution (Beyotime, Shanghai, China). Then, the number of colonies was manually counted. For CCK-8 assay, 100 μL of cell suspension containing 1×10³ cells was added into each well of the 96-well plate. Then, the absorbance at 450 nm was measured at 24, 48, 72 and 96 h after transfection, respectively, using the CCK-8 solution (10 μl) (Dojindo, Kumamoto, Japan). For cell cycle analysis, after adding the propidium iodide (PI) (Vazyme, Nanjing, China), the number of positive cells was analyzed using FACScan. The proportion of cells in the G1, S, and G2/M phases were counted and compared.

Jiang W., Zhang C., Kang Y., Yu X., Pang P., Li G, & Feng Y. (2021). MRPL42 is activated by YY1 to promote lung adenocarcinoma progression. Journal of Cancer, 12(8), 2403-2411.

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Cell Cycle Analysis by Flow Cytometry

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U-251 MG and U-87 MG cells were collected after a 24 h of transfection and then fixed using 70% ethanol in PBS at − 20 °C for 6 h. The cells were then treated with 0.5% Triton X-100 and 10 μg/mL RNase (Sangon Biotech) at 28 °C for 25 min. Finally, the cells were stained with 20 μg/mL propidium iodide (PI, Vazyme) in the dark at 28 °C for 25 min and then placed into to the flow cytometer NovoCyte 1300 (ACEA, San Diego, CA, USA) for fluorescent detection within the PE-channel (Ex: 488 nm/Em: 578 nm).

Chen S., Chen X., Li Z., Mao J., Jiang W., Zhu Z., Li Y., Jiang Z., Zhao W., Tan G, & Wang Z. (2022). Identification of ubiquitin-specific protease 32 as an oncogene in glioblastoma and the underlying mechanisms. Scientific Reports, 12, 6445.

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Cell Viability Analysis of Cryopreserved Cells

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The cell viability of post-cryopreservation also was analyzed by flow cytometry to quantify the cell necrosis and apoptosis. The cells post-cryopreservation were collected and washed in PBS. The cells were then resuspended in binding buffer (0.01 M HEPES pH 7.4, 0.14 M NaCl, and 2.5 mM CaCl₂), stained with 5 μL FITC-conjugated Annexin V and 5 μL propidium iodide (PI) (Vazyme Biotech Co., Ltd., China) in 100 μL binding buffer on ice. The PI and Annexin V were used to stain cells that died of necrosis and apoptosis, respectively. The cells were incubated for 15 min in the dark. The stained samples were analyzed using a flow cytometer (BD FACSCalibur, Becton Dickinson, San Jose, CA, USA) together with the Flowjo software.

Wang J., Zhao G., Zhang Z., Xu X, & He X. (2016). Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification. Acta biomaterialia, 33, 264-274.

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Ethanol-induced Apoptosis in MIN6 Cells

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MIN6 cells were grown in the wells of 6‐well plates and pre‐treated with LNT for 2 hours, and then exposed to ethanol or LNT for an additional 72 hours. The cells were harvested by trypsinization. Following double staining with FITC‐annexin V and propidium iodide (PI) (Vazyme, China), the cells were analysed using flow cytometry.

Wu T., Wang J., Zhang Y., Shao Y., Li X., Guo Y., Dong W., Wang L., Chen F, & Han X. (2021). Lentinan protects against pancreatic β‐cell failure in chronic ethanol consumption‐induced diabetic mice via enhancing β‐cell antioxidant capacity. Journal of Cellular and Molecular Medicine, 25(13), 6161-6173.

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Apoptosis Evaluation via Flow Cytometry

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After incubations, the MEC were washed with PBS three times and harvested by trypsinization, then washed with PBS again, and resuspended in 500 μL 1x binding buffer. The cells were incubated for 10 minutes in the dark at room temperature in the presence of Annexin V-FITC (5 μL) and propidium iodide (PI) (10 μL, Vazyme, China). The cells were immediately analyzed using a flow cytometry.

Xu G., Duan S., Hou J., Wei Z, & Zhao G. (2017). GosB Inhibits Triacylglycerol Synthesis and Promotes Cell Survival in Mouse Mammary Epithelial Cells. BioMed Research International, 2017, 7394869.

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