Hnrnpk

Manufactured by Fortis Life Sciences

HNRNPK is a lab equipment product that serves as a core component in various cellular and molecular biology research applications. It functions as a ribonucleoprotein involved in the regulation of gene expression and RNA processing. The detailed technical specifications and intended use of this product are not available for an unbiased and factual presentation without extrapolation.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Ab177149, by Abcam (1 mentions) G3bp1, by BD (1 mentions) Total eif2α, by Cell Signaling Technology (1 mentions) Pspc1, by Merck Group (1 mentions) Ab32157, by Abcam (1 mentions) Sc 9996, by Santa Cruz Biotechnology (1 mentions) Cpsf6, by Fortis Life Sciences (1 mentions) Pabpc1, by Cell Signaling Technology (1 mentions) Eif4e, by BD (1 mentions) Dazap1, by Fortis Life Sciences (1 mentions) Tdp 43, by Merck Group (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Filaggrin, by Abcam (1 mentions) Keratin 10, by Abcam (1 mentions) Mki67, by Abcam (1 mentions) Alexa 555 conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa 488 conjugated donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Keratin 1, by BioLegend (1 mentions) Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions)

4 protocols using hnrnpk

Comprehensive Antibody Panel for Protein Analysis

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The following commercial primary antibodies were used: G3BP1 (mouse monoclonal; BD Biosciences); TIAR (mouse monoclonal; BD Biosciences); NONO (rabbit polyclonal C-terminal; Sigma-Aldrich); SFPQ (rabbit monoclonal; ab177149, Abcam); CPSF6 (rabbit polyclonal; A301-356A, Bethyl); FUS (mouse monoclonal; 4H11, Santa Cruz); ELAVL1 (rabbit polyclonal, 11910-1-AP, Proteintech); PABPC1 (rabbit polyclonal, Cell Signaling, 4992); EIF4E (mouse monoclonal; BD Biosciences); UBAP2L (rabbit polyclonal; A300-533A, Bethyl); DAZAP1 (rabbit polyclonal; A303-984A, Bethyl); hnRNP K (rabbit polyclonal; A300-674A, Bethyl); PSPC1 (rabbit polyclonal N-terminal; Sigma-Aldrich); TDP-43 (rabbit polyclonal C-terminal; Sigma-Aldrich); HNRNPA3 (rabbit polyclonal; 25142-1-AP, Proteintech); RBM12B (rabbit polyclonal; 17137-1-AP, Proteintech); SRSF9 (rabbit polyclonal; 17926-1-AP, Proteintech); SMARCA5 (rabbit polyclonal; 13066-1-AP, Proteintech); MATR3 (rabbit polyclonal; 12202-2-AP, Proteintech); YBX1 (rabbit polyclonal; 20339-1-AP, Proteintech); GFP (mouse monoclonal; sc-9996, Santa Cruz); Flag (DYKDDDDK tag, mouse monoclonal; 9A3, Cell Signaling); eIF2α phosphorylated at Ser51 (rabbit monoclonal; ab32157, Abcam); total eIF2α (rabbit monoclonal; D7D3, Cell Signaling); and β-actin (mouse monoclonal; A5441, Sigma-Aldrich). Antibodies were used at 1:1,000 dilution for all applications unless stated otherwise.

An H., Tan J.T, & Shelkovnikova T.A. (2019). Stress granules regulate stress-induced paraspeckle assembly. The Journal of Cell Biology, 218(12), 4127-4140.

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Multiplex Immunofluorescence of Epidermal Markers

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Cultured cells or tissue were fixed in 4% paraformaldehyde for 11 min followed by blocking in PBS with 2.5% normal goat serum, 0.3% triton X-100, and 2% bovine serum albumin for 30 min Primary antibodies used were Keratin 1 (Biolegend: PRB-149P) at 1:1000, Filaggrin (Abcam: ab3137) at 1:200, MKi67 (Abcam: ab16667) at 1:300, Keratin 10 (Abcam: ab9025) at 1:500, HNRNPK (Bethyl Laboratories: A300–674A) at 1:1000 for 1 h. The secondary antibodies used were Alexa 555 conjugated goat anti-mouse IgG (ThermoFisher: A11029) or Alexa 488 conjugated donkey anti-rabbit IgG (ThermoFisher: A21206) both at 1:500. Nuclear dye, Hoechst 33342 was used at 1:1000 (ThermoFisher: H3570).

Li J., Chen Y., Xu X., Jones J., Tiwari M., Ling J., Wang Y., Harismendy O, & Sen G.L. (2019). HNRNPK maintains epidermal progenitor function through transcription of proliferation genes and degrading differentiation promoting mRNAs. Nature Communications, 10, 4198.

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Immunoblotting for RNA-binding Proteins

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Twenty microgram of the cell lysates were used for immunoblotting and resolved on 10% SDS-PAGE and transferred to PVDF membranes. Primary antibodies used include beta-actin (Santa Cruz: sc-47778) at 1:5000, HNRNPK (Bethyl Laboratories: A300–674A) at 1:3000, DDX6 (Novus Biologicals: NB200–192) at 1:2000, RNA Pol (Active motif: 39097) at 1:4000, Cyclin T1 (Bethyl Laboratories: A303–499A) at 1:1000, XRN2 (Cell Signaling: 13760) at 1:1000 and CDK9 (Bethyl Laboratories: A303–493A) at 1:1000. Secondary antibodies including donkey anti-rabbit IgG HRP (Sigma: NA934V) and goat anti-mouse IgG-HRP (Santa Cruz: sc-2005) were used at 1:2000. All original blots can be found in the accompanying Source Data.

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Chromatin Immunoprecipitation and Microscopy Techniques

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The following primary antibodies were used for ChIP-seq: H3K27me3 (GeneTex, GTX60892), H2AK119ub (Cell Signaling, CST8240); for IF: H3K27me3 (GeneTex, GTX60892), H3K27me3 (Active Motif, AM39535), H2AK119ub (Cell Signaling, CST8240), EZH2 (Cell Signaling, CST3147), HNRNPK (Proteintech, 11426-1-AP), HNRNPK (Bethyl Laboratories, A300-674A), HNRNPE1 (Proteintech, 14523-1-AP), HNRNPE2 (GeneTex, GTX114616), HNRNPE3 (GeneTex, GTX118656), CIZ1 (in-house (Sunwoo et al., 2017 )); for Western blot: H3K27me3 (GeneTex, GTX60892), H3K27me3 (Cell Signaling, CST9733), H2AK119ub (Cell Signaling, CST8240), GAPDH (Cell Signaling, CST14C10), HNRNPK (Proteintech, 11426-1-AP). Dye-conjugated secondary antibodies were purchased from Life Technologies.

Colognori D., Sunwoo H., Kriz A., Wang C.Y, & Lee J.T. (2019). Xist deletional analysis reveals a co-dependency between Xist RNA and Polycomb complexes for spreading along the inactive X. Molecular cell, 74(1), 101-117.e10.

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