B6.129p2 nos2tm1lau j nos2

Mice were bred and maintained under specific pathogen-free conditions. Female, 10–12-week-old C57BL/6 (B6), B6.129S2-Ighm^tm1Cgn/J (μMT), B6.129P2-Il10^tm1Cgn/J (IL-10−/−), B6.129X1-Elane^tm1Sds/J (Elastase−/−), and B6.129S4-C3^tm1Crr/J (C3−/−), B6.129S7-Itgb2 ^tm1Bay/J (CD18−/−), B6(Cg)-Ifnar1^tm1.2Ees/J (IFNAR−/−), B6N.129S2-Casp1^tm1Flv/J (Casp1−/−), and B6.129P2-Nos2^tm1Lau/J (Nos2−/−) mice were from Jackson Laboratory (Bar Harbor, ME). C57BL/6J-Ticam1 ^Lps2/J (Trif ^Lps2; TRIF−/−) and B6.129P2(SJL)-MyD88^tm1.1Defr/J (MyD88−/−) mice were bred at the University of Florida. To induce lupus, 0.5 mL of pristane (Sigma-Aldrich, St. Louis, MO) was administered i.p. Controls were left untreated. Peritoneal exudate cells were collected by lavage. In some experiments, bronchoalveolar lavage (BAL) was performed. After euthanizing the mice, a small incision was cut in the trachea and the alveolar spaces were lavaged with 1 ml of PBS. Cells collected by BAL were resuspended in RPMI1640 + 10% fetal bovine serum and incubated at 37° C for 1-hr before treating with IL-10 (1 ng/ml). After 15-min incubation, the cells were fixed, permeabilized, and surface-stained with anti-CD11b antibodies and intracellularly-stained with anti-phospho-Stat3 antibodies as below. These studies were approved by the Institutional Animal Care and Use Committee.

Irg1^−/− C57BL/6N mice were described previously (Lampropoulou et al., 2016 (link)). C57BL/6N used as WT controls in experiments with Irg1^−/− mice were obtained from Charles River Laboratories. B6.129X1-Nfe2l2^tm1Ywk/J (Nrf2^−/−), C57BL/6N-Gsdmd^em4Fcw/J (Gsdmd^−/−), B6.Cg-Gt(ROSA)26Sor^{tm1(CAG-Pycard/mCitrine*,-CD2*)Dtg}/J (ASC-mCitrine (Tzeng et al., 2016 (link)), B6.129P2-Nos2^tm1Lau/J (Nos2^−/−), B6(Cg)-Ifnar1^tm.2Ees/J (Ifnar1^−/−) and WT C57BL/6J mouse strains were purchased from Jackson Laboratory. ROSA26-CreER^T2/SDHB^{floxed/floxed} (Sdhb^fl/fl) and ROSA26-CreER^T2/SDHB^{wild-type/wild-type} mice (Sdhb WT) as previously published (Cardaci et al., 2015 (link)) were provided by Eyal Gottlieb (University of Glasgow, UK). Mice were maintained at Washington University under specific pathogen-free conditions in accordance with Federal and University guidelines and protocols approved by the Animal Studies Committee of Washington University. Femurs and tibias from Atf3^−/− mice as described (Jiang et al., 2004 (link)) were provided by Tsonwin Hai (Ohio State University). Mice used for in vitro experiments were 8- to 24-week-old, age and sex-matched and both sexes were used. For in vivo experiments 9 to 14-week-old female mice were used in the model of endotoxin-induced sepsis and 10 to 12-week-old male mice were used in the psoriasis model.

C57BL/6 (WT) male mice were purchased from Jackson Laboratory or Taconic. B6.129S2-Il6^tm1Kopf/J (Il6^−/−) and B6.129P2-Nos2^tm1Lau/J (Nos2^−/−) male mice were purchased from Jackson Laboratory. B6.129S2-Nos2^tm1MrI N12 (Nos2^−/−) male mice were purchased from Taconic. P14 TCR transgenic mice were previously described⁶⁰ . C57BL/6-Tg(TcraTcrb)1100Mjb/J (OT-1) male mice were purchased from Jackson Laboratory. All male mice were used at 5–12 weeks of age. For infectious experiments, mice were housed in a specific pathogen-free barrier facility and used in accordance with animal care guidelines from the Harvard Medical Area Standing Committee on Animals and the National Institutes of Health. All other animals were maintained in specific pathogen-free facilities at the Dana-Farber Cancer Institute and were approved by the Animal Care and Use Committee of the Dana-Farber Cancer Institute.