Fractionation of Mgryph cells, SDS-PAGE and western blotting were performed as described previously20 (link). All samples were loaded on the same polyacrylamide gel and blotted on a PVDF membrane. Immunodetection was performed by chemiluminescence, using an anti-MamC44 (link) and an anti-MCherry primary antibody simultaneously and a horseradish peroxidase conjugated anti-rabbit IgG secondary antibody. The luminescence signal was generated with Western Blot Quant substrate (Takara) and detected using a ChemiDoc™ XRS+ system with ImageLab software (Biorad). Densiometric analyses were performed with ImageLab software only on images without saturated pixels.
Western blot quant substrate
Manufactured by Takara Bio
The Western Blot Quant substrate is a chemiluminescent substrate designed for the quantitative detection of proteins on Western blots. It is used in conjunction with Western blotting techniques to provide a sensitive and accurate method for measuring the abundance of target proteins in a sample.
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Lab products found in correlation
2 protocols using western blot quant substrate
1
MagGryphon Cell Fractionation and Western Blot
2
MagGryphon Cell Fractionation and Western Blot
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Fractionation of Mgryph cells, SDS-PAGE and western blotting were performed as described previously20 (link). All samples were loaded on the same polyacrylamide gel and blotted on a PVDF membrane. Immunodetection was performed by chemiluminescence, using an anti-MamC44 (link) and an anti-MCherry primary antibody simultaneously and a horseradish peroxidase conjugated anti-rabbit IgG secondary antibody. The luminescence signal was generated with Western Blot Quant substrate (Takara) and detected using a ChemiDoc™ XRS+ system with ImageLab software (Biorad). Densiometric analyses were performed with ImageLab software only on images without saturated pixels.
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