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Dm6000 cs

Manufactured by Leica
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The Leica DM6000 CS is a compact, high-performance compound microscope designed for routine laboratory use. It features a robust, ergonomic design and offers a range of objective lenses and illumination options to support various applications. The DM6000 CS provides clear, high-resolution imaging for examination and analysis tasks.

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Lab products found in correlation

Hoechst, by Thermo Fisher Scientific (2 mentions) Paraformaldehyde (pfa), by Merck Group (2 mentions) Hcx irapo l 25x 0.95 w objective, by Leica (1 mentions) Las af software, by Leica (1 mentions) Prism 9, by GraphPad (1 mentions) H 3300, by Vector Laboratories (1 mentions) Dfc360 fx, by Leica (1 mentions) Las af 3, by Leica (1 mentions) Stemi 2000 c dissecting microscope, by Zeiss (1 mentions) Dm lb2, by Leica (1 mentions) Tcs sp8, by Leica (1 mentions) Image pro plus 6, by Media Cybernetics (1 mentions) Snap cell oregon green, by New England Biolabs (1 mentions) Snap surface 549, by New England Biolabs (1 mentions) Las af, by Leica (1 mentions)

Close spelling variants of this product

DM6000 CS confocal microscope TCS SP5 (DM6000 CS) DM6000

13 protocols using dm6000 cs

1

Confocal Imaging of Bacterial Biofilms

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Biofilms were grown overnight in TSB in flat 8-chamber plate Nunc Lab-TekTM Chamber Slides (ThermoFisher Scientific) for 24 h at 37 °C. After that, the medium was removed, and the biofilms were washed twice with sterile PBS to remove non-adherent cells. Bacterial inside biofilms were stained with LIVE/DEAD BacLight Bacterial Viability Kit staining, according to the manufacturer´s instructions. Briefly, biofilms were incubated 15 min in dark, after that these were washed twice with sterile PBS to remove the excess colorants. The controls biofilms were approximately 30 to 50 µm thick, and Z-slices were obtained every 0.5 microns. All confocal images and stacks of images were obtained at the Microscopy Center of Universidad Nacional de Córdoba. Each individual chamber was analyzed in a confocal laser scanning microscope (CLSM) Leica DM6000 CS (Leica Microsystems, Wetzlar, Germany), using a 60x oil immersion upright objective. The images and stacks of images were analyzed using the FIJI-IMAGEJ analysis software.
Breser M.L., Felipe V., Bohl L.P., Orellano M.S., Isaac P., Conesa A., Rivero V.E., Correa S.G., Bianco I.D, & Porporatto C. (2018). Chitosan and cloxacillin combination improve antibiotic efficacy against different lifestyle of coagulase-negative Staphylococcus isolates from chronic bovine mastitis. Scientific Reports, 8, 5081.
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2

Quantifying YFP Fluorescence in SGLT Oocytes

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YFP fluorescence intensity of oocytes expressing hSGLT3, hSGLT1 and mutants thereof was measured with a confocal laser scanning microscopy (Leica DM6000 CS, Leica Microsystems CMS GmbH, Wetzlar, Germany) equipped with a Leica HCX IRAPO L25x/095W objective. The optical plane was set to the equator of the oocyte and the settings for YFP fluorescence acquisition and laser intensity were kept constant for all tested oocytes. YFP-fluorescence intensity of a quarter of the oocyte was quantified using the LAS AF software (Leica Microsystems CMS GmbH, Wetzlar, Germany).
Schäfer N., Friedrich M., Jørgensen M.E., Kollert S., Koepsell H., Wischmeyer E., Lesch K.P., Geiger D, & Döring F. (2018). Functional analysis of a triplet deletion in the gene encoding the sodium glucose transporter 3, a potential risk factor for ADHD. PLoS ONE, 13(10), e0205109.
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3

Oospore Characterization of Chara Species

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Oospores of S276, NIES-1604, LaT-2708 and Ranstadt were analyzed by quantitative and qualitative morphology parameters, defined after Soulié-Märsche and García, 2014 (link). Therefore, oospores were placed on microscope slides with an adhesive surface and documented with a stereomicroscope in lateral, apical and basal view (Leica DM6000 CS). Oospore colour, shape and appendixes such as claws or cages were determined with LI to avoid misinterpretation by photodocumentation. NIES-1604 oospores were pre-treated with sonication for 2minutes at 20°C and 10% Power (Sonorex Super 10P). Parameters, such as number of striae, length, width, fossa width, basal impressions and angle of stria to the longitudinal axis were measured with ImageJ. Statistical data analyses for differences between strains were performed using GraphPad Prism 9.3.1, see paragraph 2.3. (Graph Pad Software, San Diego, USA).
Holzhausen A., Stingl N., Rieth S., Kühn C., Schubert H, & Rensing S.A. (2022). Establishment and optimization of a new model organism to study early land plant evolution: Germination, cultivation and oospore variation of Chara braunii Gmelin, 1826. Frontiers in Plant Science, 13, 987741.
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4

Quantification of Phospho-Tau Puncta

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Sections were rehydrated to water as follows: 100% EtOH for 3 min, 100% EtOH for 3 min, 95% EtOH for 1 min, 80% EtOH for 1 min, H2O for 5 min. Antigen retrieval was performed by boiling slides in citric acid buffer (Vector; H-3300) at 95°C for 40 min. Sections were then permeabilized in PBS with 0.1% Tween-20 (PBSTw), blocked in 3% bovine serum albumin (BSA) for 1 h at RT then incubated in 1° antibody overnight at 4°C. Sections were washed (2x, PBSTw) then incubated in 2° antibody for 1 h at RT. See Supplementary Table 4 for antibody information. Sections were washed in PBSTw, rinsed in deionized water then coverslipped with mounting media. For phospho-tau puncta quantification, sections were imaged on a Leica DFC360 FX under a 10x objective, 1.2x magnification and identical exposure settings. Puncta were counted in FIJI using the cell counter. Puncta were counted in one brain hemisphere across 5 non-consecutive sections (every 3rd section) across the same brain level for all samples to calculate puncta per 1002μM2. High resolution representative images were acquired using a 20x and 60x objective (oil immersion) on a Leica DM6000 CS. Image acquisition and analysis was performed blind to sample identity.
Byrns C.N., Saikumar J, & Bonini N.M. (2021). Glial AP1 is activated with aging and accelerated by traumatic brain injury. Nature aging, 1(7), 585-597.
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5

Immunofluorescence Staining of HEK293T Cells

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HEK293T cells were grown on poly‐L‐lysine‐coated glass coverslips. Cells were transfected for 24 h and fixed in 4% paraformaldehyde (Sigma) for 30 min at RT. The fixed cells were washed twice with cold PBS, permeabilized, and blocked with 0.1% Triton X‐100/5% BSA/PBS for 30 min. The permeabilized cells were incubated overnight at 4 °C in the dark with primary antibody (1:200 dilution) and then washed by PBS 5 times, followed by incubation with secondary antibody at RT in the dark for 1 h (1:1000) and 5 times wash by PBS. Hoechst (Thermo) was next used to stain nuclei for 10 min, followed by 3 times washing by PBS. Samples were observed using inverted confocal microscopy (DM6000CS, Leica).
Zheng S., Lin J., Pang Z., Zhang H., Wang Y., Ma L., Zhang H., Zhang X., Chen M., Zhang X., Zhao C., Qi J., Cao L., Wang M., He X, & Sheng R. (2022). Aberrant Cholesterol Metabolism and Wnt/β‐Catenin Signaling Coalesce via Frizzled5 in Supporting Cancer Growth. Advanced Science, 9(28), 2200750.
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6

Fetal Testis Imaging and Analysis

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Fetuses from timed matings were dissected at E16.5 and E18.5 to collect testes. After excision, testes were washed in PBS followed by mounting on a slide (Thermo Fisher Scientific, cat# 12-550-15) in polyvinyl alcohol (PVA) with a coverslip (VWR, cat# 48366–227). Mounted samples were directly imaged using a SP8 Leica DM6000CS upright confocal microscope with affiliated Leica software (LAS AF 3). All tile stitching was done with Leica software, remaining image processing was done with FIJI (version 2.3.0/1.53f) [75 (link)] and Adobe Photoshop (version 23.1.0).
Ruthig V.A., Hatkevich T., Hardy J., Friedersdorf M.B., Mayère C., Nef S., Keene J.D, & Capel B. (2023). The RNA binding protein DND1 is elevated in a subpopulation of pro-spermatogonia and targets chromatin modifiers and translational machinery during late gestation. PLOS Genetics, 19(3), e1010656.
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7

Zebrafish Tumor Xenograft Model for Anti-Cancer Evaluation

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Our previous work showed that implanting U87 tumor cells into the perivitelline space of zebrafish allowed for tumor growth in zebrafish embryos43 (link). Therefore, these zebrafish with tumor xenografts could serve as an ideal model to investigate the anti-tumor effect of our nano-micelles. Indeed, the U87 tumor cells were also transfected with EGFP by lentivirus in order to aid in analysis. Zebrafish embryos were stripped off the egg sheath and anesthetized with 0.01% tricaine at 48 hpf. Using a Zeiss Stemi 2000-C dissecting microscope (Carl Zeiss Microimaging Inc., Thornwood, NY), zebrafish were then injected with 300 tumor cells into the perivitelline space using a Cell Tram Vario injector (Eppendorf, USA) with a glass micropipette (50 mm length, diameter of the needle opening about 25 μg/mL). At 24 h post-implantation, Holtfreter’s solution (control), blank micelles, H-M nano-micelles, DOX-M nano-micelles, and DOX-H-M nano-micelles were added into the incubating Holtfreter’s solution at a final DOX and/or HK concentration of 1 μg/mL. Finally, images of the tumors were taken using a confocal microscope (DM6000 CS, Leica, Germany) at 5 days post-implantation.
Gao X., Yu T., Xu G., Guo G., Liu X., Hu X., Wang X., Liu Y., Mao Q., You C, & Zhou L. (2017). Enhancing the anti-glioma therapy of doxorubicin by honokiol with biodegradable self-assembling micelles through multiple evaluations. Scientific Reports, 7, 43501.
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8

Visualizing Mauthner Cell Dendritic Structure

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We dissected the brains of fixed larvae using custom-made tools made of sharpened tungsten wires attached to glass pipettes. We moved each brain to a drop of glycerol in a custom-made coverslip chamber. Briefly, we attached two 20×20 mm #1.5 coverslips to a 20×40 mm #1 coverslip with a room-temperature-hardening mixture made of equal weights of petroleum-jelly, lanolin, and paraffin (VALAP ), creating a vertical channel. We placed fixed brains in the channel, covered it with another 20×20 #1.5 coverslip and used VALAP to seal the channel. To image whole brains we used a laser scanning confocal microscope (Leica SP8; microscope: DM6000CS; objectives: Leica 20x/NA0.70 HC PL APO, Leica 40x/NA1.30 HC PL APO oil or Leica 63x/NA1.40 HC PL APO oil, with lateral resolutions of 414 nm, 223 nm, and 207 nm respectively; laser lines: 561 nm (for Dextran-TexasRed) and 488 nm (for Dextran-Alexa488)). We collected multiple optical slices (thickness optimized by the confocal software, ranging 0.34- 0.35 μm for the 63X objective, and 0.41- 0.42 μm for the 40X objective, and 0.95 μm for the 20X objective) that contained the complete dendritic structure of Mauthner cells in each brain.
Tanvir Z., Rivera D., Severi K.E., Haspel G, & Soares D. (2020). Evolutionary and homeostatic changes in morphology of visual dendrites of Mauthner cells in Astyanax blind cavefish. The Journal of comparative neurology, 529(8), 1779-1786.
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9

Bimolecular Fluorescence Complementation Assay

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To construct vectors for BiFC analysis, the open reading frames (ORFs) of SGT1 and SRC2-1 (flanked by the attB sequence) were amplified with gene-specific primer pairs listed in Supplementary Table S2. Products were introduced into pDONR207 to yield pDONR vectors (named SGT1-207 and SRC2-1-207), and then transferred into the satellite vectors pE3130 and pE3136 to generate SGT1-YFPN and SRC2-1-YFPC. The reciprocal constructs, SGT1-YFPC and SRC2-1-YFPN, were constructed similarly. Fragments of SGT1-YFPN and SRC2-1-YFPC or SGT1-YFPC and SRC2-1-YFPN in satellite vectors were together recombined into the destination vector PE3519, which was then transformed into the Agrobacterium strain GV3101. Agrobacterial cells harboring the BiFC constructs were Agro-infiltrated into the leaves of N. benthamiana plants. Two days after Agro-infiltration, fluorescent signals from N. benthamiana leaves were analyzed using a confocal microscope DM6000 CS (Leica, Solms, Germany). For DAPI staining, N. benthamiana leaves were immersed in DAPI solution (0.1% DAPI and 5% DMSO) and incubated at 37 °C for 1 h before observation.
Liu Z.Q., Liu Y.Y., Shi L.P., Yang S., Shen L., Yu H.X., Wang R.Z., Wen J.Y., Tang Q., Hussain A., Khan M.I., Hu J., Liu C.L., Zhang Y.W., Cheng W, & He S.L. (2016). SGT1 is required in PcINF1/SRC2-1 induced pepper defense response by interacting with SRC2-1. Scientific Reports, 6, 21651.
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10

Immunofluorescence Assay for LC3B

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The cells were fixed in 4% paraformaldehyde (PFA), permeabilized with 0.2% Triton X-100, blocked with 1% bovine serum albumin (BSA) at room temperature, and then incubated with anti-LC3B primary antibody (1:100 dilution) at 4 °C overnight. The FITC goat anti-rabbit IgG (H + L) secondary antibody (Apexbio) was incubated at room temperature for 2 h in the dark. The nuclei were stained with Hoechst 33342 for 5 min. An inverted confocal microscopy (DM6000CS, Leica, Wetzlar, Germany) was used to capture the images.
Jiang X., Li G., Zhu B., Yang J., Cui S., Jiang R, & Wang B. (2024). p20BAP31 Induces Autophagy in Colorectal Cancer Cells by Promoting PERK-Mediated ER Stress. International Journal of Molecular Sciences, 25(10), 5101.
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