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A054 1

Manufactured by Nanjing Jiancheng
Sourced in China

The A054-1 is a laboratory equipment designed for scientific research and analysis. It serves as a tool for performing various experiments and tests in a controlled environment. The core function of this product is to facilitate precise measurement and data collection, enabling researchers to gather accurate and reliable information. The detailed specifications and intended use of this equipment are not available in an unbiased and factual manner without further interpretation or extrapolation.

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3 protocols using a054 1

1

Characterization of Plasma Immunoglobulins and Digestive Enzymes

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The plasma samples were prepared by centrifuging the collected blood samples at 1000 × g at 4°C for 10 min (Sun et al., 2016 ). The concentrations of immunoglobulin (Ig) A, IgG, and IgM and endotoxin in plasma were measured with ELISA kits (A40199-S, A04022-S, A02721-S, A049165-S; Shanghai Jining Shiye Co. Ltd., Shanghai, China), according to the manufacturer's instructions. The activities of α-amylase, lipase, and chymotrypsin in chyme from the duodenum and jejunum were determined by a colorimetric method using specific assay kits (C016-1, A054-1, A080-1; Nanjing Jiancheng Bioengineering Institute of China, Nanjing, China).
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2

Antioxidant and Intestinal Function Analysis

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Total antioxidant capacity (T-AOC), glutathione peroxidase (GPX), catalase (CAT) and superoxide dismutase (SOD) activities and malondialdehyde (MDA) and protein carbonyl (PC) concentrations were determined by their specific assay kits (A015-1-2, A005-1-2, A007-1-1, A001-3-2, A003-1-2, and A087-1-2) from the Nanjing Jiancheng Bioengineering Institute of China. Protein concentration was analyzed by the bicinchoninic acid assay [19 (link)]. Jejunal chyme lipase, α-amylase, and neutral protease activities were measured by their specific assay kits (A054-1, C016-1, A080-3-1; Nanjing Jiancheng Bioengineering Institute, Nanjing, China) [20 (link)]. Small intestinal histopathology and morphology were analyzed as previously described [18 (link)]. Briefly, the duodenum, jejunum, and ileum tissues were washed with saline, fixed in 10% neutral buffered formalin, embedded in paraffin, sectioned at 5 μm, and then stained with hematoxylin and eosin. All slides were examined by a microscope.
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3

Serum Amylase and Lipase Assay

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Serum amylase and lipase were measured with assay kits (C016‐1 and A054‐1) from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
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