Boric acid was acquired from Chem-Lab (Zedelgem, Belgium). HPLC-grade methanol and acetonitrile and sodium azide (99%) were from Honeywell Riedel-de Haën (Seelze, Germany). Hydrochloric acid ≥ 37% and sulfuric acid 96–97% were obtained from Honeywell Fluka (Düsseldorf, Germany). Disodium tetraborate decahydrate (99–103%), disodium hydrogen phosphate anhydrous (≥99%), potassium hydroxide, trichloroacetic acid (≥98%), and Kjeldahl catalyst tablets were from Merck (Darmstadt, Germany). Sodium hydroxide was from LabChem (Loures, Portugal). Borate buffer, o-phthalaldehyde/3-mercaptopropionic acid (OPA/3-MPA), and 9-fluorenylmethyl chloroformate (FMOC) were from Agilent Technologies (Palo Alto, CA, USA). The amino acid kit containing the individual standards (≥99%) of L-alanine, L-arginine hydrochloride, L-asparagine, L-aspartic acid, L-cysteine, L-cystine, L-glutamic acid, L-glutamine, glycine, L-histidine hydrochloride, trans-4-hydroxy-L-proline, L-isoleucine, L-leucine, L-lysine hydrochloride, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine, and L-valine and L-norvaline was from Sigma-Aldrich (Darmstadt, Germany). Ultrapure water was obtained from a Seralpur PRO 60 CN and Seradest LFM 20 water purification system (Ransbach-Baumbach, Germany).
9 fluorenylmethyl chloroformate fmoc
Manufactured by Agilent Technologies
Sourced in United States
9-fluorenylmethyl chloroformate (FMOC) is a chemical reagent used in peptide synthesis and amino acid analysis. It is a protecting group that can be used to temporarily block the amino group of amino acids during peptide synthesis. FMOC is a useful tool for controlling the step-by-step construction of peptide molecules.
Automatically generated - may contain errors
Lab products found in correlation
Borate buffer, by Agilent Technologies (2 mentions)
O phthalaldehyde 3 mercaptopropionic acid opa 3 mpa, by Agilent Technologies (2 mentions)
L norvaline, by Merck Group (1 mentions)
L tryptophan, by Merck Group (1 mentions)
L phenylalanine, by Merck Group (1 mentions)
Di sodium tetraborate decahydrate, by Merck Group (1 mentions)
Kjeldahl catalyst tablet, by Merck Group (1 mentions)
L lysine hydrochloride, by Merck Group (1 mentions)
L cystine, by Merck Group (1 mentions)
L asparagine, by Merck Group (1 mentions)
L methionine, by Merck Group (1 mentions)
L threonine, by Merck Group (1 mentions)
L valine, by Merck Group (1 mentions)
L isoleucine, by Merck Group (1 mentions)
L serine, by Merck Group (1 mentions)
L alanine, by Merck Group (1 mentions)
L tyrosine, by Merck Group (1 mentions)
Trans 4 hydroxy l proline, by Merck Group (1 mentions)
L proline, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
3 protocols using 9 fluorenylmethyl chloroformate fmoc
1
Analytical Methods for Amino Acid Quantification
2
HPLC Amino Acid Quantification
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Amino acids standards (> 99% purity) (Sigma Aldrich®, Saint-Louis, Missouri, USA) were used for identification and quantification by HPLC. Chemicals for buffer solution were all of analytical grade: disodium hydrogen phosphate (Na2HPO4 anhydrous), sodium tetraborate decahydrate (Na2B4O7; 10H2O), sodium azide (NaN3), hydrochloric acid 37% (HCl), and phosphoric acid 85% (H3PO4) (Thermo Fisher Scientific®, Waltham, Massachusetts, USA). The reagents for derivatization of amino acids (3-mercaptopropionic acids (3-MPA), ortho-phthalaldehyde (OPA), and 9-fluorenylmethylchloroformate (FMOC)), and borate buffer (0.4 M in water, pH 10.2) were obtained from Agilent® technologies (Santa Clara, California, USA) and were stored at 4 °C. Acetonitrile (ACN) and methanol (MeOH) were of HPLC grade and received from Thermo Fisher Scientific® (Waltham, Massachusetts, USA). Water was purified through a Purelab® Option Q7 purifying system (Veolia® water technologies, Saint-Maurice, France). The buffer solution was filtered through polyvinylidene fluoride (PVDF) at 0.22 µm (Thermo Fisher Scientific®, Waltham, Massachusets, USA) and all samples were filtered through regenerated cellulose (RC) at 0.20 µm (Thermo Fisher Scientific®, Waltham, Massachusetts, USA).
3
Enzymatic Hydrolysis of Proteins
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Flavourzyme®, a protease and exopeptidase preparation from Aspergillus oryzae, was supplied by Sigma (Dublin, Ireland) and Prolyve® 1000, a protease from Bacillus licheniformis, was kindly provided by Lyven Enzymes Industrielles (Caen, France). HPLC-grade water and acetonitrile were from VWR International (Dublin, Ireland). Trinitrobenzene sulphonic (TNBS) acid solution was from Fisher Scientific (Dublin, Ireland). 2,2′-Azinobis-(3-ethylbenzothiazoline-6-sulfonate (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), fluorescein, 2,2′-azobis-2-methyl-propanimidamide (AAPH), Trolox, amino acid standards, dihydrorhodamine 123 (DHR) and sodium hypochlorite solution (with 4% available chlorine) were obtained from Sigma (St. Louis, MO, USA). o-Phthalaldehyde/3-mercaptopropionic acid (OPA/3-MPA) and 9-fluorenylmethyl chloroformate (FMOC) were from Agilent Technologies (Palo Alto, CA, USA).
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