Cisplatin

Manufactured by Adooq

Sourced in United States

Cisplatin is a platinum-based compound used as a laboratory reagent. It is a crystalline solid that is soluble in water and dimethyl sulfoxide. Cisplatin is commonly used in research applications involving cell culture and molecular biology.

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Lab products found in correlation

Olaparib, by Apexbio (1 mentions) Veliparib abt 888, by Selleck Chemicals (1 mentions) Niraparib, by Selleck Chemicals (1 mentions) Rucaparib, by Selleck Chemicals (1 mentions) Cisplatin, by Apexbio (1 mentions) Carboplatin, by Apexbio (1 mentions) 5 fluorouracil, by Apexbio (1 mentions) Nms p118, by Selleck Chemicals (1 mentions) Art558, by MedChemExpress (1 mentions) Nedisertib, by MedChemExpress (1 mentions) Azd7648, by MedChemExpress (1 mentions) Rad51 inhibitor b02, by MedChemExpress (1 mentions) Olaparib, by MedChemExpress (1 mentions) Snu1196, by Korean Cell Line Bank (1 mentions) Snu 308, by Korean Cell Line Bank (1 mentions) Tfk 1, by RIKEN Cell Bank (1 mentions) Tgbc 24tkb, by RIKEN Cell Bank (1 mentions) Hucct1, by RIKEN Cell Bank (1 mentions) Ssp 25, by RIKEN Cell Bank (1 mentions) Recombinant human il 6, by Thermo Fisher Scientific (1 mentions)

4 protocols using cisplatin

Comprehensive Synthetic Compound Library

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Olaparib (Catalog# A4154), cisplatin (Catalog# A8321), carboplatin (Catalog# A2171), and 5-fluorouracil (Catalog# A4071) were all purchased from APExBIO company. UPF 1096 (Catalog# S8038), NMS-P118(Catalog# S8363), stenoparib (E7449) (Catalog# S8419), niraparib (Catalog# S2741), rucaparib (Catalog# S4948), and veliparib (ABT-888) (Catalog# S1004) were all purchased from Selleckchem.
cisplatin (Catalog# A10221) was purchased from AdooQ Bioscience. ART-558(Catalog# HY-141520), DNA-PK inhibitors PIK-75 hydrochloride (Catalog# HY-13281), Nedisertib (Catalog# HY-101570) and AZD-7648 (Catalog# HY-111783), RAD51 Inhibitor B02 (Catalog# HY-101462), and Olaparib (for in vivo experiment: Catalog# HY-10162) were all purchased from MCE (MedChem Express). ART-812 was synthetized by Dr. Wayne Childers at Temple University School of Pharmacy. All compounds were dissolved, aliquoted, and stored following the manufacturer’s instructions.

Li H., Chatla S., Liu X., Vekariya U., Kim D., Walt M., Lian Z., Morton G., Feng Z., Yang D., Liu H., Reed K., Childers W., Yu X., Madzo J., Chitrala K.N., Skorski T, & Huang J. (2023). Haploinsufficiency of ZNF251 causes DNA-PKcs-dependent resistance to PARP inhibitors in BRCA1-mutated cancer cells. Research Square.

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Establishment of Gemcitabine-Resistant BTC Cell Lines

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BTC cell lines (RBE, HuCCT1, SSP-25, TGBC-24TKB, and TFK-1) were purchased from the RIKEN Cell Bank (Ibaraki, Japan). BTC cell lines (SNU-308 and SNU-1196) were purchased from the Korean cell line bank (Seoul, Korea). RBE, HuCCT1, SSP-25, TFK-1 SNU-308, and SNU-1196 cell lines were grown in RPMI (Roswell Park Memorial Institute) medium supplemented with 10% fetal bovine serum (FBS), penicillin-streptomycin, L-glutamine, and sodium pyruvate. TGBC-24TKB cells were grown in DMEM supplemented with 10% FBS and penicillin–streptomycin. The gemcitabine-resistant cells (SNU-1196-GR and SSP-25-GR cells) were generated in the cell culture system. The cells were treated with a half-maximal inhibitory concentration (IC₅₀) dose of gemcitabine for one month (20 nM for SSP-25 cells and 10 nM for SNU-1196 cells). After one month, the media were replaced with the fresh media containing an IC₉₀ dose of gemcitabine (250 nM for SSP-25 cells and 60 nM for SNU-1196 cells) for another month. After two months, the live cells were identified as gemcitabine-resistant cells. All the cell lines used in this study were tested for mycoplasma contamination and authenticated by STR (short tandem repeat) method. AZD0156 (ATM inhibitor), gemcitabine, and cisplatin were purchased from AdooQ BioScience (Irvine, CA, USA).

Pan Y.R., Wu C.E, & Yeh C.N. (2020). ATM Inhibitor Suppresses Gemcitabine-Resistant BTC Growth in a Polymerase θ Deficiency-Dependent Manner. Biomolecules, 10(11), 1529.

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Chemical Synthesis of Hispolon Methyl Ether

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Hispolon methyl ether was chemically synthesized according to the published procedure [27 (link)]. Hispolon was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Cisplatin was obtained from AdooQ^® Bioscience (Irvine, CA, USA). Recombinant human IL-6 was acquired from PeproTech (Rehovot, ISR).

Kuo M.Y., Yang W.T., Ho Y.J., Chang G.M., Chang H.H., Hsu C.Y., Chang C.C, & Chen Y.H. (2022). Hispolon Methyl Ether, a Hispolon Analog, Suppresses the SRC/STAT3/Survivin Signaling Axis to Induce Cytotoxicity in Human Urinary Bladder Transitional Carcinoma Cell Lines. International Journal of Molecular Sciences, 24(1), 138.

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Cytotoxicity of Carfilzomib and Cisplatin

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Carfi lzomib and cisplatin were purchased from AdooQ Bio-Science (CA, USA) and dissolved in dimethyl sulfoxide (DMSO) (Pars Tous Biotechnology, Mashhad, Iran) and stored at -20 °C until further use. cisplatin was purchased from Sigma-Aldrich (St. Louis, MO, USA) and freshly solubilized in predetermined amounts of phosphate buffer with a relatively acidic pH, and kept away from light. RNA extraction kit was obtained from RiboEx (GeneAll, Korea) and SYBR Green master mix and cDNA synthesis kits were procured from Ampliqon A/S (Odense M, Denmark) and CinaColon (Tehran, Iran), respectively. 3-(4,5-dimethylhiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Trypan blue and Trypsin-EDTA were all purchased from from INOCLON (G. Innovative Biotech Co (INOCLON), Iran).
The A2780s and A2780cp ovarian cancer cells were procured from Pasteur Institute of Iran (Tehran, Iran) and incubated in RPMI1640 (Gibco, Thermofi sher, USA) supplemented with 10 % inactivated fetal Bovine serum (Gibco, Thermofi sher, USA), 100 U/mL penicillin (Sigma-Aldrich, St. Louis, MO), 100 U/mL streptomycin (Sigma-Aldrich, St. Louis, MO) at 37 °C in 5 % CO 2 and 95 % humidity. The A2780cp cell line was treated with cisplatin (1 μM) up to three passages before conducting the following assays. Cells treated with DMSO (up to 2 %) were considered as control group.

Zarei S., Reza J.Z., Jaliani H.Z., Hajizadeh M.R., Sargazi S, & Hosseinian H. (2019). Effects of carfilzomib alone and in combination with cisplatin on the cell death in cisplatin-sensitive and cisplatin-resistant ovarian carcinoma cell lines. Bratislavske lekarske listy, 120(6).

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