1480 wallac gamma counter

Manufactured by PerkinElmer

The 1480 Wallac Gamma Counter is a laboratory instrument designed for the detection and quantification of gamma-emitting radioactive samples. It provides accurate and reliable measurements of radioactivity levels in a wide range of sample types.

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Wallac Wizard 3″ 1480 Automatic Gamma Counter Wallac Wizard 1480 Automatic Gamma Counter Wallac Wizard 1480-011 Automatic Gamma Counter Wallac 1480 automated gamma counter Wallac 1480 Wizard3 Gamma Counter Wallac Gamma counter Wallac 1480 Gamma counter

3 protocols using 1480 wallac gamma counter

Biodistribution Analysis of Radioactive Tissues

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Following the second SPECT/CT image acquisition, a necropsy was performed and samples from 11 tissues were taken for radioactivity biodistribution analyses. The tissue samples were placed in tared vials and the radioactivity measured using an automated Wizard 3 gamma counter (1480 Wallac Gamma Counter, PerkinElmer). Data were decay-corrected to the time of injection and expressed as % injected dose per gram of tissue (%ID/g).

Kennel S.J., Stuckey A., McWilliams-Koeppen H.P., Richey T, & Wall J.S. (2016). Tc-99m Radiolabeled Peptide p5+14 is an Effective Probe for SPECT Imaging of Systemic Amyloidosis. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 18(4), 483-489.

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SPECT/CT Biodistribution Measurement

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To support the SPECT/CT data, biodistribution measurements were performed as described previously^{43 (link)}. Briefly, from every mouse that was imaged with ¹²⁵I-ATRAM, samples of heart, kidneys, intestines, spleen, liver, pancreas, and stomach were harvested post mortem. Each sample was weighed, and the ¹²⁵I radioactivity was measured using an automated Wizard 3 gamma counte^{44 (link)}r (1480 Wallac Gamma Counter, Perkin Elmer).

Nguyen V.P., Palanikumar L., Kennel S.J., Alves D.S., Ye Y., Wall J.S., Magzoub M, & Barrera F.N. (2019). Mechanistic insights into the pH-dependent membrane peptide ATRAM. Journal of controlled release : official journal of the Controlled Release Society, 298, 142-153.

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Comparative Biodistribution of Radiolabeled Peptides

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For comparison of the p5+14 and p5 peptides, mice were injected IV in the lateral tail vein with a mixture of ¹²⁵I-p5+14 (150 μCi, ~3 μg) and ^99mTc-p5 (300 μCi, ~8 μg) and the mice euthanised by an overdose of isoflurane anaesthetic 1 h post injection. Tissues were harvested at necropsy for biodistribution measurements. Organs were also harvested from mice injected with ¹²⁵I-SAP and ^99mTc-p5+14 after SPECT/CT imaging. A small volume of tissue from each mouse was placed into a tared plastic vial and weighed. The radioactivity in the low and high energy windows (¹²⁵I and ^99mTc, respectively) was measured using an automated Wizard 3 gamma counter (1480 Wallac Gamma Counter, Perkin Elmer) with a crossover correction from the high to low energy channel of 4.6%, applied manually. Biodistribution data were expressed as percent injected dose per gram (%ID/g) of tissue.

Martin E.B., Williams A., Richey T., Stuckey A., Heidel R.E., Kennel S.J, & Wall J.S. (2016). Comparative evaluation of p5+14 with SAP and peptide p5 by dual-energy SPECT imaging of mice with AA amyloidosis. Scientific Reports, 6, 22695.

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