Anti mouse dynabeads

Manufactured by Thermo Fisher Scientific

Anti-mouse Dynabeads are magnetic beads coated with antibodies specific to mouse proteins. They are designed for the rapid and efficient isolation and purification of mouse target proteins and other biomolecules from complex biological samples.

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Lab products found in correlation

Ampure bead, by Beckman Coulter (2 mentions) Bioruptor standard ucd 200, by Diagenode (1 mentions) Dynabead, by Thermo Fisher Scientific (1 mentions) Ultra pure anti biotinmicrobeads, by Miltenyi Biotec (1 mentions) Easysep human pan dc pre enrichment kit, by STEMCELL (1 mentions) Ls column, by Miltenyi Biotec (1 mentions) Anti cd8β yts156.7.7, by BioLegend (1 mentions) Anti cd4 gk1, by BioLegend (1 mentions) 70 μm cell strainer, by BD (1 mentions)

Spelling variants of this product

Dynabeads (2112 citations) Dynabeads Sheep anti-Mouse IgG (19 citations) Dynabeads Goat Anti-Mouse IgG (9 citations) Mouse T-Activator anti-CD3/CD28 Dynabeads (8 citations) Mouse anti-CD3/28 Dynabeads (5 citations) Anti-mouse CD3/CD28 Dynabeads (3 citations) Sheep anti-mouse IgG magnetic Dynabeads (3 citations) Dynabead M-280 Sheep Anti-Mouse IgG (2 citations) Magnetic anti-mouse protein A dynabeads (2 citations) Anti-Mouse immunoglobulin Dynabeads (2 citations)

7 protocols using anti mouse dynabeads

DNA Methylation Profiling in Spermatogenic Cells

Cited 2 times

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5mC-, 5hmC, and 5caC-DNA IP (DIP) was performed as described^{14 (link),43 (link)}. Spermatogenic cells were purified using FACS sorting^{31 (link)}. Fractions were assessed under phase optics and the purity of rST fraction was consistently more than 95%. Genomic DNA was isolated from rST and SZ cells or hPSCs according to standard procedures and fragmented to 100–300 bp using Diagenode Bioruptor Standard UCD-200. 10 μg of genomic DNA was used for immunoprecipitation. 5hmC- and 5caC-DIP were carried out using rabbit polyclonal antibodies (Active Motif) and magnetic anti-rabbit Dynabeads (Invitrogen). Mouse monoclonal antibody (clone 33D3, Diagenode) and anti-mouse Dynabeads (Invitrogen) were used for 5mC-DIP. Specificity of the antibodies was assessed in DIP experiments with modified oligonucleotides as described^{14 (link)}.

Blythe M.J., Kocer A., Rubio-Roldan A., Giles T., Abakir A., Ialy-Radio C., Wheldon L.M., Bereshchenko O., Bruscoli S., Kondrashov A., Drevet J.R., Emes R.D., Johnson A.D., McCarrey J.R., Gackowski D., Olinski R., Cocquet J., Garcia-Perez J.L, & Ruzov A. (2021). LINE-1 transcription in round spermatids is associated with accretion of 5-carboxylcytosine in their open reading frames. Communications Biology, 4, 691.

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DRIP-qPCR for R-loop Enrichment

Cited 1 time

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DRIP-qPCR was done as previously described (38 (link)) with the following changes. Genomic DNA was extracted from ∼3 million VCaP cells, immunoprecipitated with 8 μg of S9.6 antibody and 100 μl anti-mouse Dynabeads (Invitrogen), and DNA cleaned up using AMPure beads (Beckman Coulter Inc.). Enrichment is reported as percent IP/Input.

Nicholas T.R., Metcalf S.A., Greulich B.M, & Hollenhorst P.C. (2021). Androgen signaling connects short isoform production to breakpoint formation at Ewing sarcoma breakpoint region 1. NAR Cancer, 3(3), zcab033.

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Enrichment of CD135+ and Human Myeloid Cells

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Mouse cell suspensions were incubated with anti-CD135-biotin (Biolegend)
for 30 min at 4°C, washed and incubated with Ultra-Pure anti-Biotin
microbeads (Miltenyi) for 30 min at 4°C. CD135⁺ cells were
selected using LS columns (Miltenyi). Human myeloid cells were negatively
enriched from PBMCs using anti-CD3 (OKT3), anti-CD14 (HCD14), anti-CD19 (HIB19)
and anti-CD335 (9E2) Abs followed by anti-mouse Dynabeads (Invitrogen) at a
concentration of 4 beads per target cell⁵². For IL-1β experiments, human DC were negatively
enriched using EasySep Human pan-DC Pre Enrichment Kit (StemCell
Technologies).

Sulczewski F.B., Maqueda-Alfaro R.A., Alcántara-Hernández M., Perez O.A., Saravanan S., Yun T.J., Seong D., Hornero R.A., Raquer-McKay H.M., Esteva E., Lanzar Z.R., Leylek R.A., Adams N.M., Das A., Rahman A.H., Gottfried-Blackmore A., Reizis B, & Idoyaga J. (2023). TRANSITIONAL DENDRITIC CELLS ARE DISTINCT FROM CONVENTIONAL DC2 PRECURSORS AND MEDIATE PRO-INFLAMMATORY ANTIVIRAL RESPONSES. Nature immunology, 24(8), 1265-1280.

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Cap-Dependent mRNA Immunoprecipitation and Quantification

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Cap immunoselection was performed as previously described (19 (link)). Ten micrograms of anti-2,2,7-trimethylguanosine mouse K121 antibody (Calbiochem) were incubated with 50 μl of anti-Mouse Dynabeads (Invitrogen) to immunoprecipitate capped RNA from 2 μg of total RNA. A control without antibodies was processed for each sample. cDNA produced from the immunoprecipitated RNA (RNA-IP) and input samples (RNA-Input) were quantified by RT-qPCR as described above. Values from RNA-IP were normalized to data from RNA-Input and, subsequently, the results obtained were normalized to the level ofGAPDH gene mRNA. Primers to detect pre-mRNA (ChIP c-Myc +532) amplify a region inside intron 1 and primers to detect mature mRNA (ChIP c-Myc +2007) amplify inside exon 2 (see supplementary Table S1). Average and standard deviation were calculated from three independent experiments.

Jimeno-González S., Ceballos-Chávez M, & Reyes J.C. (2015). A positioned +1 nucleosome enhances promoter-proximal pausing. Nucleic Acids Research, 43(6), 3068-3078.

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Genome-Wide DRIP-qPCR in VCaP Cells

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DRIP-qPCR was done as previously described (Sanz and Chedin, 2019) with the following changes. Genomic DNA was extracted from ~3 million VCaP cells, immunoprecipitated with 8 µl of S9.6 antibody and 100ul anti-mouse Dynabeads (Invitrogen), and DNA cleaned up using AMPure beads (Beckman Coulter Inc.). Enrichment is reported as percent IP/Input.

Nicholas T.R., & Hollenhorst P.C. (2020). Androgen signaling connects short isoform production to breakpoint formation at Ewing sarcoma breakpoint region 1 via an R-loop-dependent mechanism.

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Isolation and Enrichment of Invariant Thymic Lymphocytes

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IELPs were isolated by dissecting the thymus and single-cell suspension of thymocytes was isolated by mashing the tissue through a 70-μm cell strainer (BD Biosciences, San Jose, CA) with 15 ml of ice-cold PBS. Lymphocytes of thymus were enriched by removing erythrocytes through incubation in red cell lysis buffer.
In case of further IELP enrichment, CD4⁺ and CD8⁺ thymocytes were depleted by incubation with purified anti-CD4 (GK1.5) and anti-CD8β (YTS156.7.7) antibody (both from BioLegend, San Diego, CA) and anti-mouse Dynabeads (Life Technologies, Carlsbad, CA) according to the manufacturer’s protocol.

Hummel J.F., Zeis P., Ebert K., Fixemer J., Konrad P., Schachtrup C., Arnold S.J., Grün D, & Tanriver Y. (2019). Single-cell RNA-sequencing identifies the developmental trajectory of C-Myc-dependent NK1.1− T-bet+ intraepithelial lymphocyte precursors. Mucosal Immunology, 13(2), 257-270.

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Co-Immunoprecipitation of Caveolin-3

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24–36 h after transfection, MEF-KO cells were re-suspended in co-IP buffer and supernatants were immunoprecipitated overnight at 4 °C, using anti-cav-3 antibody (Becton Dickinson). Anti-mouse Dynabeads^® (Life Technologies) were used to bind. After elution and denaturation, proteins were run on SDS-PAGE and revealed by western blotting using specific antibodies (see Supplementary material online). All co-IP runs were repeated at least twice.

Campostrini G., Bonzanni M., Lissoni A., Bazzini C., Milanesi R., Vezzoli E., Francolini M., Baruscotti M., Bucchi A., Rivolta I., Fantini M., Severi S., Cappato R., Crotti L., J. Schwartz P., DiFrancesco D, & Barbuti A. (2017). The expression of the rare caveolin-3 variant T78M alters cardiac ion channels function and membrane excitability. Cardiovascular Research, 113(10), 1256-1265.

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