Nigericin sodium salt

Primary antibodies specific for murine CD11c (clone N418), CD206 (MMR) (clone C068C2), CD115 (CSF-1R) (clone AFS98), Dectin-1 (CLEC7A) (clone RH1), MHC class I and MCH class II (I-A^b) (clone AF6-120) were from Biolegend (San Diego, CA). Ly6C (clone HK1.4) was purchased from Ebioscience and CD36 (clone CRF D2712), CD25 (clone PC61), CD4 (clone RM4-5) and CD8 (clone 53.6.7) were purchased from BD-Bioscience (San Jose, CA). DQ^™ ovalbumin, OVA-Alexa 647, Dextran Alexa Fluor^® 488 (mw 10,000) Anionic Fixable, Staphylococcus aureus (wood strain without protein A) BioParticles^®, Alexa Fluor^® 488 conjugate pHrodo^® Green Escherichia coli BioParticles^® were purchased from Molecular Probe (Eugene, Oregon). Nigericin sodium salt was purchased from Enzo Life sciences (Farmingdale, New York). Monensin sodium salt was purchased from Amresco (Solo, Ohio).

Lipopolysaccharide (LPS) derived from E. coli 055:B5 and penicillin-streptomycin solution were purchased from Sigma Aldrich (St. Louis, MO, USA). RPMI-1640 medium was purchased from Wako (Osaka, Japan). Fetal bovine serum (FBS), de Man, Rogosa, and Sharpe (MRS) broth, and Brewer's modified thioglycollate medium were purchased from BD Bioscience (Franklin Lakes, NJ, USA). Nigericin sodium salt was obtained from Enzo Life Sciences (Farmingdale, NY, USA). DNase I and pronase were purchased from Roche Diagnostics (Basel, Switzerland). RNase A was purchased from Nacalai Tesque (Kyoto, Japan).