Dynabeads M270-Epoxy (Thermo Fisher Scientific) were covalently conjugated with combinations of mouse anti-human CD3 antibody (clone UCHT1, BioLegend), recombinant human PD-L2-human IgG1 Fc chimera protein (R&D Systems), or mouse IgG1 isotype antibodies (R&D Systems) following the manufacturer's recommendations. The molar ratio between anti-human CD3 and PD-L2 Fc was 1:3, and the molar ratios of all added proteins were kept constant by the addition of mouse IgG isotype antibodies. For each preparation, we used 8 μg of total protein/1 mg of beads, of which 2 μg were anti-CD3 and 4 μg were PD-L2 Fc.
Mouse anti human cd3 antibody clone ucht1
Manufactured by BioLegend
The Mouse anti-human CD3 antibody (clone UCHT1) is a laboratory reagent used to detect the presence of the CD3 protein on the surface of human T cells. The CD3 protein is a crucial component of the T cell receptor complex and is expressed on all mature T cells. This antibody can be used for various applications, such as flow cytometry, immunohistochemistry, and Western blotting, to identify and study T cell populations.
Automatically generated - may contain errors
3 protocols using mouse anti human cd3 antibody clone ucht1
1
Conjugation of Immune Checkpoint Modulators
2
Isolation and Activation of Primary T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Jurkat T cells were obtained from the ATCC and maintained in RPMI medium supplemented with 10% FBS and 1% penicillin and streptomycin. Peripheral blood was acquired from the New York Blood Center. Total CD3+ T cells were isolated by density gradient centrifugation (Lymphoprep) and adverse selection using the RosetteSep human T cell enrichment cocktail (Stemcell). Primary T cells were directly employed in stimulation assays or maintained in culture. T cell cultures were maintained in complete RPMI, containing 10% FCS, MEM nonessential amino acids, 1 mM sodium pyruvate, 100 IU/ml of penicillin, 100 μg/ml streptomycin, and GlutaMAX-I. For stimulation, Dynabeads M270-Epoxy (Thermo) was covalently conjugated with combinations of mouse anti-human CD3 antibody (clone UCHT1, BioLegend), mouse anti-human CD28 antibody (BioLegend), recombinant human PD-L1 human IgG1 Fc chimera protein (R&D Systems), or mouse IgG1 isotype antibodies (R&D Systems) following the manufacturer’s recommendations. All Jurkat and primary T cell stimulations were performed with beads at a 1:5 cell-to-bead ratio.
3
Isolation and Stimulation of Primary T Cells
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!