CT imaging was collected from the Siemens Inveon PET/CT imaging system. For in vitro CT imaging, various Au@Cu2-xSe aqueous dispersions with concentrations of 0, 0.625, 1.25, 2.5, and 5 mg mL−1 were placed in small tubes for CT imaging. In vivo CT imaging was conducted with tumor-bearing mice (n = 3, per group) after intravenous or intratumoral injection of Au@Cu2-xSe NPs. For intravenous injection, the CT images were collected at 1, 2, 4, 6, and 8 h post-injection of 200 µL saline containing Au@Cu2-xSe NPs (2 mg mL−1). The CT images before injection were used as control. For intratumoral injection, the CT images were collected after injection of 150 µL saline containing Au@Cu2-xSe NPs with 0, 6, and 8 mg mL−1, respectively.
Inveon pet ct imaging system
Manufactured by Siemens
The INVEON PET/CT imaging system is a preclinical imaging platform designed for small animal research. It combines positron emission tomography (PET) and computed tomography (CT) technologies to provide high-resolution, multimodal imaging capabilities. The system is used for the non-invasive visualization and quantification of biological processes in small animal models.
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4 protocols using inveon pet ct imaging system
1
In vitro and in vivo CT imaging of Au@Cu2-xSe NPs
2
Micro-PET Imaging of Mice with 18F-FDG Tracer
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Before experiments, each mouse (7.5 months, 28~32 g) for blood glucose monitoring, the results showed the normal range (7.0~10.1 mmol/L) could be used for micro-PET detective (18F-FDG PET tracer was provided by the Chinese Medicine Research Institute PET Room; PET imaging system using Siemens INVEON PET/CT imaging system). Six hours of water deprivation before the experiment. The mice were placed in the suction chamber, inhaling the oxygen mixed with 1.5% isoflurane to be anesthetized. After complete anesthesia, approximately 14.8~16.5 MBq 18F-FDG PET tracers were injected via vena of tail. After the 18F-FDG PET tracer uptake for 60 min, the mice were placed on the scan bed in prone position, the mice and scanner long axis were parallel, and the head of mouse was located within the scanner field of view. Then the micropositron emission tomography began to collect the image. During this progress, the mice were anesthetized by the oxygen mixed with 1.5% isoflurane (1 L/min).
3
Mouse Micro-PET Imaging Protocol
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Four animals were randomly selected from each group for micro-PET detection. The 18F-FDG PET tracer was provided by the Chinese Medicine Research Institute PET Room; PET imaging was carried out on a Siemens INVEON PET/CT imaging system. Before the experiments, mice (7.5 months, 28~32 g) were submitted to blood glucose monitoring and showed levels in the normal range (7.0~10.1 mmol/L). Therefore, they could be assessed by micro-PET. Mice were deprived of water 6 h before assessment. The animals were placed in the suction chamber, inhaling oxygen mixed with 1.5% isoflurane for anesthesia. After complete anesthesia, approximately 14.8~16.5 MBq18F-FDG PET were injected via the tail vein. After 18F-FDG PET tracer uptake for 60 min, the mice were placed in the prone position, parallel to the scanner long axis, with the head located within the scanner field of view. Then, micropositron emission tomography began to collect images. The mice were anesthetized by inhalation of oxygen mixed with 1.5% isoflurane (1 L/min).
4
Micro-PET Brain Imaging in Mice
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Four animals were randomly selected from each group for micro-PET detection using 18 F-fluorodeoxyglucose ( 18 F-FDG) PET tracers provided by the Chinese Medicine Research Institute PET Room and a Siemens INVEON PET/CT imaging system. Before experiments began, the 20 mice underwent measurement of blood glucose, which was consistently within the normal range (~7.0-10.1 mmol/L). Following a 6 hour fast, the mice were placed into a suction chamber. Once completely anaesthetised using inhaled oxygen mixed with 1.5% isoflurane (1 L/min), approximately ~14.8-16.5 MBq 18 F-FDG PET tracer was injected via the tail vein. After allowing 18 F-FDG PET tracer uptake for 60 min, the mice were placed on the scan bed in a prone position, parallel to the long axis of the scanner, with their heads positioned within the scanner's field of view. Micro-PET images were acquired at a frame rate of 30 s/frame and reconstructed using filtered back projection (FBP) and CT photon attenuation correction. Three-dimensional region of interest technology was applied to examine the whole brain (cortex, frontal lobe and hippocampus) in transverse, coronal and sagittal planes. The uptake rate per gram (%ID/g) was calculated within this region of interest.
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