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Pgl3 luciferase reporter vectors technical manual

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The PGL3 Luciferase Reporter Vectors Technical Manual provides detailed information on the use and properties of the PGL3 Luciferase Reporter Vectors. The manual covers the vector design, cloning sites, and other technical specifications required for the effective utilization of these reporter vectors.

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Dual glo luciferase assay system, by Promega (2 mentions)

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PGL3 vector (739 citations) PGL3 luciferase reporter vector (312 citations) PGL3 luciferase vector (134 citations) PGL3 reporter vector (65 citations) Luciferase reporter vector (49 citations) Reporter vectors (3 citations)

2 protocols using pgl3 luciferase reporter vectors technical manual

1

Luciferase Assay in THP-1 Cells

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Luciferase reporter assay was performed exactly according to the manufacture’s manual (Promega, pGL3 Luciferase Reporter Vectors Technical Manual). 0.1 μg of pGL3 expression vector was co-transfected with the control vector pRL by TransIT-2020 (Muris) into 4.5×104 THP-1 cells in a well of a 96 well plate. After 48 hr incubation, luciferase activity was measured with the Dual-Glo luciferase assay system (Promega).
Li G., Martínez-Bonet M., Wu D., Yang Y., Cui J., Nguyen H.N., Cunin P., Levescot A., Bai M., Westra H.J., Okada Y., Brenner M.B., Raychaudhuri S., Hendrickson E.A., Maas R.L, & Nigrovic P.A. (2018). High Throughput Identification of Non-Coding Functional SNPs via Type IIS Enzyme Restriction. Nature genetics, 50(8), 1180-1188.
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2

Luciferase Assay in THP-1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Luciferase reporter assay was performed exactly according to the manufacture’s manual (Promega, pGL3 Luciferase Reporter Vectors Technical Manual). 0.1 μg of pGL3 expression vector was co-transfected with the control vector pRL by TransIT-2020 (Muris) into 4.5×104 THP-1 cells in a well of a 96 well plate. After 48 hr incubation, luciferase activity was measured with the Dual-Glo luciferase assay system (Promega).
Li G., Martínez-Bonet M., Wu D., Yang Y., Cui J., Nguyen H.N., Cunin P., Levescot A., Bai M., Westra H.J., Okada Y., Brenner M.B., Raychaudhuri S., Hendrickson E.A., Maas R.L, & Nigrovic P.A. (2018). High Throughput Identification of Non-Coding Functional SNPs via Type IIS Enzyme Restriction. Nature genetics, 50(8), 1180-1188.
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