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Bioanalyzer with nano total rna chips

Manufactured by Agilent Technologies
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Sourced in United States

The Bioanalyzer with Nano total RNA Chips is a lab equipment product that provides automated, high-resolution analysis of RNA samples. The core function of this product is to assess the quality and quantity of total RNA samples using microfluidic technology.

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Lab products found in correlation

Genechip wt pico reagent kit, by Thermo Fisher Scientific (2 mentions) Genechip mouse st arrays 2, by Thermo Fisher Scientific (2 mentions) Rneasy micro kit, by Qiagen (2 mentions)

2 protocols using bioanalyzer with nano total rna chips

1

Transcriptomic Profiling of Microglia Subsets

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from the eKC and mKC sorted from Cx3cr1-CreERT2R26-tdT mice with the RNeasy Micro Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocol. Quality and quantity of RNA was estimated with Bioanalyzer with Nano total RNA Chips (Agilent, Santa Clara, CA, USA), the RIN values exceeded 7.8 for all samples used for microarray measurement. Total RNA was amplified, fragmented and labeled with biotin using the GeneChip WT Pico Reagent Kit (Affymetrix, Santa Clara, CA, USA) and samples were hybridized to GeneChip Mouse ST Arrays 2.0 according to the manufacturer’s instructions. Probeset signal intensities were background-corrected and normalized using the RMA algorithm. Significantly regulated genes were identified using paired-T tests (pairing by animal, limma package of R/Bioconductor) with Benjamini-Hochberg correction for multiple testing. Genes regulated at least 1.5 fold with p < 0.1 were considered significant. The microarray analysis was done with CARMAweb 1.5 software38 (link).
Theurl I., Hilgendorf I., Nairz M., Tymoszuk P., Haschka D., Asshoff M., He S., Gerhardt L.M., Holderried T.A., Seifert M., Sopper S., Fenn A.M., Anzai A., Rattik S., McAlpine C., Theurl M., Wieghofer P., Iwamoto Y., Weber G.F., Harder N.K., Chousterman B.G., Arvedson T.L., McKee M., Wang F., Lutz O.M., Rezoagli E., Babitt J.L., Berra L., Prinz M., Nahrendorf M., Weiss G., Weissleder R., Lin H.Y, & Swirski F.K. (2016). On-demand erythrocyte disposal and iron recycling requires transient macrophages in the liver. Nature medicine, 22(8), 945-951.
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2

Transcriptomic Profiling of Microglia Subsets

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from the eKC and mKC sorted from Cx3cr1-CreERT2R26-tdT mice with the RNeasy Micro Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocol. Quality and quantity of RNA was estimated with Bioanalyzer with Nano total RNA Chips (Agilent, Santa Clara, CA, USA), the RIN values exceeded 7.8 for all samples used for microarray measurement. Total RNA was amplified, fragmented and labeled with biotin using the GeneChip WT Pico Reagent Kit (Affymetrix, Santa Clara, CA, USA) and samples were hybridized to GeneChip Mouse ST Arrays 2.0 according to the manufacturer’s instructions. Probeset signal intensities were background-corrected and normalized using the RMA algorithm. Significantly regulated genes were identified using paired-T tests (pairing by animal, limma package of R/Bioconductor) with Benjamini-Hochberg correction for multiple testing. Genes regulated at least 1.5 fold with p < 0.1 were considered significant. The microarray analysis was done with CARMAweb 1.5 software38 (link).
Theurl I., Hilgendorf I., Nairz M., Tymoszuk P., Haschka D., Asshoff M., He S., Gerhardt L.M., Holderried T.A., Seifert M., Sopper S., Fenn A.M., Anzai A., Rattik S., McAlpine C., Theurl M., Wieghofer P., Iwamoto Y., Weber G.F., Harder N.K., Chousterman B.G., Arvedson T.L., McKee M., Wang F., Lutz O.M., Rezoagli E., Babitt J.L., Berra L., Prinz M., Nahrendorf M., Weiss G., Weissleder R., Lin H.Y, & Swirski F.K. (2016). On-demand erythrocyte disposal and iron recycling requires transient macrophages in the liver. Nature medicine, 22(8), 945-951.
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