Anti apoa1 antibody

Manufactured by Santa Cruz Biotechnology

The Anti-ApoA1 antibody is a laboratory product that can be used to detect and measure the levels of ApoA1 (Apolipoprotein A-I) in biological samples. ApoA1 is a major component of high-density lipoprotein (HDL) cholesterol and plays a critical role in cholesterol metabolism and transport. The Anti-ApoA1 antibody can be utilized in various immunoassay techniques, such as ELISA, to quantify ApoA1 concentrations in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd63 antibody, by Santa Cruz Biotechnology (1 mentions) Anti claudin 5 antibody, by Thermo Fisher Scientific (1 mentions) Anti alix antibody, by Cell Signaling Technology (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) Na931, by GE Healthcare (1 mentions) Na934, by GE Healthcare (1 mentions) Anti tsg101 antibody, by Santa Cruz Biotechnology (1 mentions) Anti occludin antibody, by Thermo Fisher Scientific (1 mentions) Anti ldlr antibody, by Abcam (1 mentions) Supersignal west substrate, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions)

Close spelling variants of this product

ApoA1 (9 citations)

2 protocols using anti apoa1 antibody

Western Blot Analysis of Extracellular Vesicle Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein samples were loaded onto 4–12% Tris-glycine gels. After electorophoresis and transferring to nitro-cellulose membranes, the membranes were blocked in Tris-buffered saline containing 5% no-fat milk for 60 min at room temperature. Membranes were then incubated overnight at 4 °C with anti-Ascl1 antibody (1:100, Santa Cruz Biotechnology, sc-374104), anti-CD63 antibody (1:200, Santa Cruz Biotechnology, sc-5275), anti-Alix antibody (1:1000, Cell Signaling Technology, 2171), anti-TSG101 antibody (1:200, Santa Cruz Biotechnology, sc-7964), anti-ApoA1 antibody (1:200, Santa Cruz Biotechnology, sc-69755), anti-HRS antibody (1:1000, Santa Cruz Biotechnology, sc-271455), anti-Occludin antibody (1:500, Invitrogen, 71-1500), anti-Claudin-5 antibody (1:1000, Invitrogen, 35-2500) and anti-β-actin antibody (1:2000, Sigma-Aldrich, A5441). After incubation with peroxidase-conjugated secondary antibodies, visualization was enhanced by chemiluminescence (GE Healthcare, NA931- anti-mouse, or NA934- anti-rabbit). Optical density was assessed using the ImageJ. Uncropped versions of the blots are shown in the Source Data.

Li W., Mandeville E.T., Durán-Laforet V., Fukuda N., Yu Z., Zheng Y., Held A., Park J.H., Nakano T., Tanaka M., Shi J., Esposito E., Niu W., Xing C., Hayakawa K., Lizasoain I., van Leyen K., Ji X., Wainger B.J., Moro M.A, & Lo E.H. (2022). Endothelial cells regulate astrocyte to neural progenitor cell trans-differentiation in a mouse model of stroke. Nature Communications, 13, 7812.

+ Open protocol

+ Expand

Immunoblotting of Liver Protein Targets

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Approximately 50 mg of frozen liver tissue was homogenized in RIPA buffer containing 1 mM PMSF and protease inhibitor cocktail (Roche). After protein quantitation using BCA protein assay reagent (Pierce), 50 μg of homogenate proteins from individual liver samples were resolved by SDS-PAGE and transferred to nitrocellulose membranes. Anti-LDLR antibody was purchase from BioVision (Mountain View, CA). Anti-hamster PCSK9 antibody that recognizes the C-terminal end of hamster PCSK9 (CRNRPSAKASWVHQ) was developed in our laboratory and previously reported [27 (link)]. Anti-mouse PCSK9 antibody was obtained from R&D System. Anti-actin and anti-V5 antibodies were purchased from Sigma-Aldrich. Anti-ApoB antibody was a gift from Dr. Khosrow Adeli (Hospital for Sick Children, University of Toronto, Canada). Anti-ApoA1 antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Immunoreactive bands of predicted molecular mass were visualized using SuperSignal West Substrate (Thermo Scientific) and quantified with the Alpha View Software with normalization by signals of β-actin.

Dong B., Singh A.B., Azhar S., Seidah N.G, & Liu J. (2015). High-fructose feeding promotes accelerated degradation of hepatic LDL receptor and hypercholesterolemia in hamsters via elevated circulating PCSK9 levels. Atherosclerosis, 239(2), 364-374.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!