Human CRC cells (LoVo, RKO, SW480, HCT116, and HT-29) and normal colon mucosa cells (NCM460) purchased from ATCC were kept in RPMI-1640 with 10% FBS (Gibco) at 37°C. The pcDNA3.1-ADAMTS9-AS2, pcDNA3.1-FTO and pcDNA3.1-YTHDF2 plasmids, the small interfering RNAs (siRNAs) specific for BTG2 (si-BTG2) and ADAMTS9-AS2 (si-ADAMTS9-AS2), and short hairpin (sh) RNA for YTHDF2 (sh-YTHDF2) were obtained from GenePharma (Suzhou). Hsa-miR-27a-3p mimics/inhibitors/negative control (NC) were obtained from GenePharma. Lipo2000 was adopted for transfections.
Pcdna3.1 fto
Manufactured by GenePharma
Sourced in China
PcDNA3.1-FTO is a plasmid vector for gene expression. It contains the FTO (fat mass and obesity-associated) gene as the insert. The FTO gene is known to be involved in the regulation of body weight and metabolism. The vector provides a platform for the expression and study of the FTO gene in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Sh ythdf2, by GenePharma (1 mentions)
Ncm460, by American Type Culture Collection (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Sw480, by American Type Culture Collection (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Ht 29, by American Type Culture Collection (1 mentions)
Plv circ luci 2a puro, by GenePharma (1 mentions)
Si fto, by GenePharma (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
2 protocols using pcdna3.1 fto
1
Functional Characterization of CRC Pathways
2
Manipulating circGPR137B and FTO in HCC
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Lentivirus mediated circGPR137B overexpression vector (pLV-circGPR137B) and empty vector pLV-circ-Luci (2A) puro (CON), plasmids pcDNA3.1-FTO, si-FTO, shRNA target sequence for circGPR137B (sh-circGPR137B: 5′-ATGTTCACAGAGTGATTTTCA-3′) and miR-4739 mimic or inhibitors were purchased from GenePharma (Shanghai, China). Negative control (NC), sh-NC, pcDNA3.1 or miR-NC was used as the control vectors. HCC cell lines were planted in 6-well plates 24 h prior to sh-circGPR137B, circGPR137B or miR-4739 transfection with 70% confluence, and then treated with Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).
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