The viable cell mass was measured with the Cell Counting Kit-8 (CCK8) Assay (Bimake, Houston, TX, United States). The CC stem cells (1 × 105) were transfected with miR-92a-3p mimic/inhibitor and seeded in 96-well plates. The cells were then cultured in an incubator with 5% CO2 at 37 °C for 1, 2, 3, 4, and 5 days respectively. Totally 10 μl CCK8 solution was added into each well, and the cells were cultured for another 2 h. The absorbance was finally determined at 490 nm with a microplate reader.
Cell counting kit 8 cck 8 assay
Manufactured by Selleck Chemicals
Sourced in United States
The Cell Counting Kit-8 (CCK-8) assay is a colorimetric assay that can be used to determine the number of viable cells in a sample. The assay is based on the reduction of a water-soluble tetrazolium salt, WST-8, to a yellow-colored formazan product by the dehydrogenase activity of viable cells. The amount of the formazan product generated is directly proportional to the number of living cells, which can be measured using a spectrophotometer.
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7 protocols using cell counting kit 8 cck 8 assay
1
Cell Viability Assay using CCK8
2
Cell Viability Assay with ZnO NPs
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Cell viability was determined by the Cell Counting Kit-8 (CCK-8) Assay (Bimake, USA). Briefly, cells were seeded in 96-well culture plates at a density of 5 × 103/well for 24 h. For determination of the viability after exposure to 15 μg/mL ZnO NPs for the indicated times, detection reagent, made up of fresh medium containing 10% kit reagent, was added to cells and followed by 1–4 h incubation. The absorbance was measured using the ELx800 microplate reader (BioTek Instruments, USA) at a wavelength of 450 nm.
3
Cell Viability and Synergy Evaluation
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Cells were seeded into 96‐well plates at an appropriate density per well. Cell viability was calculated by the Cell Counting Kit‐8 (CCK‐8) assay (Bimake, TX, USA) at different time points. The absorbance values were detected by microplate reader (BioTek, VT, USA) at a wavelength of 450 nm. The combination index (CI) was calculated using the CompuSyn software based on the Chou–Talalay methodology, and less than 1 indicates synergy.16
4
Cell Viability Assay with CCK-8
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Cell viability was assessed with the Cell Counting Kit-8 (CCK-8) assay (http://Bimake.com , Houston, TX, USA) according to the manufacturer's instructions. The cells were seeded at a density of 5000 cells/well in 24-well plates and cultured for 1–6 days. At the end of each time interval, cell samples were washed with PBS and incubated with serum-free medium containing 10% CCK-8 reagent. After 4 h of incubation at 37°C in an atmosphere of 5% CO2, aliquots were pipetted into a 96-well plate and measured at 450 nm using an enzyme-labeling instrument (Thermo Fisher Scientific).
5
Cell Viability Assay and Drug Combination Analysis
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Cell viability was assessed using a Cell Counting Kit-8 (CCK-8) assay (Bimake, USA) according to the manufacturer’s instructions. Cells were seeded into 96-well plates at 6,000 cells/well and cultured for 24 h. The 96-well plate contained with small molecule drugs was incubated for 18, 24, 36, and 48 h. Finally, the absorbance of each well was measured using a spectrophotometer (Beckman, USA) at an emission wavelength of 450 nm. The combination index (CI) scores for Loewe additivity were acquired using the Ting Chao Chou CI-isobologram equation applied in CompuSyn (http://www.combosyn.com/ ) [16 (link)].
6
Evaluating Glioblastoma Cell Viability
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Cell viability was evaluated using the Cell Counting Kit-8 (CCK8) assay (Bimake, Houston, TX, USA), according to the manufacturer's instructions. Glioblastoma cells were seeded in a 96-well plate at a density of 8 × 103 cells/well, cultured for 24 h, and treated with the indicated compounds. The optical density (OD) value was measured at 450 nm using a microplate reader BioTek ELX800 (Gene Co., Ltd., Shanghai, China).
7
Cell Viability Assessment via CCK-8 Assay
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Cell viability was assessed using a Cell Counting Kit-8 (CCK-8) assay (Bimake, USA) according to the manufacturer’s instructions. Cells were seeded into 96-well plates at 3000 cells/well and cultured for 24, 48, 72, or 96 h. Then, 10 μL of CCK-8 solution was added to each well, and the 96-well plate was incubated for 2 h at 37 °C and 5% CO2. The fluorescence of each plate was measured using a spectrophotometer at an emission wavelength of 450 nm (Beckman, USA). Six replicates per sample were analyzed.
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