Nunc cryotube vial

Manufactured by Thermo Fisher Scientific

Sourced in Denmark

Nunc™ CryoTube™ Vials are sterile, single-use containers designed for cryogenic storage of biological samples. They are made from high-quality polypropylene and are available in various sizes to accommodate different sample volumes.

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Lab products found in correlation

Bovine serum albumin (bsa), by Merck Group (2 mentions) Spermfreeze solution, by Vitrolife (1 mentions) Pyruvate, by Merck Group (1 mentions) Ficoll 70, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Sucrose, by Merck Group (1 mentions) Mr frosty freezing container, by Thermo Fisher Scientific (1 mentions) Vacuette lithium heparin tubes, by Greiner (1 mentions)

Close spelling variants of this product

Nunc Cryotubes Vials CryoTube vials Nunc cryotubes Nunc vials Cryotubes NuncTM

6 protocols using nunc cryotube vial

Sperm Cryopreservation Using Sperm-Freeze Solution

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The freezing procedure was modified from the SpermFreeze Solution guideline (REF 10137; Vitrolife, Vastra Frolunda, Sweden). Sperm-Freeze Solution is a medium consisting of bicarbonate and MOPS buffered with glycerol. Glycerol acts as a permeating cryoprotectant agent (CPA) in the freezing process. This CPA protects the sperm against thermal shock [17 (link)].
SpermFreeze Solution was added slowly by drops to the sample in a 1.8 mL Nunc Cryotube vial (catalog No. 375418; Thermo Fisher Scientific, Jiangsu, China) at a ratio of 1:1 and gently mixed after each drop was added. The process took 10 minutes at room temperature. Next, the Cryotube was placed horizontally 3 cm above the liquid nitrogen surface to minimize the heat difference between the two ends and to increase the surface area in contact with nitrogen vapors. During this process, there is a thermal gradient, and the freezing temperature was estimated to be approximately −70°C, −80°C, and −99°C [10 (link)]. After 15 minutes, the Cryotube was submerged into liquid nitrogen and then stored in a tank at −196°C for at least 1 month before thawing.

Le M.T., Nguyen T.T., Nguyen T.T., Nguyen T.V., Nguyen T.A., Nguyen Q.H, & Cao T.N. (2019). Does conventional freezing affect sperm DNA fragmentation?. Clinical and Experimental Reproductive Medicine, 46(2), 67-75.

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Cryopreservation of Human Semen

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The liquefied ejaculated semen was diluted with sperm freezing media (ORIGIO, Måløv, Denmark) (ratio 1:1), and 1 ml of the suspension was pipetted into a Nunc cryotube vial (1.8 ml; catalog number: 375418, Thermo Fisher Scientific, Jiangsu, China), which was kept at room temperature (RT) for 10 min and subsequently placed in a horizontal position in the freezer. Some samples were transferred to liquid nitrogen 24 h later. The purpose of placing the container in a horizontal position was to minimize the heat difference between the two ends during freezing (Di Santo et al., 2012 (link)).

Wang X., Lu F., Bai S., Wu L., Huang L., Zhou N., Xu B., Wan Y., Jin R., Jiang X, & Tong X. (2022). A Simple and Efficient Method to Cryopreserve Human Ejaculated and Testicular Spermatozoa in −80°C Freezer. Frontiers in Genetics, 12, 815270.

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Vitrification and Thawing of Ovarian Cortex

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Ovarian cortical pieces were used for the vitrification process and subsequent thawing as described earlier [36 (link)] with slight modifications. In summary, the cortical pieces were immersed twice in vitrification solution, containing 40% ethylene glycol (EG, v/v; ThermoFisher Scientific); 30% Ficoll 70 (w/v; Sigma-Aldrich); and 0.5 M sucrose (Sigma-Aldrich), supplemented with 10 mg/ml bovine serum albumin (BSA, Sigma-Aldrich), for 2 and 3 min at room temperature (RT), respectively, transferred into Nunc™ CryoTube™ Vials (ThermoFisher Scientific) and immersed for storage in liquid nitrogen for 2–4 weeks.
For the thawing procedure, the cryovials were removed from the liquid nitrogen, allowed to equilibrate for 30 s at RT, and then incubated in a water bath at 37 °C until melted. The thawed tissue pieces were then placed into decreasing concentrations of thawing solution (1 M and 0.5 M sucrose; Sigma-Aldrich) diluted in basic solution (Phosphate-buffered saline, PBS-/-; ThermoFisher Scientific) supplemented with Glucose (Sigma-Aldrich) and Pyruvate (Sigma-Aldrich) for 2 and 3 min, respectively, and washed in a basic solution containing 10 mg/ml of BSA (Sigma-Aldrich) for 5 min.

Deligiannis S.P., Kask K., Modhukur V., Boskovic N., Ivask M., Jaakma Ü., Damdimopoulou P., Tuuri T., Velthut-Meikas A, & Salumets A. (2024). Investigating the impact of vitrification on bovine ovarian tissue morphology, follicle survival, and transcriptomic signature. Journal of Assisted Reproduction and Genetics, 41(4), 1035-1055.

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Retinal Dissection and Preservation for RNA Analysis

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Rats were deeply anesthetized with isoflurane and decapitated. Some retinas were dissected using curved forceps placed behind the eye and used to push the retina through an incision across the cornea as previously described (28 ). However, to ensure separation of the retina from the RPE, other retinas were dissected by removing the cornea, followed by extracting the lens, clearing the vitreous, and separating the retina from the RPE. Isolated retinas were frozen immediately in individual 1.8mL Nunc® CryoTube® Vials (Thermo Scientific; Denmark) in liquid N₂ and stored at −80°C until used for RNA isolation.

Dreffs A., Henderson D., Dmitriev A.V., Antonetti D.A, & Linsenmeier R.A. (2018). Retinal pH and acid regulation during metabolic acidosis. Current eye research, 43(7), 902-912.

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Cryopreservation of Natural Killer Cells

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Cells were centrifuged and resuspended at a final concentration of 1–2.5 × 10⁷ cells/mL in 50% RPMI/40% FBS/10% DMSO or a FBS-free, GMP-compliant commercial cryomedia formulation. The cell suspension was aliquoted into Nunc CryoTube Vials (Thermo Fisher Scientific) and immediately transferred to a Mr. Frosty™ Freezing Container (Thermo Fisher Scientific) and placed into -80°C overnight. Cryovials were then stored in liquid nitrogen until use. Both cryomedia formulations supported the cryopreservation of NK cells that resulted in similar recovery from thaw of NK cells that maintained the expression of activation markers post-thaw and cytotoxicity (Supplemental Figure 1). In vitro experiments were performed with NK cells cryopreserved in the commercial media formulation, and in vivo experiments were performed with NK cells cryopreserved in 50% RPMI/40% FBS/10% DMSO.

Oyer J.L., Croom-Perez T.J., Dieffenthaller T.A., Robles-Carillo L.D., Gitto S.B., Altomare D.A, & Copik A.J. (2022). Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo. Frontiers in Immunology, 13, 861681.

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Blood Sample Handling Protocol

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Blood samples were collected in Vacuette^® lithium-heparin tubes (Greiner Bio-one, Austria), centrifuged at +4°C and 2600 g for 10 min and aliquoted in polypropylene Nunc Cryotube™ vials (Thermo Scientific, Denmark) within 60 min from collection. MD samples were collected in microvials (M Dialysis) and stored at approximately −20°C. At the end of each study day all samples were transferred to a −80°C freezer until further analysis.

Wulkersdorfer B., Bergmann F., Amann L., Fochtmann-Frana A., Al Jalali V., Kurdina E., Lackner E., Wicha S.G., Dorn C., Schäfer B., Ihra G., Rath T., Radtke C, & Zeitlinger M. (2023). Effect of albumin substitution on pharmacokinetics of piperacillin/tazobactam in patients with severe burn injury admitted to the ICU. Journal of Antimicrobial Chemotherapy, 79(2), 262-270.

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