Inveon small animal pet ct

All radiotracers were administered via tail vein injection in < 300 μL PBS. Intact male C57BL6/J or nu/nu mice were treated with vehicle or mife. (25 mg/kg) via oral gavage for four days prior to injection with ∼11 MBq of ¹⁸F-GR02. Mice were anesthetized and imaged with a Siemens Inveon small animal PET/CT at dedicated time points post injection. Each mouse was imaged until 20 million coincident events were collected. All imaging data was reconstructed, decay corrected, and analyzed with AMIDE or ASIPro software. Maximum intensity projections were generated with ASIPro software. ASIPro software was also used to draw manual two dimensional regions of interest to calculate SUV data for static or dynamic acquisitions.

Mice were intravenous injected with 100 μCi of ⁶⁴Cu-BMV101 and imaged after 1 h, 2 h, and 4 h by using Inveon small-animal PET/CT (Siemens). Briefly, a CT anatomic image scan was acquired (80 kV, 500 μA) with a pixel size of approximately 0.1 mm. After CT imaging, whole-body PET imaging was performed with 5 min static scan. The PET images were reconstructed using the ordered-subsets expectation maximization 3-dimensional algorithm based on CT attenuation and analyzed using the Inveon Research Workplace (IRW) software (Siemens). PET voxel size was 0.796 × 0.861 × 0.861 mm, for a total of 128 × 128 × 159 voxels. After the 4 h PET/CT imaging, the mouse was injected with 100 μL BMV101 (10 mM) and imaged for fluorescence signal by IVIS200 (Perkin Elmer) at 1 h later. Then PET/CT quantify assay was performed, and tissue radioactivity was calculated and expressed as decay-corrected percentage injected dose per gram of tissue (%ID/g). Investigators conducting the trial were blinded as to what groups were being imaged.