Oxidized glutathione

KOD-plus-neo DNA polymerase was from Toyobo (Osaka, Japan). In-Fusion advantage PCR cloning kit was from Takara Bio (Otsu, Japan). QuikChange site-directed mutagenesis kit was from Agilent Technologies (Santa Clara, CA, USA). PureYield plasmid miniprep kit was from Promega (Tokyo, Japan). RYTS cell-free transcription-translation kit was from Roche Diagnostics (Tokyo, Japan). Oxidized glutathione was from Wako pure chemicals (Osaka, Japan). Ni Sepharose High Performance His beads were from GE healthcare (Piscataway, NJ, USA). Nanosep Centrifugal-3 k Ultrafiltration Device was from Pall Corporation (Ann Arbor, MI, USA). Immunoblock was from DS Pharma (Osaka, Japan). Anti-His-HRP antibody was from Qiagen (Hilden, Germany). 3,3′,5,5′-Tetramethylbenzidine (TMBZ), bovine serum albumin (BSA), and HEL were from Sigma (St. Louis, MO, USA). Oligonucleotides were from Operon-Eurofins (Tokyo, Japan). All water used was purified using Milli-Q (Millipore, Tokyo, Japan). Other chemicals and reagents, unless otherwise indicated, were from Sigma or Wako.

Sodium chloride (NaCl), lysozyme, monosodium phosphate (NaH₂PO₄), imidazole, glycerol, reduced glutathione, oxidized glutathione, methanol, dimethyl sulfoxide (DMSO), trifluoroacetic acid (TFA), tert-butyl acetate, perchloric acid (HClO₄), hydrochloric acid (HCl), sodium carbonate, ethyl acetate, sodium sulfate, hexane, sodium hydrogen carbonate (NaHCO₃), acetone, triphenyl phosphine (Ph₃P), anhydrous dichloromethane (CH₂Cl₂), and anhydrous tetrahydrofuran (THF) were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan). 3-Bromo-tyrosine, 3-hydroxymethyl-2-methylbiphenyl, and diisopropyl azodicarboxylate (DIAD; 40% in toluene, approximately 1.9 mol L⁻¹) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Magnesium sulfate and CH₂Cl₂ were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). Deuterochloroform (CDCl₃) was purchased from Isotec, Inc. (Miamisburg, OH, USA), and N-[(9H-fluoren-9-ylmethoxy) carbonyloxy] succinimide (Fmoc-Osu) was purchased from Watanabe Chemical Industries, Ltd. (Hiroshima, Japan).