Fatty acid methyl esters, AR (C15, C17, C19 and C25), phenolic (ferulic, vanillic and syringic acids, vanillin and p-OH-benzaldehyde), tocopherol standards (α, β, δ and γ), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2-diphenyl-1-picryhydrazyl (DPPH) and 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), were purchased from Sigma–Aldrich Co. (St. Louis, MO, USA). Syringic aldehyde was supplied by Extrasynthese (Genay, France), methyl tricosanoate by Larodan (Malmö, Sweden) and K2O8S2, FeCl3 and FeSO4 were purchased from Panreac (Barcelona, Spain).
Tocopherol standard
Manufactured by Merck Group
Sourced in United States, Germany
Tocopherol standards are laboratory reference materials used to identify and quantify the presence of various forms of tocopherols, such as alpha-tocopherol, beta-tocopherol, gamma-tocopherol, and delta-tocopherol, in samples. These standards provide a known concentration of each tocopherol variant, allowing for accurate calibration and measurement of tocopherol levels in test samples.
Automatically generated - may contain errors
Lab products found in correlation
N hexane, by Merck Group (3 mentions)
β sitosterol, by Merck Group (3 mentions)
Methanol, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Campesterol, by Merck Group (2 mentions)
2 propanol, by Merck Group (2 mentions)
2 2 azino bis, by Merck Group (1 mentions)
Methyl tricosanoate, by Larodan (1 mentions)
Diammonium salt, by Merck Group (1 mentions)
2 4 6 tri 2 pyridyl s triazine, by Merck Group (1 mentions)
2 2 diphenyl 1 picryhydrazyl dpph, by Merck Group (1 mentions)
Feso4, by ITW Reagents (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchromane 2 carboxylic acid, by Merck Group (1 mentions)
Anhydrous ethanol, by Merck Group (1 mentions)
α tocopherol, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Sinopharm (1 mentions)
γ tocopherol, by Merck Group (1 mentions)
Brassicasterol, by Merck Group (1 mentions)
β tocopherol, by Merck Group (1 mentions)
δ tocopherol, by Merck Group (1 mentions)
10 protocols using tocopherol standard
1
Antioxidant Capacity Assays Protocol
2
Tocopherol and Phytosterol Quantification
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Tocopherol standards (α-tocopherol, β-tocopherol, γ-tocopherol and δ-tocopherol, purity>98%) were obtained from Sigma-Aldrich Chemical (Nanjing, China). Brassicasterol, campesterol, and β-sitosterol standards (purity≥98%) were also purchased from Sigma-Aldrich Chemical (Nanjing, China). N-hexane, methanol, anhydrous ethanol, acetonitrile (HPLC grade) were obtained from Merck (Nanjing, China). Trolox, AAPH, fluorescein, PBS and other solvents and reagents (analytical grade) were purchased from Sinopharm Chemical Reagent (Shanghai, China).
3
Quantification of Tocopherol Content in Oils
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The alpha-, gamma- and delta- tocopherol content of oil was determined by high-performance liquid chromatography (HPLC). To prepare samples for analyses, 2.5 g of oil were dissolved in 25 mL of HPLC-grade hexane. The tocopherol standards (Sigma-Aldrich, St. Louis, MO, USA) were also prepared at different concentrations (1, 2, 5, 10, 20, 50 and 100 ppm) in hexane. The samples and standards were filtered through 0.45 µm syringe filters into HPLC injection vials. The tocopherols were separated and quantified by injecting 50 µL of diluted standards and samples in a chromatograph equipped with an autosampler AS3000 (Oven temperature = 35 °C, tray temperature = 15 °C), a P1000 spectra system pump (Thermo separation products, San Jose, CA, USA), a Luna 5 µm normal phase column of 250 mm × 4.6 mm internal diameter (Phenomenex, Torrance, CA, USA) and an FP-2020 fluorescence detector (Jasco, Tokyo, Japan). The mobile phase used was 2% (v/v) tetrahydrofuran in n-heptane at a flow rate of 1.8 mL/min. The data were processed using the Chromquest 5.0 data processing software and the results are expressed as µg/g of oil.
4
Fatty Acid Profiling of Green Plum Seeds
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The green plum seed was obtained from Guangdong Green plum Industrial Food Co., ltd. (Guangzhou, China), which is the largest cultivated area of green plums in China. It is the Puning green plum (big nuclear plum) and was collected in May 2021. The green plum seed was dried at ambient temperature (25 °C) for 48 h, and the moisture content was 12 %. The 37 fatty-acid methyl esters standards, tocopherol standards (α-, β-, γ- and δ-tocopherols), phytosterol, and squalene standards (campesterol, stigmasterol, β-sitosterol, Δ5-avenostenol, squalene) were purchased from Sigma-Aldrich Chemical Co. ltd. (Shanghai, China), other reagents and solvents were obtained from Sinopharm Chemical Regent (Shanghai, China).
5
Characterization of Raspberry Seed Oil
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Cold-pressed oil of red raspberry seed (RO, Rubus idaeus) was purchased from Makers Ingredients (York, North Yorkshire, UK). All fatty acids (FA) standards, tocopherol standards, Folin-Ciocalteau reagent, gallic acid, quercetin were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Acetonitrile, hexane, 2-propanol and dimethyl propane of HPLC grade were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Water purification was carried out via the Milli-Q system (Millipore, Bedford, MA, USA). N,N-dimethylformamide (DMF) was purchased from Loba Chemie (Colaba, Mumbai, India). Potassium carbonate (K2CO3) and pyridine were obtained from Sigma Chemical Co. (St. Louis, MO, USA) while chloroform was obtained from Andheri West, Mumbai, Maharashtra, India. The 2-(5-benzoacridine)ethyl-p-toluene sulfonate (BAETS) labeling reagent was synthesized in the laboratory. Unless mentioned, all reagents used were of analytical grade.
6
Antioxidant Capacity Evaluation Protocol
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Tocopherol standards and malondialdehyde were purchased from Merck Ltd. (Darmstadt, Germany). Ammonium iron (II) sulfate, thiobarbituric acid, trichloroacetic acid, hydrochloric acid (37%), and glacial acetic acid were purchased from Panreac (Barcelona, Spain). Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) was purchased from Glentham Life Sciences (Corsham, UK). Dichloromethane and isooctane were obtained from Carlo Erba (Vaul de Reuil, France). Ammonium thiocyanate and chloroform were purchased from Penta (Prague, Czech Republic). Cyclohexane and p-anisidine were obtained from Sigma-Aldrich (St. Louis, Burlington, MA, USA). Ethanol (99.8%) was bought from Fischer Scientific (Loughborough, UK). Hydrogen peroxide (35%) was obtained from Chemco (Malsch, Germany).
7
Quantification of Tocochromanols and Sterols
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The mixture of thirty-seven fatty acid methyl esters (FAME) and N,O-bis-(trimethylsilyl)-trifluoroacetamide (BSTFA) with 1% trimethylchlorosilane (TMCS) were purchased from Supelco (Steinheim, Germany).
Four homologues (α, β, γ and δ) each of tocopherol and tocotrienol HPLC-grade standards (>95% purity) were used for tocochromanol identification and quantification. Tocopherol standards were purchased from Merck (Darmstadt, Germany) and tocotrienol from LGC Standards (Teddington, Middlesex, UK).
High performance liquid chromatography (HPLC) grade solvents, including methanol, 2-propanol, tert-butyl methyl ether, n-hexane, and gas chromatography (GC)-grade standards (≥97% 5α-cholestane, ≥95% brassicasterol, campesterol, stigmasterol, β-sitosterol, cholesterol, squalene) for sterol and squalene analysis were purchased, as well as any other unlisted solvents, reagents and standards, from Sigma-Aldrich (Steinheim, Germany).
Four homologues (α, β, γ and δ) each of tocopherol and tocotrienol HPLC-grade standards (>95% purity) were used for tocochromanol identification and quantification. Tocopherol standards were purchased from Merck (Darmstadt, Germany) and tocotrienol from LGC Standards (Teddington, Middlesex, UK).
High performance liquid chromatography (HPLC) grade solvents, including methanol, 2-propanol, tert-butyl methyl ether, n-hexane, and gas chromatography (GC)-grade standards (≥97% 5α-cholestane, ≥95% brassicasterol, campesterol, stigmasterol, β-sitosterol, cholesterol, squalene) for sterol and squalene analysis were purchased, as well as any other unlisted solvents, reagents and standards, from Sigma-Aldrich (Steinheim, Germany).
8
Tocopherol and Fatty Acid Analysis
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Tocopherol standards, n-hexane and isopropanol were supplied by Merck (Darmstadt, Germany). Standards of fatty acids, anhydrous methanol and 3N methanolic HCl solution were purchased from Sigma- Aldrich (St. Louis, MO, USA). All solvents were of HPLC grade.
9
Quantifying Tocopherols in Liver Lipids
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To examine tocopherol compounds in liver fat, the methodology by Ben Moumen et al. [31 (link)]. We employed high-performance liquid chromatography with diode array detection (HPLC-DAD) for this purpose. Initially, the samples were dissolved in hexane and then separated using an Uptisphere 120 A NH2 silica column (250 mm × 4.6 mm, 5 μm). The mobile phase used was a hexane/isopropanol mixture (99 : 1, v/v) maintained isocratically. The flow rate was set at 1 mL/min, and a UV detector was used to identify the separated compounds at wavelengths of 292, 296, and 298 nm. For quantification, we relied on external calibration, utilizing a commercially available tocopherol standard from Sigma-Aldrich (St. Louis, MO, USA).
10
Camellia Oils: Comprehensive Characterization
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Seventeen camellia oils were collected from different factories in 2017. Among which, nine C. oleifera seed oils and three C. sinensis seed oils were purchased from the local market in Ganzhou city, Jiangxi Province of China; three C. japonica seed oils, two C. sinensis seed oils, extra virgin olive oil, and rapeseed oil were purchased from Tokyo in Japan. Detailed information describing the oil samples is shown in Table 1, which includes the brands, place of production, processing mode, and date of expiry. All the oil samples were stored at -20℃ in a freezer to prevent oxidation before analysis.
Boron trifluoride, tocopherol standard, lanosterol, squalene and 5α-cholestan-3β-ol were purchased from Sigma-Aldrich (St. Louis, MO, USA) . The other reagents were purchased from Wako Pure Chemical Co. (Osaka, Japan) . All chemicals were of analytical reagent grade, and acetone, acetonitrile, n-hexane and 2-propanol were HPLC grade.
Boron trifluoride, tocopherol standard, lanosterol, squalene and 5α-cholestan-3β-ol were purchased from Sigma-Aldrich (St. Louis, MO, USA) . The other reagents were purchased from Wako Pure Chemical Co. (Osaka, Japan) . All chemicals were of analytical reagent grade, and acetone, acetonitrile, n-hexane and 2-propanol were HPLC grade.
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